Design and Purification of Tag/Catcher AP205-Based Capsid Virus-Like Particle Vaccines
Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
Capsid virus-like particles (cVLPs), assembled from viral coat proteins, are used as therapeutic cargo delivery vehicles as well as molecular scaffolds for display of vaccine antigens. A versatile vaccine platform has been developed based on the Acinetobacter phage AP205 cVLP, which has been shown to significantly improve antigen-specific antibody responses. This modular cVLP platform exploits a split-protein (Tag/Catcher) conjugation system to enable high-density, unidirectional antigen display. Accordingly, protein antigens can be independently expressed and quality-checked prior to conjugation to pre-assembled cVLPs. Here, we describe considerations for the design of vaccine antigens with genetically fused split-protein (Tag or Catcher) binding partners and provide protocols for the expression and purification of corresponding Tag- or Catcher-AP205 cVLPs from E.coli. Finally, we describe a generic protocol for the formulation and quality assessment of experimental/pre-clinical AP205 cVLP-based vaccines.
Originalsprog | Engelsk |
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Titel | Therapeutic Proteins : Methods and Protocols |
Forlag | Humana Press |
Publikationsdato | 2024 |
Sider | 127-141 |
ISBN (Trykt) | 978-1-0716-3468-4 |
ISBN (Elektronisk) | 978-1-0716-3469-1 |
DOI | |
Status | Udgivet - 2024 |
Navn | Methods in Molecular Biology |
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Vol/bind | 2720 |
ISSN | 1064-3745 |
Bibliografisk note
Funding Information:
This research was funded by Innovation Fund Denmark, grant number 8088-00032B. We would like to acknowledge all past and present members of the Adaptvac VLP research group for development and optimization of these protocols.
Publisher Copyright:
© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.
ID: 378966950