FAT/CD36 is localized in sarcolemma and in vesicle-like structures in subsarcolemma regions but not in mitochondria
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FAT/CD36 is localized in sarcolemma and in vesicle-like structures in subsarcolemma regions but not in mitochondria. / Jeppesen, Jacob; Mogensen, Martin; Prats, Clara; Sahlin, Kent; Madsen, Klavs; Kiens, Bente.
I: Journal of Lipid Research, Bind 51, Nr. 6, 2010, s. 1504-1512.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - FAT/CD36 is localized in sarcolemma and in vesicle-like structures in subsarcolemma regions but not in mitochondria
AU - Jeppesen, Jacob
AU - Mogensen, Martin
AU - Prats, Clara
AU - Sahlin, Kent
AU - Madsen, Klavs
AU - Kiens, Bente
N1 - CURIS 2010 5200 010
PY - 2010
Y1 - 2010
N2 - The primary aim of the present study was to investigate in which cellular compartments FAT/CD36 is localized. Intact and fully functional skeletal muscle mitochondria were isolated from lean and obese female Zucker rats and from 10 healthy male individuals. FAT/CD36 could not be detected in the isolated mitochondria, whereas the mitochondrial marker F1ATPase-beta was clearly detected using immunoblotting. Lack of markers for other membrane structures indicated that the mitochondria were not contaminated with membranes known to contain FAT/CD36 . In addition, fluorescence immunocytochemistry was performed on single muscle fibers dissected from soleus muscle of lean and obese Zucker rats and from the vastus lateralis muscle from humans. Co-staining against FAT/CD36 and MitoNEET clearly show that FAT/CD36 is highly present in sarcolemma and it also associates with some vesicle-like intracellular compartments. However, FAT/CD36 protein was not detected in mitochondrial membranes, supporting the biochemical findings. Based on the presented data FAT/CD36 seems to be abundantly expressed in sarcolemma and in vesicle-like structure throughout the muscle cell. However, FAT/CD36 was not present in mitochondria in rat or human skeletal muscle. Thus, the functional role of FAT/CD36 in lipid transport seems primarily to be allocated to the plasma membrane in skeletal muscle.
AB - The primary aim of the present study was to investigate in which cellular compartments FAT/CD36 is localized. Intact and fully functional skeletal muscle mitochondria were isolated from lean and obese female Zucker rats and from 10 healthy male individuals. FAT/CD36 could not be detected in the isolated mitochondria, whereas the mitochondrial marker F1ATPase-beta was clearly detected using immunoblotting. Lack of markers for other membrane structures indicated that the mitochondria were not contaminated with membranes known to contain FAT/CD36 . In addition, fluorescence immunocytochemistry was performed on single muscle fibers dissected from soleus muscle of lean and obese Zucker rats and from the vastus lateralis muscle from humans. Co-staining against FAT/CD36 and MitoNEET clearly show that FAT/CD36 is highly present in sarcolemma and it also associates with some vesicle-like intracellular compartments. However, FAT/CD36 protein was not detected in mitochondrial membranes, supporting the biochemical findings. Based on the presented data FAT/CD36 seems to be abundantly expressed in sarcolemma and in vesicle-like structure throughout the muscle cell. However, FAT/CD36 was not present in mitochondria in rat or human skeletal muscle. Thus, the functional role of FAT/CD36 in lipid transport seems primarily to be allocated to the plasma membrane in skeletal muscle.
U2 - 10.1194/jlr.M003756
DO - 10.1194/jlr.M003756
M3 - Journal article
C2 - 20023206
VL - 51
SP - 1504
EP - 1512
JO - Journal of Lipid Research
JF - Journal of Lipid Research
SN - 0022-2275
IS - 6
ER -
ID: 17111684