High Glucosyltransferase B gene Expression and Biofilm Formation of Streptococcus mutans after Exposure to Carbohydrates

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Standard

High Glucosyltransferase B gene Expression and Biofilm Formation of Streptococcus mutans after Exposure to Carbohydrates. / Rezaei, Tohid; Rezaee, Mohammad Ahangarzadeh; Eslami, Hosein; Asgharzadeh, Mohammad; Gholizadeh, Pourya; Yousefi, Leila; Kamounah, Fadhil S.; Khodadadi, Ehsaneh; Kafil, Hossein Samadi.

I: Letters in Applied NanoBioScience, Bind 13, Nr. 1, 33, 30.03.2024.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Rezaei, T, Rezaee, MA, Eslami, H, Asgharzadeh, M, Gholizadeh, P, Yousefi, L, Kamounah, FS, Khodadadi, E & Kafil, HS 2024, 'High Glucosyltransferase B gene Expression and Biofilm Formation of Streptococcus mutans after Exposure to Carbohydrates', Letters in Applied NanoBioScience, bind 13, nr. 1, 33. https://doi.org/10.33263/LIANBS131.033

APA

Rezaei, T., Rezaee, M. A., Eslami, H., Asgharzadeh, M., Gholizadeh, P., Yousefi, L., Kamounah, F. S., Khodadadi, E., & Kafil, H. S. (2024). High Glucosyltransferase B gene Expression and Biofilm Formation of Streptococcus mutans after Exposure to Carbohydrates. Letters in Applied NanoBioScience, 13(1), [33]. https://doi.org/10.33263/LIANBS131.033

Vancouver

Rezaei T, Rezaee MA, Eslami H, Asgharzadeh M, Gholizadeh P, Yousefi L o.a. High Glucosyltransferase B gene Expression and Biofilm Formation of Streptococcus mutans after Exposure to Carbohydrates. Letters in Applied NanoBioScience. 2024 mar. 30;13(1). 33. https://doi.org/10.33263/LIANBS131.033

Author

Rezaei, Tohid ; Rezaee, Mohammad Ahangarzadeh ; Eslami, Hosein ; Asgharzadeh, Mohammad ; Gholizadeh, Pourya ; Yousefi, Leila ; Kamounah, Fadhil S. ; Khodadadi, Ehsaneh ; Kafil, Hossein Samadi. / High Glucosyltransferase B gene Expression and Biofilm Formation of Streptococcus mutans after Exposure to Carbohydrates. I: Letters in Applied NanoBioScience. 2024 ; Bind 13, Nr. 1.

Bibtex

@article{e3c07865f330460e9fc7e132c113f742,
title = "High Glucosyltransferase B gene Expression and Biofilm Formation of Streptococcus mutans after Exposure to Carbohydrates",
abstract = "Streptococcus mutans is the leading cause of dental caries. This bacterium causes the production of insoluble glucans from sucrose to cause the accumulation of this bacterium on the tooth surface and create a biofilm. The GlucosyltransferaseB (GtfB) gene of S. mutans seems to have responsibility for the formation and maturation of biofilm. Starch and sucrose can Increase the count of S. mutans. In this study, we assessed the influence of starch, sucrose, and starch plus sucrose on biofilm formation and gtfB gene Expression of S. mutans ATCC 35668 strain. The biofilm formation was detected by a 96-well microtiter plate crystal violet method containing Luria-Bertani (LB) broth, starch and sucrose. Gene expression of biofilm-forming (GtfB) was quantified through Real-Time PCR. The biofilm formation of S. mutans was increased to 1.54 times in 1% starch concentration, 3.30 times in 1% sucrose, and 2.26 times in the 0.5% starch plus 0.5% sucrose compared to biofilm formation in the media without sugars. The starch, sucrose, and starch plus sucrose carbohydrate also increased its expression by affecting the gtfB gene effective in colonization. These results indicate how carbohydrates directly affect gtfB gene and biofilm formation by S. mutans. Glucosyltransferase B showed great potential for diagnosis and treatment proposes in S. mutans.",
keywords = "biofilm, carbohydrates, decay, GlucosyltransferaseB, Streptococcus mutans",
author = "Tohid Rezaei and Rezaee, {Mohammad Ahangarzadeh} and Hosein Eslami and Mohammad Asgharzadeh and Pourya Gholizadeh and Leila Yousefi and Kamounah, {Fadhil S.} and Ehsaneh Khodadadi and Kafil, {Hossein Samadi}",
note = "Funding Information: This research was funded by Tabriz University of Medical Sciences, grant number 69266. Publisher Copyright: {\textcopyright} 2023 by the authors.",
year = "2024",
month = mar,
day = "30",
doi = "10.33263/LIANBS131.033",
language = "English",
volume = "13",
journal = "Letters in Applied NanoBioScience",
issn = "2284-6808",
publisher = "AMG Transcend Association",
number = "1",

}

RIS

TY - JOUR

T1 - High Glucosyltransferase B gene Expression and Biofilm Formation of Streptococcus mutans after Exposure to Carbohydrates

AU - Rezaei, Tohid

AU - Rezaee, Mohammad Ahangarzadeh

AU - Eslami, Hosein

AU - Asgharzadeh, Mohammad

AU - Gholizadeh, Pourya

AU - Yousefi, Leila

AU - Kamounah, Fadhil S.

AU - Khodadadi, Ehsaneh

AU - Kafil, Hossein Samadi

N1 - Funding Information: This research was funded by Tabriz University of Medical Sciences, grant number 69266. Publisher Copyright: © 2023 by the authors.

PY - 2024/3/30

Y1 - 2024/3/30

N2 - Streptococcus mutans is the leading cause of dental caries. This bacterium causes the production of insoluble glucans from sucrose to cause the accumulation of this bacterium on the tooth surface and create a biofilm. The GlucosyltransferaseB (GtfB) gene of S. mutans seems to have responsibility for the formation and maturation of biofilm. Starch and sucrose can Increase the count of S. mutans. In this study, we assessed the influence of starch, sucrose, and starch plus sucrose on biofilm formation and gtfB gene Expression of S. mutans ATCC 35668 strain. The biofilm formation was detected by a 96-well microtiter plate crystal violet method containing Luria-Bertani (LB) broth, starch and sucrose. Gene expression of biofilm-forming (GtfB) was quantified through Real-Time PCR. The biofilm formation of S. mutans was increased to 1.54 times in 1% starch concentration, 3.30 times in 1% sucrose, and 2.26 times in the 0.5% starch plus 0.5% sucrose compared to biofilm formation in the media without sugars. The starch, sucrose, and starch plus sucrose carbohydrate also increased its expression by affecting the gtfB gene effective in colonization. These results indicate how carbohydrates directly affect gtfB gene and biofilm formation by S. mutans. Glucosyltransferase B showed great potential for diagnosis and treatment proposes in S. mutans.

AB - Streptococcus mutans is the leading cause of dental caries. This bacterium causes the production of insoluble glucans from sucrose to cause the accumulation of this bacterium on the tooth surface and create a biofilm. The GlucosyltransferaseB (GtfB) gene of S. mutans seems to have responsibility for the formation and maturation of biofilm. Starch and sucrose can Increase the count of S. mutans. In this study, we assessed the influence of starch, sucrose, and starch plus sucrose on biofilm formation and gtfB gene Expression of S. mutans ATCC 35668 strain. The biofilm formation was detected by a 96-well microtiter plate crystal violet method containing Luria-Bertani (LB) broth, starch and sucrose. Gene expression of biofilm-forming (GtfB) was quantified through Real-Time PCR. The biofilm formation of S. mutans was increased to 1.54 times in 1% starch concentration, 3.30 times in 1% sucrose, and 2.26 times in the 0.5% starch plus 0.5% sucrose compared to biofilm formation in the media without sugars. The starch, sucrose, and starch plus sucrose carbohydrate also increased its expression by affecting the gtfB gene effective in colonization. These results indicate how carbohydrates directly affect gtfB gene and biofilm formation by S. mutans. Glucosyltransferase B showed great potential for diagnosis and treatment proposes in S. mutans.

KW - biofilm

KW - carbohydrates

KW - decay

KW - GlucosyltransferaseB

KW - Streptococcus mutans

U2 - 10.33263/LIANBS131.033

DO - 10.33263/LIANBS131.033

M3 - Journal article

AN - SCOPUS:85169450268

VL - 13

JO - Letters in Applied NanoBioScience

JF - Letters in Applied NanoBioScience

SN - 2284-6808

IS - 1

M1 - 33

ER -

ID: 381220660