Hyperinsulinemia does not cause de novo capillary recruitment in rat skeletal muscle

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Standard

Hyperinsulinemia does not cause de novo capillary recruitment in rat skeletal muscle. / Åkerström, Thorbjörn; Goldman, Daniel; Nilsson, Franciska; Milkovich, Stephanie L; Fraser, Graham M; Brand, Christian Lehn; Hellsten, Ylva; Ellis, Christopher G.

I: Microcirculation, Bind 27, Nr. 2, e12593, 2020.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Åkerström, T, Goldman, D, Nilsson, F, Milkovich, SL, Fraser, GM, Brand, CL, Hellsten, Y & Ellis, CG 2020, 'Hyperinsulinemia does not cause de novo capillary recruitment in rat skeletal muscle', Microcirculation, bind 27, nr. 2, e12593. https://doi.org/10.1111/micc.12593

APA

Åkerström, T., Goldman, D., Nilsson, F., Milkovich, S. L., Fraser, G. M., Brand, C. L., ... Ellis, C. G. (2020). Hyperinsulinemia does not cause de novo capillary recruitment in rat skeletal muscle. Microcirculation, 27(2), [e12593]. https://doi.org/10.1111/micc.12593

Vancouver

Åkerström T, Goldman D, Nilsson F, Milkovich SL, Fraser GM, Brand CL o.a. Hyperinsulinemia does not cause de novo capillary recruitment in rat skeletal muscle. Microcirculation. 2020;27(2). e12593. https://doi.org/10.1111/micc.12593

Author

Åkerström, Thorbjörn ; Goldman, Daniel ; Nilsson, Franciska ; Milkovich, Stephanie L ; Fraser, Graham M ; Brand, Christian Lehn ; Hellsten, Ylva ; Ellis, Christopher G. / Hyperinsulinemia does not cause de novo capillary recruitment in rat skeletal muscle. I: Microcirculation. 2020 ; Bind 27, Nr. 2.

Bibtex

@article{3a94776258744cc295c7964849bffadf,
title = "Hyperinsulinemia does not cause de novo capillary recruitment in rat skeletal muscle",
abstract = "Objective: The effect of insulin on blood flow distribution within muscle microvasculature, has been suggested to be important for glucose metabolism. However, the {"}capillary recruitment{"} hypothesis is still controversial and relies on studies using indirect contrast-enhanced ultrasound (CEU) methods.Methods: We studied how hyperinsulinaemia effects capillary blood flow in rat extensor digitorum longus (EDL) muscle during euglycaemic hyperinsulinaemic clamp using intravital video microscopy (IVVM). Additionally, we modelled blood flow and microbubble distribution within the vascular tree under conditions observed during euglycaemic hyperinsulinaemic clamp experiments.Results: Euglycaemic hyperinsulinaemia caused an increase in erythrocyte (80±25{\%}, p<0.01) and plasma (53±12{\%}, p<0.01) flow in rat EDL microvasculature. We found no evidence of de novo capillary recruitment within, or among capillary networks supplied by different terminal arterioles; However, erythrocyte flow became slightly more homogenous. Our computational model predicts that a decrease in asymmetry at arteriolar bifurcations causes redistribution of microbubble flow among capillaries already perfused with erythrocytes and plasma, resulting in 25{\%} more microbubbles flowing through capillaries.Conclusions: Our model suggests increase in CEU signal during hyperinsulinaemia reflects a redistribution of arteriolar flow and not de novo capillary recruitment. IVVM experiments support this prediction showing increases in erythrocyte and plasma flow and not capillary recruitment.",
keywords = "Faculty of Science, Microcirculation, Skeletal muscle, Insulin",
author = "Thorbj{\"o}rn {\AA}kerstr{\"o}m and Daniel Goldman and Franciska Nilsson and Milkovich, {Stephanie L} and Fraser, {Graham M} and Brand, {Christian Lehn} and Ylva Hellsten and Ellis, {Christopher G}",
note = "{\circledC} 2019 John Wiley & Sons Ltd.",
year = "2020",
doi = "10.1111/micc.12593",
language = "English",
volume = "27",
journal = "Microcirculation",
issn = "1073-9688",
publisher = "JohnWiley & Sons Ltd",
number = "2",

}

RIS

TY - JOUR

T1 - Hyperinsulinemia does not cause de novo capillary recruitment in rat skeletal muscle

AU - Åkerström, Thorbjörn

AU - Goldman, Daniel

AU - Nilsson, Franciska

AU - Milkovich, Stephanie L

AU - Fraser, Graham M

AU - Brand, Christian Lehn

AU - Hellsten, Ylva

AU - Ellis, Christopher G

N1 - © 2019 John Wiley & Sons Ltd.

PY - 2020

Y1 - 2020

N2 - Objective: The effect of insulin on blood flow distribution within muscle microvasculature, has been suggested to be important for glucose metabolism. However, the "capillary recruitment" hypothesis is still controversial and relies on studies using indirect contrast-enhanced ultrasound (CEU) methods.Methods: We studied how hyperinsulinaemia effects capillary blood flow in rat extensor digitorum longus (EDL) muscle during euglycaemic hyperinsulinaemic clamp using intravital video microscopy (IVVM). Additionally, we modelled blood flow and microbubble distribution within the vascular tree under conditions observed during euglycaemic hyperinsulinaemic clamp experiments.Results: Euglycaemic hyperinsulinaemia caused an increase in erythrocyte (80±25%, p<0.01) and plasma (53±12%, p<0.01) flow in rat EDL microvasculature. We found no evidence of de novo capillary recruitment within, or among capillary networks supplied by different terminal arterioles; However, erythrocyte flow became slightly more homogenous. Our computational model predicts that a decrease in asymmetry at arteriolar bifurcations causes redistribution of microbubble flow among capillaries already perfused with erythrocytes and plasma, resulting in 25% more microbubbles flowing through capillaries.Conclusions: Our model suggests increase in CEU signal during hyperinsulinaemia reflects a redistribution of arteriolar flow and not de novo capillary recruitment. IVVM experiments support this prediction showing increases in erythrocyte and plasma flow and not capillary recruitment.

AB - Objective: The effect of insulin on blood flow distribution within muscle microvasculature, has been suggested to be important for glucose metabolism. However, the "capillary recruitment" hypothesis is still controversial and relies on studies using indirect contrast-enhanced ultrasound (CEU) methods.Methods: We studied how hyperinsulinaemia effects capillary blood flow in rat extensor digitorum longus (EDL) muscle during euglycaemic hyperinsulinaemic clamp using intravital video microscopy (IVVM). Additionally, we modelled blood flow and microbubble distribution within the vascular tree under conditions observed during euglycaemic hyperinsulinaemic clamp experiments.Results: Euglycaemic hyperinsulinaemia caused an increase in erythrocyte (80±25%, p<0.01) and plasma (53±12%, p<0.01) flow in rat EDL microvasculature. We found no evidence of de novo capillary recruitment within, or among capillary networks supplied by different terminal arterioles; However, erythrocyte flow became slightly more homogenous. Our computational model predicts that a decrease in asymmetry at arteriolar bifurcations causes redistribution of microbubble flow among capillaries already perfused with erythrocytes and plasma, resulting in 25% more microbubbles flowing through capillaries.Conclusions: Our model suggests increase in CEU signal during hyperinsulinaemia reflects a redistribution of arteriolar flow and not de novo capillary recruitment. IVVM experiments support this prediction showing increases in erythrocyte and plasma flow and not capillary recruitment.

KW - Faculty of Science

KW - Microcirculation

KW - Skeletal muscle

KW - Insulin

U2 - 10.1111/micc.12593

DO - 10.1111/micc.12593

M3 - Journal article

C2 - 31605649

VL - 27

JO - Microcirculation

JF - Microcirculation

SN - 1073-9688

IS - 2

M1 - e12593

ER -

ID: 228729197