Seronegative patients with primary Sjögren's syndrome and non-pSS sicca test positive for anti-SSA/Ro52 and -Ro60 in saliva

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Standard

Seronegative patients with primary Sjögren's syndrome and non-pSS sicca test positive for anti-SSA/Ro52 and -Ro60 in saliva. / Kamounah, Sarah; Wei , Fang; Park, Jin Kyun; Song, Yeong Wook; Chia, David ; Wong, David T. W. ; Pedersen, Anne Marie Lynge.

I: B B A - Molecular Basis of Disease, Bind 1870, Nr. 5, 167168, 2024.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Kamounah, S, Wei , F, Park, JK, Song, YW, Chia, D, Wong, DTW & Pedersen, AML 2024, 'Seronegative patients with primary Sjögren's syndrome and non-pSS sicca test positive for anti-SSA/Ro52 and -Ro60 in saliva', B B A - Molecular Basis of Disease, bind 1870, nr. 5, 167168. https://doi.org/10.1016/j.bbadis.2024.167168

APA

Kamounah, S., Wei , F., Park, J. K., Song, Y. W., Chia, D., Wong, D. T. W., & Pedersen, A. M. L. (2024). Seronegative patients with primary Sjögren's syndrome and non-pSS sicca test positive for anti-SSA/Ro52 and -Ro60 in saliva. B B A - Molecular Basis of Disease, 1870(5), [167168]. https://doi.org/10.1016/j.bbadis.2024.167168

Vancouver

Kamounah S, Wei F, Park JK, Song YW, Chia D, Wong DTW o.a. Seronegative patients with primary Sjögren's syndrome and non-pSS sicca test positive for anti-SSA/Ro52 and -Ro60 in saliva. B B A - Molecular Basis of Disease. 2024;1870(5). 167168. https://doi.org/10.1016/j.bbadis.2024.167168

Author

Kamounah, Sarah ; Wei , Fang ; Park, Jin Kyun ; Song, Yeong Wook ; Chia, David ; Wong, David T. W. ; Pedersen, Anne Marie Lynge. / Seronegative patients with primary Sjögren's syndrome and non-pSS sicca test positive for anti-SSA/Ro52 and -Ro60 in saliva. I: B B A - Molecular Basis of Disease. 2024 ; Bind 1870, Nr. 5.

Bibtex

@article{27dc1fdb12c9434195530c8042b2be26,
title = "Seronegative patients with primary Sj{\"o}gren's syndrome and non-pSS sicca test positive for anti-SSA/Ro52 and -Ro60 in saliva",
abstract = "Objectives: Testing for anti-SSA/Ro antibodies in serum is essential in the diagnostic work-up for primarySjogren ¨ {\textquoteright}s syndrome (pSS). In this study, we aimed to validate our previous assay for detection of salivary antiSSA/Ro52, and to develop assays for detection of salivary anti-SSA/Ro60 and for detection of anti-Ro52 and-Ro60 in plasma using the electric field-induced release and measurement (EFIRM) platform.Methods: Whole saliva samples from two independent Danish cohorts (DN1 and DN2) including 49 patients withpSS, 73 patients with sicca symptoms, but not fulfilling the classification criteria for pSS (non-pSS sicca), and 51healthy controls (HC), as well as plasma samples from the DN1 cohort were analyzed using EFIRM to detect antiSSA/Ro52 and -Ro60.Results: In the DN1 cohort, 100 % in the pSS group and 16 % in the non-pSS sicca group were serum anti-SSA/Ropositive by ELISA. EFIRM detected anti-SSA (Ro52 and/or -Ro60) in plasma and saliva in 100 % and 96 %patients with pSS, and 16 % and 29 % with non-pSS sicca. In the DN2 cohort, 80 % patients with pSS and 26 %with non-pSS sicca were serum anti-SSA/Ro positive. Salivary anti-SSA discriminated patients with pSS from HCand non-pSS sicca with an AUC range of 0.74–0.96 in the DN1 and DN2 cohorts. EFIRM discriminated pSS fromnon-pSS sicca with an AUC of 0.98 in plasma.Conclusion: Our findings suggest that salivary anti-SSA/Ro antibodies are potential discriminatory biomarkers forpSS, which may also identify seronegative patients, addressing the unmet clinical need of early detection andstratification of pSS. ",
author = "Sarah Kamounah and Fang Wei and Park, {Jin Kyun} and Song, {Yeong Wook} and David Chia and Wong, {David T. W.} and Pedersen, {Anne Marie Lynge}",
year = "2024",
doi = "10.1016/j.bbadis.2024.167168",
language = "English",
volume = "1870",
journal = "B B A - Molecular Basis of Disease",
issn = "0925-4439",
publisher = "Elsevier",
number = "5",

}

RIS

TY - JOUR

T1 - Seronegative patients with primary Sjögren's syndrome and non-pSS sicca test positive for anti-SSA/Ro52 and -Ro60 in saliva

AU - Kamounah, Sarah

AU - Wei , Fang

AU - Park, Jin Kyun

AU - Song, Yeong Wook

AU - Chia, David

AU - Wong, David T. W.

AU - Pedersen, Anne Marie Lynge

PY - 2024

Y1 - 2024

N2 - Objectives: Testing for anti-SSA/Ro antibodies in serum is essential in the diagnostic work-up for primarySjogren ¨ ’s syndrome (pSS). In this study, we aimed to validate our previous assay for detection of salivary antiSSA/Ro52, and to develop assays for detection of salivary anti-SSA/Ro60 and for detection of anti-Ro52 and-Ro60 in plasma using the electric field-induced release and measurement (EFIRM) platform.Methods: Whole saliva samples from two independent Danish cohorts (DN1 and DN2) including 49 patients withpSS, 73 patients with sicca symptoms, but not fulfilling the classification criteria for pSS (non-pSS sicca), and 51healthy controls (HC), as well as plasma samples from the DN1 cohort were analyzed using EFIRM to detect antiSSA/Ro52 and -Ro60.Results: In the DN1 cohort, 100 % in the pSS group and 16 % in the non-pSS sicca group were serum anti-SSA/Ropositive by ELISA. EFIRM detected anti-SSA (Ro52 and/or -Ro60) in plasma and saliva in 100 % and 96 %patients with pSS, and 16 % and 29 % with non-pSS sicca. In the DN2 cohort, 80 % patients with pSS and 26 %with non-pSS sicca were serum anti-SSA/Ro positive. Salivary anti-SSA discriminated patients with pSS from HCand non-pSS sicca with an AUC range of 0.74–0.96 in the DN1 and DN2 cohorts. EFIRM discriminated pSS fromnon-pSS sicca with an AUC of 0.98 in plasma.Conclusion: Our findings suggest that salivary anti-SSA/Ro antibodies are potential discriminatory biomarkers forpSS, which may also identify seronegative patients, addressing the unmet clinical need of early detection andstratification of pSS.

AB - Objectives: Testing for anti-SSA/Ro antibodies in serum is essential in the diagnostic work-up for primarySjogren ¨ ’s syndrome (pSS). In this study, we aimed to validate our previous assay for detection of salivary antiSSA/Ro52, and to develop assays for detection of salivary anti-SSA/Ro60 and for detection of anti-Ro52 and-Ro60 in plasma using the electric field-induced release and measurement (EFIRM) platform.Methods: Whole saliva samples from two independent Danish cohorts (DN1 and DN2) including 49 patients withpSS, 73 patients with sicca symptoms, but not fulfilling the classification criteria for pSS (non-pSS sicca), and 51healthy controls (HC), as well as plasma samples from the DN1 cohort were analyzed using EFIRM to detect antiSSA/Ro52 and -Ro60.Results: In the DN1 cohort, 100 % in the pSS group and 16 % in the non-pSS sicca group were serum anti-SSA/Ropositive by ELISA. EFIRM detected anti-SSA (Ro52 and/or -Ro60) in plasma and saliva in 100 % and 96 %patients with pSS, and 16 % and 29 % with non-pSS sicca. In the DN2 cohort, 80 % patients with pSS and 26 %with non-pSS sicca were serum anti-SSA/Ro positive. Salivary anti-SSA discriminated patients with pSS from HCand non-pSS sicca with an AUC range of 0.74–0.96 in the DN1 and DN2 cohorts. EFIRM discriminated pSS fromnon-pSS sicca with an AUC of 0.98 in plasma.Conclusion: Our findings suggest that salivary anti-SSA/Ro antibodies are potential discriminatory biomarkers forpSS, which may also identify seronegative patients, addressing the unmet clinical need of early detection andstratification of pSS.

U2 - 10.1016/j.bbadis.2024.167168

DO - 10.1016/j.bbadis.2024.167168

M3 - Journal article

C2 - 38641012

VL - 1870

JO - B B A - Molecular Basis of Disease

JF - B B A - Molecular Basis of Disease

SN - 0925-4439

IS - 5

M1 - 167168

ER -

ID: 388282372