High-intensity interval, but not endurance training induces muscle fiber type-specific subsarcolemmal lipid droplet size reduction in type 2 diabetic patients
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High-intensity interval, but not endurance training induces muscle fiber type-specific subsarcolemmal lipid droplet size reduction in type 2 diabetic patients. / Koh, Han-Chow E; Ørtenblad, Niels; Winding, Kamilla M; Hellsten, Ylva; Mortensen, Stefan P; Nielsen, Joachim.
I: American Journal of Physiology: Endocrinology and Metabolism, Bind 315, Nr. 5, 2018, s. E872-E884.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - High-intensity interval, but not endurance training induces muscle fiber type-specific subsarcolemmal lipid droplet size reduction in type 2 diabetic patients
AU - Koh, Han-Chow E
AU - Ørtenblad, Niels
AU - Winding, Kamilla M
AU - Hellsten, Ylva
AU - Mortensen, Stefan P
AU - Nielsen, Joachim
N1 - CURIS 2018 NEXS 377
PY - 2018
Y1 - 2018
N2 - This study compared the effects of moderate-intensity endurance training and high-intensity interval training on fiber type-specific subcellular volumetric content and morphology of lipid droplets and mitochondria in skeletal muscles of type 2 diabetic patients. Sixteen sedentary type 2 diabetic patients (57±7 years old) were randomized to complete 11 weeks of either 40-min cycling at 50% peak workload (Endurance, n = 8) or 10 1-min cycling intervals at 95% peak workload separated by 1 min of recovery (High-intensity Interval, n = 8), 3 times per week. Assessments for cardiorespiratory fitness, body composition, glycemic control, together with muscle biopsies were performed before and after the intervention. Morphometric analyses of lipid droplets and mitochondria were conducted in the subcellular fractions of biopsied muscle fibers using quantitative electron microscopy. The training intervention increased cardiorespiratory fitness, lowered fat mass and improved non-fasting glycemic control (P < 0.05), with no difference between training modalities. In the subsarcolemmal space, training decreased lipid droplet volume (P = 0.003), and high-intensity interval, but not endurance training, reduced the size of lipid droplets, specifically in type 2 fibers (P < 0.001). No training-induced change in intermyofibrillar lipid droplets was observed in both fiber types. Subsarcolemmal mitochondrial volume was increased by high-intensity interval (P = 0.02), but not endurance training (P = 0.79). Along with improvement in glycemic control, low volume high-intensity interval training is an alternative time-saving training modality that affects subcellular morphology and volumetric content of lipid droplets in skeletal muscle of type 2 diabetic patients.
AB - This study compared the effects of moderate-intensity endurance training and high-intensity interval training on fiber type-specific subcellular volumetric content and morphology of lipid droplets and mitochondria in skeletal muscles of type 2 diabetic patients. Sixteen sedentary type 2 diabetic patients (57±7 years old) were randomized to complete 11 weeks of either 40-min cycling at 50% peak workload (Endurance, n = 8) or 10 1-min cycling intervals at 95% peak workload separated by 1 min of recovery (High-intensity Interval, n = 8), 3 times per week. Assessments for cardiorespiratory fitness, body composition, glycemic control, together with muscle biopsies were performed before and after the intervention. Morphometric analyses of lipid droplets and mitochondria were conducted in the subcellular fractions of biopsied muscle fibers using quantitative electron microscopy. The training intervention increased cardiorespiratory fitness, lowered fat mass and improved non-fasting glycemic control (P < 0.05), with no difference between training modalities. In the subsarcolemmal space, training decreased lipid droplet volume (P = 0.003), and high-intensity interval, but not endurance training, reduced the size of lipid droplets, specifically in type 2 fibers (P < 0.001). No training-induced change in intermyofibrillar lipid droplets was observed in both fiber types. Subsarcolemmal mitochondrial volume was increased by high-intensity interval (P = 0.02), but not endurance training (P = 0.79). Along with improvement in glycemic control, low volume high-intensity interval training is an alternative time-saving training modality that affects subcellular morphology and volumetric content of lipid droplets in skeletal muscle of type 2 diabetic patients.
KW - Faculty of Science
KW - Lipid droplets
KW - Mitochondria
KW - Skeletal muscle
KW - Stereology
KW - Insulin sensitivity
U2 - 10.1152/ajpendo.00161.2018
DO - 10.1152/ajpendo.00161.2018
M3 - Journal article
C2 - 30016151
VL - 315
SP - E872-E884
JO - American Journal of Physiology - Endocrinology and Metabolism
JF - American Journal of Physiology - Endocrinology and Metabolism
SN - 0193-1849
IS - 5
ER -
ID: 201041787