Direct interaction between the Arabidopsis disease resistance signaling proteins, EDS1 and PAD4
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Direct interaction between the Arabidopsis disease resistance signaling proteins, EDS1 and PAD4. / Feys, Bart J.; Moisan, Lisa J.; Newman, Mari Anne; Parker, Jane E.
I: EMBO Journal, Bind 20, Nr. 19, 01.10.2001, s. 5400-5411.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Direct interaction between the Arabidopsis disease resistance signaling proteins, EDS1 and PAD4
AU - Feys, Bart J.
AU - Moisan, Lisa J.
AU - Newman, Mari Anne
AU - Parker, Jane E.
PY - 2001/10/1
Y1 - 2001/10/1
N2 - The Arabidopsis EDS1 and PAD4 genes encode lipase-like proteins that function in resistance (R) gene-mediated and basal plant disease resistance. Phenotypic analysis of eds1 and pad4 null mutants shows that EDS1 and PAD4 are required for resistance conditioned by the same spectrum of R genes but fulfil distinct roles within the defence pathway. EDS1 is essential for elaboration of the plant hypersensitive response, whereas EDS1 and PAD4 are both required for accumulation of the plant defence-potentiating molecule, salicylic acid. EDS1 is necessary for pathogen-induced PAD4 mRNA accumulation, whereas mutations in PAD4 or depletion of salicylic acid only partially compromise EDS1 expression. Yeast two-hybrid analysis reveals that EDS1 can dimerize and interact with PAD4. However, EDS1 dimerization is mediated by different domains to those involved in EDS1-PAD4 association. Co-immunoprecipitation experiments show that EDS1 and PAD4 proteins interact in healthy and pathogen-challenged plant cells. We propose two functions for EDS1. The first is required early in plant defence, independently of PAD4. The second recruits PAD4 in the amplification of defences, possibly by direct EDS1-PAD4 association.
AB - The Arabidopsis EDS1 and PAD4 genes encode lipase-like proteins that function in resistance (R) gene-mediated and basal plant disease resistance. Phenotypic analysis of eds1 and pad4 null mutants shows that EDS1 and PAD4 are required for resistance conditioned by the same spectrum of R genes but fulfil distinct roles within the defence pathway. EDS1 is essential for elaboration of the plant hypersensitive response, whereas EDS1 and PAD4 are both required for accumulation of the plant defence-potentiating molecule, salicylic acid. EDS1 is necessary for pathogen-induced PAD4 mRNA accumulation, whereas mutations in PAD4 or depletion of salicylic acid only partially compromise EDS1 expression. Yeast two-hybrid analysis reveals that EDS1 can dimerize and interact with PAD4. However, EDS1 dimerization is mediated by different domains to those involved in EDS1-PAD4 association. Co-immunoprecipitation experiments show that EDS1 and PAD4 proteins interact in healthy and pathogen-challenged plant cells. We propose two functions for EDS1. The first is required early in plant defence, independently of PAD4. The second recruits PAD4 in the amplification of defences, possibly by direct EDS1-PAD4 association.
KW - Arabidopsis
KW - Dimerization
KW - EDS1
KW - PAD4
KW - Salicylic acid
UR - http://www.scopus.com/inward/record.url?scp=0035477801&partnerID=8YFLogxK
U2 - 10.1093/emboj/20.19.5400
DO - 10.1093/emboj/20.19.5400
M3 - Journal article
C2 - 11574472
AN - SCOPUS:0035477801
VL - 20
SP - 5400
EP - 5411
JO - E M B O Journal
JF - E M B O Journal
SN - 0261-4189
IS - 19
ER -
ID: 380058897