Chemoenzymatic indican for light-driven denim dyeing
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Dokumenter
- Fulltext
Forlagets udgivne version, 2,1 MB, PDF-dokument
Blue denim, a billion-dollar industry, is currently dyed with indigo in an unsustainable process requiring harsh reducing and alkaline chemicals. Forming indigo directly in the yarn through indican (indoxyl-β-glucoside) is a promising alternative route with mild conditions. Indican eliminates the requirement for reducing agent while still ending as indigo, the only known molecule yielding the unique hue of blue denim. However, a bulk source of indican is missing. Here, we employ enzyme and process engineering guided by techno-economic analyses to develop an economically viable drop-in indican synthesis technology. Rational engineering of PtUGT1, a glycosyltransferase from the indigo plant, alleviated the severe substrate inactivation observed with the wildtype enzyme at the titers needed for bulk production. We further describe a mild, light-driven dyeing process. Finally, we conduct techno-economic, social sustainability, and comparative life-cycle assessments. These indicate that the presented technologies have the potential to significantly reduce environmental impacts from blue denim dyeing with only a modest cost increase.
Originalsprog | Engelsk |
---|---|
Artikelnummer | 1489 |
Tidsskrift | Nature Communications |
Vol/bind | 15 |
Udgave nummer | 1 |
Antal sider | 11 |
ISSN | 2041-1723 |
DOI | |
Status | Udgivet - 2024 |
Bibliografisk note
Publisher Copyright:
© The Author(s) 2024.
ID: 385221497