TY - JOUR

T1 - A period of 10 weeks of increased protein consumption does not alter faecal microbiota or volatile metabolites in healthy older men

T2 - A randomised controlled trial

AU - Mitchell, Sarah M

AU - McKenzie, E J

AU - Mitchell, Cameron J

AU - Milan, Amber M

AU - Zeng, Nina

AU - D'Souza, Randall F

AU - Ramzan, Farha

AU - Sharma, Pankaja

AU - Rettedal, Elizabeth

AU - Knowles, Scott O

AU - Roy, Nicole C

AU - Sjödin, Anders Mikael

AU - Wagner, Karl-Heinz

AU - O'Sullivan, J M

AU - Cameron-Smith, David

N1 - CURIS 2020 NEXS 234

PY - 2020

Y1 - 2020

N2 - Diet has a major influence on the composition and metabolic output of the gut microbiome. Higher-protein diets are often recommended for older consumers; however, the effect of high-protein diets on the gut microbiota and faecal volatile organic compounds (VOC) of elderly participants is unknown. The purpose of the study was to establish if the faecal microbiota composition and VOC in older men are different after a diet containing the recommended dietary intake (RDA) of protein compared with a diet containing twice the RDA (2RDA). Healthy males (74·2 (SD·6) years; n 28) were randomised to consume the RDA of protein (0·8 g protein/kg body weight per d) or 2RDA, for 10 weeks. Dietary protein was provided via whole foods rather than supplementation or fortification. The diets were matched for dietary fibre from fruit and vegetables. Faecal samples were collected pre- and post-intervention for microbiota profiling by 16S ribosomal RNA amplicon sequencing and VOC analysis by head space/solid-phase microextraction/GC-MS. After correcting for multiple comparisons, no significant differences in the abundance of faecal microbiota or VOC associated with protein fermentation were evident between the RDA and 2RDA diets. Therefore, in the present study, a twofold difference in dietary protein intake did not alter gut microbiota or VOC indicative of altered protein fermentation.

AB - Diet has a major influence on the composition and metabolic output of the gut microbiome. Higher-protein diets are often recommended for older consumers; however, the effect of high-protein diets on the gut microbiota and faecal volatile organic compounds (VOC) of elderly participants is unknown. The purpose of the study was to establish if the faecal microbiota composition and VOC in older men are different after a diet containing the recommended dietary intake (RDA) of protein compared with a diet containing twice the RDA (2RDA). Healthy males (74·2 (SD·6) years; n 28) were randomised to consume the RDA of protein (0·8 g protein/kg body weight per d) or 2RDA, for 10 weeks. Dietary protein was provided via whole foods rather than supplementation or fortification. The diets were matched for dietary fibre from fruit and vegetables. Faecal samples were collected pre- and post-intervention for microbiota profiling by 16S ribosomal RNA amplicon sequencing and VOC analysis by head space/solid-phase microextraction/GC-MS. After correcting for multiple comparisons, no significant differences in the abundance of faecal microbiota or VOC associated with protein fermentation were evident between the RDA and 2RDA diets. Therefore, in the present study, a twofold difference in dietary protein intake did not alter gut microbiota or VOC indicative of altered protein fermentation.

KW - 16S amplicon sequencing

KW - Fermentation

KW - Microbiome

KW - Recommended dietary intake

KW - Volatile organic compounds

UR - http://www.scopus.com/inward/record.url?scp=85088087404&partnerID=8YFLogxK

U2 - 10.1017/jns.2020.15

DO - 10.1017/jns.2020.15

M3 - Journal article

C2 - 32742642

AN - SCOPUS:85088087404

VL - 9

JO - Journal of Nutritional Science

JF - Journal of Nutritional Science

SN - 2048-6790

M1 - e25

ER -