Comparative nontargeted profiling of metabolic changes in tissues and biofluids in high-fat diet-fed Ossabaw pig
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Comparative nontargeted profiling of metabolic changes in tissues and biofluids in high-fat diet-fed Ossabaw pig. / Hanhineva, Kati; Barri, Thaer; Kolehmainen, Marjukka; Pekkinen, Jenna; Pihlajamäki, Jussi; Vesterbacka, Arto; Solano-Aguilar, Gloria; Mykkänen, Hannu; Dragsted, Lars Ove; Urban, Joseph F; Poutanen, Kaisa.
In: Journal of Proteome Research, Vol. 12, No. 9, 2013, p. 3980-3992.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Comparative nontargeted profiling of metabolic changes in tissues and biofluids in high-fat diet-fed Ossabaw pig
AU - Hanhineva, Kati
AU - Barri, Thaer
AU - Kolehmainen, Marjukka
AU - Pekkinen, Jenna
AU - Pihlajamäki, Jussi
AU - Vesterbacka, Arto
AU - Solano-Aguilar, Gloria
AU - Mykkänen, Hannu
AU - Dragsted, Lars Ove
AU - Urban, Joseph F
AU - Poutanen, Kaisa
N1 - CURIS 2013 NEXS 180
PY - 2013
Y1 - 2013
N2 - Typical clinical biomarker analyses on urine and plasma samples from human dietary interventions do not provide adequate information about diet-induced metabolic changes taking place in tissues. The aim of this study was to show how a large-scale non-targeted metabolomic approach can be used to reveal metabolite groups for generating new hypotheses of obesity-related metabolic disturbances produced in an animal model. A large spectrum of metabolites in the semi-polar region, including small water soluble molecules like betaine and dihydroxyindole, and a wide range of bile acids as well as various lipid species were detected. The high fat diet influenced metabolic homeostasis of Ossabaw pigs, especially the lipid metabolome, throughout all the analyzed sample types, including plasma, urine, bile, liver, pancreas, brain cortex, intestinal jejunum and proximal colon. However, even dramatic metabolic changes in tissues were not necessarily observed in plasma and urine. Metabolite profiling involving multiple sample types was shown to be a feasible method for the examination of a wide spectrum of metabolic species extending from small water soluble metabolites to an array of bile acids and lipids, thus pointing to the pathways of metabolism affected by the dietary treatment.
AB - Typical clinical biomarker analyses on urine and plasma samples from human dietary interventions do not provide adequate information about diet-induced metabolic changes taking place in tissues. The aim of this study was to show how a large-scale non-targeted metabolomic approach can be used to reveal metabolite groups for generating new hypotheses of obesity-related metabolic disturbances produced in an animal model. A large spectrum of metabolites in the semi-polar region, including small water soluble molecules like betaine and dihydroxyindole, and a wide range of bile acids as well as various lipid species were detected. The high fat diet influenced metabolic homeostasis of Ossabaw pigs, especially the lipid metabolome, throughout all the analyzed sample types, including plasma, urine, bile, liver, pancreas, brain cortex, intestinal jejunum and proximal colon. However, even dramatic metabolic changes in tissues were not necessarily observed in plasma and urine. Metabolite profiling involving multiple sample types was shown to be a feasible method for the examination of a wide spectrum of metabolic species extending from small water soluble metabolites to an array of bile acids and lipids, thus pointing to the pathways of metabolism affected by the dietary treatment.
U2 - 10.1021/pr400257d
DO - 10.1021/pr400257d
M3 - Journal article
C2 - 23805856
VL - 12
SP - 3980
EP - 3992
JO - Journal of Proteome Research
JF - Journal of Proteome Research
SN - 1535-3893
IS - 9
ER -
ID: 47082067