We investigated the control of upright standing in individuals with unilateral transtibial amputation (TTA) by assessing the inter-limb coupling and the coupling between the center of pressure beneath both limbs combined (COP_NET) and the center of pressure (COP) beneath the prosthetic limb and the intact limb. Twenty-one adults with TTA and eighteen unimpaired adults completed 90 s of standing on two parallel force plates. The inter-limb coupling and the coupling between the COP beneath each limb and the COP_NET were assessed by quantifying the synchronization of the COP signals. This included the number of epochs with synchronized signals, the total duration of signal synchronization and the relative phase and deviation phase between the signals. Additionally, magnitude and temporal characteristics of the COP displacements were quantified. Individuals with TTA exhibited looser inter-limb coupling in the anterior-posterior direction, characterized by more shifts between epochs with synchronized signals, shorter total duration of signal synchronization, less in-phase coordination patterns and a higher deviation phase between the two limbs, compared to unimpaired individuals. This coincided with a larger and more irregular postural sway in the TTA group. No group difference was observed in the mediolateral direction. The coupling between the COP_NET and the COP beneath the individual limbs was similarly direction dependent, and tighter for the intact side, suggesting that an intact limb-driven strategy was utilized.