Comparing the binding properties of peptides mimicking the Envelope protein of SARS-CoV and SARS-CoV-2 to the PDZ domain of the tight junction-associated PALS1 protein

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Comparing the binding properties of peptides mimicking the Envelope protein of SARS-CoV and SARS-CoV-2 to the PDZ domain of the tight junction-associated PALS1 protein. / Toto, Angelo; Ma, Sana; Malagrinò, Francesca; Visconti, Lorenzo; Pagano, Livia; Stromgaard, Kristian; Gianni, Stefano.

I: Protein Science, Bind 29, Nr. 10, 2020, s. 2038-2042.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Toto, A, Ma, S, Malagrinò, F, Visconti, L, Pagano, L, Stromgaard, K & Gianni, S 2020, 'Comparing the binding properties of peptides mimicking the Envelope protein of SARS-CoV and SARS-CoV-2 to the PDZ domain of the tight junction-associated PALS1 protein', Protein Science, bind 29, nr. 10, s. 2038-2042. https://doi.org/10.1002/pro.3936

APA

Toto, A., Ma, S., Malagrinò, F., Visconti, L., Pagano, L., Stromgaard, K., & Gianni, S. (2020). Comparing the binding properties of peptides mimicking the Envelope protein of SARS-CoV and SARS-CoV-2 to the PDZ domain of the tight junction-associated PALS1 protein. Protein Science, 29(10), 2038-2042. https://doi.org/10.1002/pro.3936

Vancouver

Toto A, Ma S, Malagrinò F, Visconti L, Pagano L, Stromgaard K o.a. Comparing the binding properties of peptides mimicking the Envelope protein of SARS-CoV and SARS-CoV-2 to the PDZ domain of the tight junction-associated PALS1 protein. Protein Science. 2020;29(10):2038-2042. https://doi.org/10.1002/pro.3936

Author

Toto, Angelo ; Ma, Sana ; Malagrinò, Francesca ; Visconti, Lorenzo ; Pagano, Livia ; Stromgaard, Kristian ; Gianni, Stefano. / Comparing the binding properties of peptides mimicking the Envelope protein of SARS-CoV and SARS-CoV-2 to the PDZ domain of the tight junction-associated PALS1 protein. I: Protein Science. 2020 ; Bind 29, Nr. 10. s. 2038-2042.

Bibtex

@article{0aa7031cf83f4fa7ad58b011b50f55f2,
title = "Comparing the binding properties of peptides mimicking the Envelope protein of SARS-CoV and SARS-CoV-2 to the PDZ domain of the tight junction-associated PALS1 protein",
abstract = "The Envelope protein (E) is one of the four structural proteins encoded by the genome of SARS-CoV and SARS-CoV-2 Coronaviruses. It is an integral membrane protein, highly expressed in the host cell, which is known to have an important role in Coronaviruses maturation, assembly and virulence. The E protein presents a PDZ-binding motif at its C-terminus. One of the key interactors of the E protein in the intracellular environment is the PDZ containing protein PALS1. This interaction is known to play a key role in the SARS-CoV pathology and suspected to affect the integrity of the lung epithelia. In this paper we measured and compared the affinity of peptides mimicking the E protein from SARS-CoV and SARS-CoV-2 for the PDZ domain of PALS1, through equilibrium and kinetic binding experiments. Our results support the hypothesis that the increased virulence of SARS-CoV-2 compared to SARS-CoV may rely on the increased affinity of its Envelope protein for PALS1.",
author = "Angelo Toto and Sana Ma and Francesca Malagrin{\`o} and Lorenzo Visconti and Livia Pagano and Kristian Stromgaard and Stefano Gianni",
note = "{\textcopyright} 2020 The Protein Society.",
year = "2020",
doi = "10.1002/pro.3936",
language = "English",
volume = "29",
pages = "2038--2042",
journal = "Protein Science",
issn = "0961-8368",
publisher = "Wiley-Blackwell",
number = "10",

}

RIS

TY - JOUR

T1 - Comparing the binding properties of peptides mimicking the Envelope protein of SARS-CoV and SARS-CoV-2 to the PDZ domain of the tight junction-associated PALS1 protein

AU - Toto, Angelo

AU - Ma, Sana

AU - Malagrinò, Francesca

AU - Visconti, Lorenzo

AU - Pagano, Livia

AU - Stromgaard, Kristian

AU - Gianni, Stefano

N1 - © 2020 The Protein Society.

PY - 2020

Y1 - 2020

N2 - The Envelope protein (E) is one of the four structural proteins encoded by the genome of SARS-CoV and SARS-CoV-2 Coronaviruses. It is an integral membrane protein, highly expressed in the host cell, which is known to have an important role in Coronaviruses maturation, assembly and virulence. The E protein presents a PDZ-binding motif at its C-terminus. One of the key interactors of the E protein in the intracellular environment is the PDZ containing protein PALS1. This interaction is known to play a key role in the SARS-CoV pathology and suspected to affect the integrity of the lung epithelia. In this paper we measured and compared the affinity of peptides mimicking the E protein from SARS-CoV and SARS-CoV-2 for the PDZ domain of PALS1, through equilibrium and kinetic binding experiments. Our results support the hypothesis that the increased virulence of SARS-CoV-2 compared to SARS-CoV may rely on the increased affinity of its Envelope protein for PALS1.

AB - The Envelope protein (E) is one of the four structural proteins encoded by the genome of SARS-CoV and SARS-CoV-2 Coronaviruses. It is an integral membrane protein, highly expressed in the host cell, which is known to have an important role in Coronaviruses maturation, assembly and virulence. The E protein presents a PDZ-binding motif at its C-terminus. One of the key interactors of the E protein in the intracellular environment is the PDZ containing protein PALS1. This interaction is known to play a key role in the SARS-CoV pathology and suspected to affect the integrity of the lung epithelia. In this paper we measured and compared the affinity of peptides mimicking the E protein from SARS-CoV and SARS-CoV-2 for the PDZ domain of PALS1, through equilibrium and kinetic binding experiments. Our results support the hypothesis that the increased virulence of SARS-CoV-2 compared to SARS-CoV may rely on the increased affinity of its Envelope protein for PALS1.

U2 - 10.1002/pro.3936

DO - 10.1002/pro.3936

M3 - Journal article

C2 - 32822073

VL - 29

SP - 2038

EP - 2042

JO - Protein Science

JF - Protein Science

SN - 0961-8368

IS - 10

ER -

ID: 249062244