TY - JOUR

T1 - In vitro study of cricket chitosan’s potential as a prebiotic and a promoter of probiotic microorganisms to control pathogenic bacteria in the human gut

AU - Kipkoech, Carolyne

AU - Kinyuru, John N.

AU - Imathiu, Samuel

AU - Meyer-Rochow, Victor Benno

AU - Roos, Nanna

N1 - Funding Information: Financial support for CK came from DANIDA funded by the Ministry of Foreign Affairs Denmark (2013?2018) under the funding no 13-06KU GREEiNSECT project. VBM-R was supported by a grant from the National Research Foundation of Korea (NRF-2018R1A6A1A03024862) to Prof. Chuleui Jung of Andong National University?s Basic Science Research Program. Ethical approval for this study was issued by the Director of Mount Kenya University through Dr Francis W. Muregi of the university?s Ethics Review Committee (ERC) on 09/01/2017 under the number AG422-4492/2015 to Principal Investigator Carolyne Kipkoech. Funding Information: Acknowledgments: The authors wish to acknowledge the support they have received for this research via funds from by DANIDA through the GREEiNSECT project. The authors would also like to express their thanks to Hansen-Denmark through Promaco Limited, Kenya for the provision of probiotic bacterial cultures. Funding Information: Funding: Financial support for CK came from DANIDA funded by the Ministry of Foreign Affairs Denmark (2013–2018) under the funding no 13-06KU GREEiNSECT project. VBM-R was supported by a grant from the National Research Foundation of Korea (NRF-2018R1A6A1A03024862) to Prof. Chuleui Jung of Andong National University’s Basic Science Research Program. Ethical approval for this study was issued by the Director of Mount Kenya University through Dr Francis W. Muregi of the university’s Ethics Review Committee (ERC) on 09/01/2017 under the number AG422-4492/2015 to Principal Investigator Carolyne Kipkoech. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021

Y1 - 2021

N2 - In this study, cricket chitosan was used as a prebiotic. Lactobacillus fermentum, Lactobacillus acidophilus, and Bifidobacterium adolescentis were identified as probiotic bacteria. Cricket chitin was deacetylated to chitosan and added to either De Man Rogosa and Sharpe or Salmonella/Shigella bacterial growth media at the rates of 1%, 5%, 10%, or 20% to obtain chitosan-supplemented media. The growth of the probiotic bacteria was monitored on chitosan-supplemented media after 6, 12, 24, and 48 h upon incubation at 37◦C. Growth of Salmonella typhi in the presence of probiotic bacteria in chitosan-supplemented media was evaluated under similar conditions to those of the growth of probiotic bacteria by measuring growth inhibition zones (in mm) around the bacterial colonies. All chitosan concentrations significantly increased the populations of probiotic bacteria and decreased the populations of pathogenic bacteria. During growth, there was a significant pH change in the media with all probiotic bacteria. Inhibition zones from probiotic bacteria growth supernatant against Salmonella typhi were most apparent at 16 mm and statistically significant in connection with a 10% chitosan concentration. This study suggests cricket-derived chitosan can function as a prebiotic, with an ability to eliminate pathogenic bacteria in the presence of probiotic bacteria.

AB - In this study, cricket chitosan was used as a prebiotic. Lactobacillus fermentum, Lactobacillus acidophilus, and Bifidobacterium adolescentis were identified as probiotic bacteria. Cricket chitin was deacetylated to chitosan and added to either De Man Rogosa and Sharpe or Salmonella/Shigella bacterial growth media at the rates of 1%, 5%, 10%, or 20% to obtain chitosan-supplemented media. The growth of the probiotic bacteria was monitored on chitosan-supplemented media after 6, 12, 24, and 48 h upon incubation at 37◦C. Growth of Salmonella typhi in the presence of probiotic bacteria in chitosan-supplemented media was evaluated under similar conditions to those of the growth of probiotic bacteria by measuring growth inhibition zones (in mm) around the bacterial colonies. All chitosan concentrations significantly increased the populations of probiotic bacteria and decreased the populations of pathogenic bacteria. During growth, there was a significant pH change in the media with all probiotic bacteria. Inhibition zones from probiotic bacteria growth supernatant against Salmonella typhi were most apparent at 16 mm and statistically significant in connection with a 10% chitosan concentration. This study suggests cricket-derived chitosan can function as a prebiotic, with an ability to eliminate pathogenic bacteria in the presence of probiotic bacteria.

KW - Chitin

KW - Chitosan

KW - Diet

KW - Growth inhibition

KW - Human gut bacteria

KW - Pathogenic

KW - Pre-and probiotics

U2 - 10.3390/foods10102310

DO - 10.3390/foods10102310

M3 - Journal article

C2 - 34681361

AN - SCOPUS:85116336106

VL - 10

JO - Foods

JF - Foods

SN - 2304-8158

IS - 10

M1 - 2310

ER -