Kinase activity determination of specific AMPK complexes/heterotrimers in the skeletal muscle

Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt

Standard

Kinase activity determination of specific AMPK complexes/heterotrimers in the skeletal muscle. / Birk, Jesper Bratz; Wojtaszewski, Jørgen.

AMPK: Methods and Protecols. red. / Dietbert Neumann; Benoit Viollet. New York : Humana Press, 2018. s. 215-228 (Methods in Molecular Biology; Nr. 1732).

Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt

Harvard

Birk, JB & Wojtaszewski, J 2018, Kinase activity determination of specific AMPK complexes/heterotrimers in the skeletal muscle. i D Neumann & B Viollet (red), AMPK: Methods and Protecols. Humana Press, New York, Methods in Molecular Biology, nr. 1732, s. 215-228. https://doi.org/10.1007/978-1-4939-7598-3_14

APA

Birk, J. B., & Wojtaszewski, J. (2018). Kinase activity determination of specific AMPK complexes/heterotrimers in the skeletal muscle. I D. Neumann, & B. Viollet (red.), AMPK: Methods and Protecols (s. 215-228). Humana Press. Methods in Molecular Biology Nr. 1732 https://doi.org/10.1007/978-1-4939-7598-3_14

Vancouver

Birk JB, Wojtaszewski J. Kinase activity determination of specific AMPK complexes/heterotrimers in the skeletal muscle. I Neumann D, Viollet B, red., AMPK: Methods and Protecols. New York: Humana Press. 2018. s. 215-228. (Methods in Molecular Biology; Nr. 1732). https://doi.org/10.1007/978-1-4939-7598-3_14

Author

Birk, Jesper Bratz ; Wojtaszewski, Jørgen. / Kinase activity determination of specific AMPK complexes/heterotrimers in the skeletal muscle. AMPK: Methods and Protecols. red. / Dietbert Neumann ; Benoit Viollet. New York : Humana Press, 2018. s. 215-228 (Methods in Molecular Biology; Nr. 1732).

Bibtex

@inbook{ec2d8abb2afb4644ae4cc64fef132aac,
title = "Kinase activity determination of specific AMPK complexes/heterotrimers in the skeletal muscle",
abstract = "Measuring the kinase activity of the 5'-AMP-activated protein kinase (AMPK) is an essential part of understanding the regulation of this metabolic master switch. The AMPK heterotrimer can exist in 12 different constellations with potentially diverse activation patterns. It is therefore important to be able to measure heterotrimer-specific activity to discriminate between these patterns. In this chapter we describe how to measure the AMPK activity of specific heterotrimeric complexes by consecutive immunoprecipitations and how the assay can be performed in a medium throughput fashion using 96-well plates.",
keywords = "Faculty of Science, Immunoprecipitation, AMPK activity, Skeletal muscle, Isoform-specific, Radioactive tracer 33P, AMARA peptide",
author = "Birk, {Jesper Bratz} and J{\o}rgen Wojtaszewski",
note = "CURIS 2018 NEXS 080",
year = "2018",
doi = "10.1007/978-1-4939-7598-3_14",
language = "English",
isbn = "978-1-4939-7597-6",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
number = "1732",
pages = "215--228",
editor = "Dietbert Neumann and Benoit Viollet",
booktitle = "AMPK",
address = "United States",

}

RIS

TY - CHAP

T1 - Kinase activity determination of specific AMPK complexes/heterotrimers in the skeletal muscle

AU - Birk, Jesper Bratz

AU - Wojtaszewski, Jørgen

N1 - CURIS 2018 NEXS 080

PY - 2018

Y1 - 2018

N2 - Measuring the kinase activity of the 5'-AMP-activated protein kinase (AMPK) is an essential part of understanding the regulation of this metabolic master switch. The AMPK heterotrimer can exist in 12 different constellations with potentially diverse activation patterns. It is therefore important to be able to measure heterotrimer-specific activity to discriminate between these patterns. In this chapter we describe how to measure the AMPK activity of specific heterotrimeric complexes by consecutive immunoprecipitations and how the assay can be performed in a medium throughput fashion using 96-well plates.

AB - Measuring the kinase activity of the 5'-AMP-activated protein kinase (AMPK) is an essential part of understanding the regulation of this metabolic master switch. The AMPK heterotrimer can exist in 12 different constellations with potentially diverse activation patterns. It is therefore important to be able to measure heterotrimer-specific activity to discriminate between these patterns. In this chapter we describe how to measure the AMPK activity of specific heterotrimeric complexes by consecutive immunoprecipitations and how the assay can be performed in a medium throughput fashion using 96-well plates.

KW - Faculty of Science

KW - Immunoprecipitation

KW - AMPK activity

KW - Skeletal muscle

KW - Isoform-specific

KW - Radioactive tracer 33P

KW - AMARA peptide

U2 - 10.1007/978-1-4939-7598-3_14

DO - 10.1007/978-1-4939-7598-3_14

M3 - Book chapter

C2 - 29480478

SN - 978-1-4939-7597-6

T3 - Methods in Molecular Biology

SP - 215

EP - 228

BT - AMPK

A2 - Neumann, Dietbert

A2 - Viollet, Benoit

PB - Humana Press

CY - New York

ER -

ID: 191294168