Low- and high-protein diets do not alter ex vivo insulin action in skeletal muscle
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Low- and high-protein diets do not alter ex vivo insulin action in skeletal muscle. / Li, Zhencheng; Rasmussen, Mette Line; Li, Jingwen; Olguín, Carlos Henriquez; Knudsen, Jonas Roland; Søgaard, Ole; Madsen, Agnete Louise Bjerregaard; Jensen, Thomas Elbenhardt.
I: Physiological Reports, Bind 6, Nr. 13, e13798, 2018.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Low- and high-protein diets do not alter ex vivo insulin action in skeletal muscle
AU - Li, Zhencheng
AU - Rasmussen, Mette Line
AU - Li, Jingwen
AU - Olguín, Carlos Henriquez
AU - Knudsen, Jonas Roland
AU - Søgaard, Ole
AU - Madsen, Agnete Louise Bjerregaard
AU - Jensen, Thomas Elbenhardt
N1 - CURIS 2018 NEXS 242
PY - 2018
Y1 - 2018
N2 - A low‐protein high carbohydrate (LPHC) diet and a high‐protein low carbohydrate (HPLC) diet have been reported to positively and negatively regulate whole‐body glucose tolerance and insulin sensitivity, respectively. Skeletal muscle is quantitatively the most important tissue clearing glucose in the postprandial state, but it is unclear if LPHC and HPLC diets directly influence insulin action in skeletal muscle. To test this, mice were placed on control chow diet, LPHC and HPLC diets for 13.5 weeks at which time the submaximal insulin‐stimulated glucose transport and insulin signaling were evaluated in ex vivo incubated oxidative soleus and glycolytic EDL muscle. At the whole‐body level, the diets had the anticipated effects, with LPHC diet improving glucose tolerance and insulin‐sensitivity whereas HPLC diet had the opposite effect. However, neither insulin‐stimulated Akt/TBC1D4 signaling and glucose transport ex vivo, nor cell signaling in vivo were altered by the diets. These data imply that skeletal muscle insulin sensitivity does not contribute to the whole‐body effects of LPHC and HPLC diets on glucose metabolism.
AB - A low‐protein high carbohydrate (LPHC) diet and a high‐protein low carbohydrate (HPLC) diet have been reported to positively and negatively regulate whole‐body glucose tolerance and insulin sensitivity, respectively. Skeletal muscle is quantitatively the most important tissue clearing glucose in the postprandial state, but it is unclear if LPHC and HPLC diets directly influence insulin action in skeletal muscle. To test this, mice were placed on control chow diet, LPHC and HPLC diets for 13.5 weeks at which time the submaximal insulin‐stimulated glucose transport and insulin signaling were evaluated in ex vivo incubated oxidative soleus and glycolytic EDL muscle. At the whole‐body level, the diets had the anticipated effects, with LPHC diet improving glucose tolerance and insulin‐sensitivity whereas HPLC diet had the opposite effect. However, neither insulin‐stimulated Akt/TBC1D4 signaling and glucose transport ex vivo, nor cell signaling in vivo were altered by the diets. These data imply that skeletal muscle insulin sensitivity does not contribute to the whole‐body effects of LPHC and HPLC diets on glucose metabolism.
KW - Dietary protein
KW - Insulin signaling
KW - Skeletal muscle
U2 - 10.14814/phy2.13798
DO - 10.14814/phy2.13798
M3 - Journal article
C2 - 29998629
VL - 6
JO - Physiological Reports
JF - Physiological Reports
SN - 2051-817X
IS - 13
M1 - e13798
ER -
ID: 200293190