TY - JOUR

T1 - The role of NADPH oxidase 2 in regulating IL-15 and PGC-1α gene expressions following a period of high intensity interval training

T2 - [The original title and the article is written in Persian!]

AU - Baghersad Renani, L

AU - Ravasi, A A

AU - Shabkhiz, F

AU - Jensen, Thomas Elbenhardt

N1 - CURIS 2019 NEXS 112

PY - 2019

Y1 - 2019

N2 - Introduction: Following trainging, Reactive Oxygen Spices (ROS) play a crucial role in the regulation of signaling pathways and adaptions in skeletal muscle, including mitochondrial biogenesis and improvement in insulin sensitivity a ROS-induced physical activity can also stimulate myokine production in skeletal muscle. The aim of this work was hence to investigate the role of NADPH Oxidase-2 (NOX2), the main source of ROS generation, in contracting muscle in IL-15 gene expression, as well as anychanges in PGC-1α, as a key regulator of mitochondrial biogenesis, following a period of High-intensity Interval Training (HIIT). Materials and Methods: Mice were divided into four groups: Wild type (WT) control (WTc) and training (WTt), NOX-2 deficient (ND) control (NDc) and training (NDt). Training groups performed 6-week HIIT, including 2-min running intervals with 100% of their maximum running speed and 2-min rest with 30% of this speed for a total of 60 min; speed was increased 1m/min each week. IL-15, PGC-1α and NOX2 mRNA levels in gastrocnemius muscle were analyzed using Real time. Results: Basal levels of IL-15 mRNA in NDc group were significantly lower than the other groups. Relative gene expression in WTc, WTt, NDc and NDt changed for IL-15 1, 1.15, 0.49 and 0.99 and for PGC-1α 1, 1.73. 0.76 and 1.4 times, respectively in WTt. Post-training muscle PGC-1α mRNA levels increased significantly. Conclusion: Results indicate that IL-15 gene expression in mouse skeletal muscle is dependent on NOX2. And apparently NOX2 deficiency may result in impaired PGC-1α expression and subsequently diminished mitochondrial biogenesis.

AB - Introduction: Following trainging, Reactive Oxygen Spices (ROS) play a crucial role in the regulation of signaling pathways and adaptions in skeletal muscle, including mitochondrial biogenesis and improvement in insulin sensitivity a ROS-induced physical activity can also stimulate myokine production in skeletal muscle. The aim of this work was hence to investigate the role of NADPH Oxidase-2 (NOX2), the main source of ROS generation, in contracting muscle in IL-15 gene expression, as well as anychanges in PGC-1α, as a key regulator of mitochondrial biogenesis, following a period of High-intensity Interval Training (HIIT). Materials and Methods: Mice were divided into four groups: Wild type (WT) control (WTc) and training (WTt), NOX-2 deficient (ND) control (NDc) and training (NDt). Training groups performed 6-week HIIT, including 2-min running intervals with 100% of their maximum running speed and 2-min rest with 30% of this speed for a total of 60 min; speed was increased 1m/min each week. IL-15, PGC-1α and NOX2 mRNA levels in gastrocnemius muscle were analyzed using Real time. Results: Basal levels of IL-15 mRNA in NDc group were significantly lower than the other groups. Relative gene expression in WTc, WTt, NDc and NDt changed for IL-15 1, 1.15, 0.49 and 0.99 and for PGC-1α 1, 1.73. 0.76 and 1.4 times, respectively in WTt. Post-training muscle PGC-1α mRNA levels increased significantly. Conclusion: Results indicate that IL-15 gene expression in mouse skeletal muscle is dependent on NOX2. And apparently NOX2 deficiency may result in impaired PGC-1α expression and subsequently diminished mitochondrial biogenesis.

KW - High intensity interval training (HIIT)

KW - IL-15

KW - NADPH Oxidase 2

KW - Reactive oxygen spices

KW - Skeletal muscle

UR - http://www.scopus.com/inward/record.url?scp=85063075417&partnerID=8YFLogxK

M3 - Tidsskriftartikel

AN - SCOPUS:85063075417

VL - 20

SP - 263

EP - 272

JO - International Journal of Endocrinology and Metabolism

JF - International Journal of Endocrinology and Metabolism

SN - 1726-913X

IS - 5

ER -