The disposal of sulfide mine tailings in an environmentally sound, yet cost-effective, manner is an issue facing most metal mines. Subaqueous tailing disposal is considered an attractive option for disposal that limits oxygen (O₂) availability within sulfide mine tailings and controlling sulfide oxidation and the resultant acid mine drainage (AMD). Assuming that O₂ profiles represent steady-state conditions, we aim to evaluate the depth-dependent and temperature-dependent rates of O₂ consumption in saturated mine tailings. Measurements include microscale O₂ gradients and diffusivity profiles within columns representing undisturbed mine tailing profiles from an impoundment near Nanisivik Mine in northern Canada. Measurements were made across the diffusive bondary layer (DBL) above the tailing-water interface as well as in the tailings below. Laboratory measurements of O₂ profiles are compared to in situ profiles. From the laboratory results it is possible to evaluate the O₂ flux across the DBL and the depth-integrated O₂ uptake. The results are compared with the average sulfate production rate over 3 months. O₂ uptake in saturated tailings is discussed in relation to O₂ uptake measured in columns after free drainage. The methods applied provide consistent O₂ consumption rates as well as reliable predictions for controlling AMD by keeping taillings under water.