Low- and high-protein diets do not alter ex vivo insulin action in skeletal muscle

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Standard

Low- and high-protein diets do not alter ex vivo insulin action in skeletal muscle. / Li, Zhencheng; Rasmussen, Mette Line; Li, Jingwen; Olguín, Carlos Henriquez; Knudsen, Jonas Roland; Søgaard, Ole; Madsen, Agnete Louise Bjerregaard; Jensen, Thomas Elbenhardt.

I: Physiological Reports, Bind 6, Nr. 13, e13798, 2018.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Li, Z, Rasmussen, ML, Li, J, Olguín, CH, Knudsen, JR, Søgaard, O, Madsen, ALB & Jensen, TE 2018, 'Low- and high-protein diets do not alter ex vivo insulin action in skeletal muscle', Physiological Reports, bind 6, nr. 13, e13798. https://doi.org/10.14814/phy2.13798

APA

Li, Z., Rasmussen, M. L., Li, J., Olguín, C. H., Knudsen, J. R., Søgaard, O., Madsen, A. L. B., & Jensen, T. E. (2018). Low- and high-protein diets do not alter ex vivo insulin action in skeletal muscle. Physiological Reports, 6(13), [e13798]. https://doi.org/10.14814/phy2.13798

Vancouver

Li Z, Rasmussen ML, Li J, Olguín CH, Knudsen JR, Søgaard O o.a. Low- and high-protein diets do not alter ex vivo insulin action in skeletal muscle. Physiological Reports. 2018;6(13). e13798. https://doi.org/10.14814/phy2.13798

Author

Li, Zhencheng ; Rasmussen, Mette Line ; Li, Jingwen ; Olguín, Carlos Henriquez ; Knudsen, Jonas Roland ; Søgaard, Ole ; Madsen, Agnete Louise Bjerregaard ; Jensen, Thomas Elbenhardt. / Low- and high-protein diets do not alter ex vivo insulin action in skeletal muscle. I: Physiological Reports. 2018 ; Bind 6, Nr. 13.

Bibtex

@article{38288894da9945cca1c8503bfc14ec6c,
title = "Low- and high-protein diets do not alter ex vivo insulin action in skeletal muscle",
abstract = "A low‐protein high carbohydrate (LPHC) diet and a high‐protein low carbohydrate (HPLC) diet have been reported to positively and negatively regulate whole‐body glucose tolerance and insulin sensitivity, respectively. Skeletal muscle is quantitatively the most important tissue clearing glucose in the postprandial state, but it is unclear if LPHC and HPLC diets directly influence insulin action in skeletal muscle. To test this, mice were placed on control chow diet, LPHC and HPLC diets for 13.5 weeks at which time the submaximal insulin‐stimulated glucose transport and insulin signaling were evaluated in ex vivo incubated oxidative soleus and glycolytic EDL muscle. At the whole‐body level, the diets had the anticipated effects, with LPHC diet improving glucose tolerance and insulin‐sensitivity whereas HPLC diet had the opposite effect. However, neither insulin‐stimulated Akt/TBC1D4 signaling and glucose transport ex vivo, nor cell signaling in vivo were altered by the diets. These data imply that skeletal muscle insulin sensitivity does not contribute to the whole‐body effects of LPHC and HPLC diets on glucose metabolism.",
keywords = "Dietary protein, Insulin signaling, Skeletal muscle",
author = "Zhencheng Li and Rasmussen, {Mette Line} and Jingwen Li and Olgu{\'i}n, {Carlos Henriquez} and Knudsen, {Jonas Roland} and Ole S{\o}gaard and Madsen, {Agnete Louise Bjerregaard} and Jensen, {Thomas Elbenhardt}",
note = "CURIS 2018 NEXS 242",
year = "2018",
doi = "10.14814/phy2.13798",
language = "English",
volume = "6",
journal = "Physiological Reports",
issn = "2051-817X",
publisher = "Wiley Periodicals, Inc.",
number = "13",

}

RIS

TY - JOUR

T1 - Low- and high-protein diets do not alter ex vivo insulin action in skeletal muscle

AU - Li, Zhencheng

AU - Rasmussen, Mette Line

AU - Li, Jingwen

AU - Olguín, Carlos Henriquez

AU - Knudsen, Jonas Roland

AU - Søgaard, Ole

AU - Madsen, Agnete Louise Bjerregaard

AU - Jensen, Thomas Elbenhardt

N1 - CURIS 2018 NEXS 242

PY - 2018

Y1 - 2018

N2 - A low‐protein high carbohydrate (LPHC) diet and a high‐protein low carbohydrate (HPLC) diet have been reported to positively and negatively regulate whole‐body glucose tolerance and insulin sensitivity, respectively. Skeletal muscle is quantitatively the most important tissue clearing glucose in the postprandial state, but it is unclear if LPHC and HPLC diets directly influence insulin action in skeletal muscle. To test this, mice were placed on control chow diet, LPHC and HPLC diets for 13.5 weeks at which time the submaximal insulin‐stimulated glucose transport and insulin signaling were evaluated in ex vivo incubated oxidative soleus and glycolytic EDL muscle. At the whole‐body level, the diets had the anticipated effects, with LPHC diet improving glucose tolerance and insulin‐sensitivity whereas HPLC diet had the opposite effect. However, neither insulin‐stimulated Akt/TBC1D4 signaling and glucose transport ex vivo, nor cell signaling in vivo were altered by the diets. These data imply that skeletal muscle insulin sensitivity does not contribute to the whole‐body effects of LPHC and HPLC diets on glucose metabolism.

AB - A low‐protein high carbohydrate (LPHC) diet and a high‐protein low carbohydrate (HPLC) diet have been reported to positively and negatively regulate whole‐body glucose tolerance and insulin sensitivity, respectively. Skeletal muscle is quantitatively the most important tissue clearing glucose in the postprandial state, but it is unclear if LPHC and HPLC diets directly influence insulin action in skeletal muscle. To test this, mice were placed on control chow diet, LPHC and HPLC diets for 13.5 weeks at which time the submaximal insulin‐stimulated glucose transport and insulin signaling were evaluated in ex vivo incubated oxidative soleus and glycolytic EDL muscle. At the whole‐body level, the diets had the anticipated effects, with LPHC diet improving glucose tolerance and insulin‐sensitivity whereas HPLC diet had the opposite effect. However, neither insulin‐stimulated Akt/TBC1D4 signaling and glucose transport ex vivo, nor cell signaling in vivo were altered by the diets. These data imply that skeletal muscle insulin sensitivity does not contribute to the whole‐body effects of LPHC and HPLC diets on glucose metabolism.

KW - Dietary protein

KW - Insulin signaling

KW - Skeletal muscle

U2 - 10.14814/phy2.13798

DO - 10.14814/phy2.13798

M3 - Journal article

C2 - 29998629

VL - 6

JO - Physiological Reports

JF - Physiological Reports

SN - 2051-817X

IS - 13

M1 - e13798

ER -

ID: 200293190