Distinctive profile of monomeric and polymeric anti-SSA/Ro52 immunoglobulin A1 isoforms in saliva of patients with primary Sjögren's syndrome and Sicca

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Standard

Distinctive profile of monomeric and polymeric anti-SSA/Ro52 immunoglobulin A1 isoforms in saliva of patients with primary Sjögren's syndrome and Sicca. / Chiang, Samantha; Grogan, Tristan; Kamounah, Sarah; Wei, Fang; Tayob, Nabihah; Kim, Ju Yeon; Park, Jin Kyun; Akin, David; Elashoff, David A.; Pedersen, Anne Marie Lynge; Song, Yeong Wook; Wong, David T. W.; Chia, David.

I: RMD Open, Bind 10, Nr. 2, e003666, 2024.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Chiang, S, Grogan, T, Kamounah, S, Wei, F, Tayob, N, Kim, JY, Park, JK, Akin, D, Elashoff, DA, Pedersen, AML, Song, YW, Wong, DTW & Chia, D 2024, 'Distinctive profile of monomeric and polymeric anti-SSA/Ro52 immunoglobulin A1 isoforms in saliva of patients with primary Sjögren's syndrome and Sicca', RMD Open, bind 10, nr. 2, e003666. https://doi.org/10.1136/rmdopen-2023-003666

APA

Chiang, S., Grogan, T., Kamounah, S., Wei, F., Tayob, N., Kim, J. Y., Park, J. K., Akin, D., Elashoff, D. A., Pedersen, A. M. L., Song, Y. W., Wong, D. T. W., & Chia, D. (2024). Distinctive profile of monomeric and polymeric anti-SSA/Ro52 immunoglobulin A1 isoforms in saliva of patients with primary Sjögren's syndrome and Sicca. RMD Open, 10(2), [e003666]. https://doi.org/10.1136/rmdopen-2023-003666

Vancouver

Chiang S, Grogan T, Kamounah S, Wei F, Tayob N, Kim JY o.a. Distinctive profile of monomeric and polymeric anti-SSA/Ro52 immunoglobulin A1 isoforms in saliva of patients with primary Sjögren's syndrome and Sicca. RMD Open. 2024;10(2). e003666. https://doi.org/10.1136/rmdopen-2023-003666

Author

Chiang, Samantha ; Grogan, Tristan ; Kamounah, Sarah ; Wei, Fang ; Tayob, Nabihah ; Kim, Ju Yeon ; Park, Jin Kyun ; Akin, David ; Elashoff, David A. ; Pedersen, Anne Marie Lynge ; Song, Yeong Wook ; Wong, David T. W. ; Chia, David. / Distinctive profile of monomeric and polymeric anti-SSA/Ro52 immunoglobulin A1 isoforms in saliva of patients with primary Sjögren's syndrome and Sicca. I: RMD Open. 2024 ; Bind 10, Nr. 2.

Bibtex

@article{489ed3af867c4d57b0ca76463cad3612,
title = "Distinctive profile of monomeric and polymeric anti-SSA/Ro52 immunoglobulin A1 isoforms in saliva of patients with primary Sj{\"o}gren's syndrome and Sicca",
abstract = "OBJECTIVE: Primary Sj{\"o}gren's syndrome (pSS) is the second most common chronic autoimmune connective tissue disease. Autoantibodies, immunoglobulin (IgG) anti-SSA/Ro, in serum is a key diagnostic feature of pSS. Since pSS is a disease of the salivary gland, we investigated anti-SSA/Ro52 in saliva.METHODS: Using a novel electrochemical detection platform, Electric Field-Induced Release and Measurement, we measured IgG/M/A, IgG, IgA, IgA isotypes (IgA1 and IgA2) and IgA1 subclasses (polymeric and monomeric IgA1) to anti-SSA/Ro52 in saliva supernatant of 34 pSS, 35 dry eyes and dry mouth (patients with Sicca) and 41 health controls.RESULTS: Saliva IgG/M/A, IgG, IgA, IgA isotypes and IgA1 subclasses to anti-SSA/Ro52 differed significantly between pSS, non-pSS Sicca and healthy subjects. Elevated monomeric IgA1 was observed in patients with non-pSS Sicca while elevated polymeric IgA1 (pIgA1) was observed in patients with pSS. Salivary polymeric but not monomeric IgA1 (mIgA1) isoform correlated with focus score (r2=0.467, p=0.001) CONCLUSIONS: Salivary anti-Ro52 polymeric IgA1 isoform is associated with glandular inflammation in pSS, while salivary monomeric IgA1 is associated with Sicca. Whether IgA1 isotope switching plays a role in the progression of the Sicca to pSS warrants further investigation.",
keywords = "Humans, Saliva, Sjogren's Syndrome/diagnosis, Immunoglobulin A, Autoantibodies, Immunoglobulin G",
author = "Samantha Chiang and Tristan Grogan and Sarah Kamounah and Fang Wei and Nabihah Tayob and Kim, {Ju Yeon} and Park, {Jin Kyun} and David Akin and Elashoff, {David A.} and Pedersen, {Anne Marie Lynge} and Song, {Yeong Wook} and Wong, {David T. W.} and David Chia",
note = "{\textcopyright} Author(s) (or their employer(s)) 2024. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.",
year = "2024",
doi = "10.1136/rmdopen-2023-003666",
language = "English",
volume = "10",
journal = "RMD Open",
issn = "2056-5933",
publisher = "BMJ Publishing Group",
number = "2",

}

RIS

TY - JOUR

T1 - Distinctive profile of monomeric and polymeric anti-SSA/Ro52 immunoglobulin A1 isoforms in saliva of patients with primary Sjögren's syndrome and Sicca

AU - Chiang, Samantha

AU - Grogan, Tristan

AU - Kamounah, Sarah

AU - Wei, Fang

AU - Tayob, Nabihah

AU - Kim, Ju Yeon

AU - Park, Jin Kyun

AU - Akin, David

AU - Elashoff, David A.

AU - Pedersen, Anne Marie Lynge

AU - Song, Yeong Wook

AU - Wong, David T. W.

AU - Chia, David

N1 - © Author(s) (or their employer(s)) 2024. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

PY - 2024

Y1 - 2024

N2 - OBJECTIVE: Primary Sjögren's syndrome (pSS) is the second most common chronic autoimmune connective tissue disease. Autoantibodies, immunoglobulin (IgG) anti-SSA/Ro, in serum is a key diagnostic feature of pSS. Since pSS is a disease of the salivary gland, we investigated anti-SSA/Ro52 in saliva.METHODS: Using a novel electrochemical detection platform, Electric Field-Induced Release and Measurement, we measured IgG/M/A, IgG, IgA, IgA isotypes (IgA1 and IgA2) and IgA1 subclasses (polymeric and monomeric IgA1) to anti-SSA/Ro52 in saliva supernatant of 34 pSS, 35 dry eyes and dry mouth (patients with Sicca) and 41 health controls.RESULTS: Saliva IgG/M/A, IgG, IgA, IgA isotypes and IgA1 subclasses to anti-SSA/Ro52 differed significantly between pSS, non-pSS Sicca and healthy subjects. Elevated monomeric IgA1 was observed in patients with non-pSS Sicca while elevated polymeric IgA1 (pIgA1) was observed in patients with pSS. Salivary polymeric but not monomeric IgA1 (mIgA1) isoform correlated with focus score (r2=0.467, p=0.001) CONCLUSIONS: Salivary anti-Ro52 polymeric IgA1 isoform is associated with glandular inflammation in pSS, while salivary monomeric IgA1 is associated with Sicca. Whether IgA1 isotope switching plays a role in the progression of the Sicca to pSS warrants further investigation.

AB - OBJECTIVE: Primary Sjögren's syndrome (pSS) is the second most common chronic autoimmune connective tissue disease. Autoantibodies, immunoglobulin (IgG) anti-SSA/Ro, in serum is a key diagnostic feature of pSS. Since pSS is a disease of the salivary gland, we investigated anti-SSA/Ro52 in saliva.METHODS: Using a novel electrochemical detection platform, Electric Field-Induced Release and Measurement, we measured IgG/M/A, IgG, IgA, IgA isotypes (IgA1 and IgA2) and IgA1 subclasses (polymeric and monomeric IgA1) to anti-SSA/Ro52 in saliva supernatant of 34 pSS, 35 dry eyes and dry mouth (patients with Sicca) and 41 health controls.RESULTS: Saliva IgG/M/A, IgG, IgA, IgA isotypes and IgA1 subclasses to anti-SSA/Ro52 differed significantly between pSS, non-pSS Sicca and healthy subjects. Elevated monomeric IgA1 was observed in patients with non-pSS Sicca while elevated polymeric IgA1 (pIgA1) was observed in patients with pSS. Salivary polymeric but not monomeric IgA1 (mIgA1) isoform correlated with focus score (r2=0.467, p=0.001) CONCLUSIONS: Salivary anti-Ro52 polymeric IgA1 isoform is associated with glandular inflammation in pSS, while salivary monomeric IgA1 is associated with Sicca. Whether IgA1 isotope switching plays a role in the progression of the Sicca to pSS warrants further investigation.

KW - Humans

KW - Saliva

KW - Sjogren's Syndrome/diagnosis

KW - Immunoglobulin A

KW - Autoantibodies

KW - Immunoglobulin G

U2 - 10.1136/rmdopen-2023-003666

DO - 10.1136/rmdopen-2023-003666

M3 - Journal article

C2 - 38599651

VL - 10

JO - RMD Open

JF - RMD Open

SN - 2056-5933

IS - 2

M1 - e003666

ER -

ID: 388745308