Laser-assisted microdissection of membrane-mounted paraffin sections for polymerase chain reaction analysis: Identification of cell populations using immunohistochemistry and in situ hybridization

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Standard

Laser-assisted microdissection of membrane-mounted paraffin sections for polymerase chain reaction analysis : Identification of cell populations using immunohistochemistry and in situ hybridization. / Gjerdrum, Lise Mette; Lielpetere, Ilze; Rasmussen, Lars Melholt; Bendix, Knud; Hamilton-Dutoit, Stephen.

I: Journal of Molecular Diagnostics, Bind 3, Nr. 3, 60659, 2001, s. 105-110.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Gjerdrum, LM, Lielpetere, I, Rasmussen, LM, Bendix, K & Hamilton-Dutoit, S 2001, 'Laser-assisted microdissection of membrane-mounted paraffin sections for polymerase chain reaction analysis: Identification of cell populations using immunohistochemistry and in situ hybridization', Journal of Molecular Diagnostics, bind 3, nr. 3, 60659, s. 105-110. https://doi.org/10.1016/S1525-1578(10)60659-9

APA

Gjerdrum, L. M., Lielpetere, I., Rasmussen, L. M., Bendix, K., & Hamilton-Dutoit, S. (2001). Laser-assisted microdissection of membrane-mounted paraffin sections for polymerase chain reaction analysis: Identification of cell populations using immunohistochemistry and in situ hybridization. Journal of Molecular Diagnostics, 3(3), 105-110. [60659]. https://doi.org/10.1016/S1525-1578(10)60659-9

Vancouver

Gjerdrum LM, Lielpetere I, Rasmussen LM, Bendix K, Hamilton-Dutoit S. Laser-assisted microdissection of membrane-mounted paraffin sections for polymerase chain reaction analysis: Identification of cell populations using immunohistochemistry and in situ hybridization. Journal of Molecular Diagnostics. 2001;3(3):105-110. 60659. https://doi.org/10.1016/S1525-1578(10)60659-9

Author

Gjerdrum, Lise Mette ; Lielpetere, Ilze ; Rasmussen, Lars Melholt ; Bendix, Knud ; Hamilton-Dutoit, Stephen. / Laser-assisted microdissection of membrane-mounted paraffin sections for polymerase chain reaction analysis : Identification of cell populations using immunohistochemistry and in situ hybridization. I: Journal of Molecular Diagnostics. 2001 ; Bind 3, Nr. 3. s. 105-110.

Bibtex

@article{02dfd9029a1d453f8ad7ea2da0323b66,
title = "Laser-assisted microdissection of membrane-mounted paraffin sections for polymerase chain reaction analysis: Identification of cell populations using immunohistochemistry and in situ hybridization",
abstract = "Laser microbeam microdissection (LMM) is an increasingly important method for obtaining pure cell samples for genetic and proteomic analysis. Immuno-histochemistry (IHC) and in situ hybridization (ISH) are useful techniques for targeting specific cell populations for microdissection but are difficult to apply with the tissue support membranes often used during LMM. Using detection of cytokeratins and Epstein-Barr virus gene products in head and neck carcinoma as a model, we describe optimized protocols for membrane and section preparation and for low temperature antigen retrieval that allow IHC and ISH to be used reliably on membrane mounted paraffin tissue sections. Visualization of cellular targets was markedly improved by staining and this could be further improved using a variety of optical media before microdissection. Tissue fragments thus stained were suitable for subsequent polymerase chain reaction analysis of extracted DNA using standard techniques. These IHC and ISH procedures are generally applicable and will be useful for detecting a wide range of antigens and nucleic acids in paraffin sections in conjunction with LMM.",
author = "Gjerdrum, {Lise Mette} and Ilze Lielpetere and Rasmussen, {Lars Melholt} and Knud Bendix and Stephen Hamilton-Dutoit",
note = "Funding Information: Supported by Aarhus University Research Foundation; King Christian X's Foundation; Eva and Henry Fr{\ae}nels' Foundation; the Leo Foundation; the Novo Nordisk Foundation; Else and Mogens Wedell-Wedellsborg Foundation; Einar Willumsen Memorial Foundation; Max and Inger W{\o}rzners Memorial Foundation. ",
year = "2001",
doi = "10.1016/S1525-1578(10)60659-9",
language = "English",
volume = "3",
pages = "105--110",
journal = "Journal of Molecular Diagnostics",
issn = "1525-1578",
publisher = "Elsevier",
number = "3",

}

RIS

TY - JOUR

T1 - Laser-assisted microdissection of membrane-mounted paraffin sections for polymerase chain reaction analysis

T2 - Identification of cell populations using immunohistochemistry and in situ hybridization

AU - Gjerdrum, Lise Mette

AU - Lielpetere, Ilze

AU - Rasmussen, Lars Melholt

AU - Bendix, Knud

AU - Hamilton-Dutoit, Stephen

N1 - Funding Information: Supported by Aarhus University Research Foundation; King Christian X's Foundation; Eva and Henry Frænels' Foundation; the Leo Foundation; the Novo Nordisk Foundation; Else and Mogens Wedell-Wedellsborg Foundation; Einar Willumsen Memorial Foundation; Max and Inger Wørzners Memorial Foundation.

PY - 2001

Y1 - 2001

N2 - Laser microbeam microdissection (LMM) is an increasingly important method for obtaining pure cell samples for genetic and proteomic analysis. Immuno-histochemistry (IHC) and in situ hybridization (ISH) are useful techniques for targeting specific cell populations for microdissection but are difficult to apply with the tissue support membranes often used during LMM. Using detection of cytokeratins and Epstein-Barr virus gene products in head and neck carcinoma as a model, we describe optimized protocols for membrane and section preparation and for low temperature antigen retrieval that allow IHC and ISH to be used reliably on membrane mounted paraffin tissue sections. Visualization of cellular targets was markedly improved by staining and this could be further improved using a variety of optical media before microdissection. Tissue fragments thus stained were suitable for subsequent polymerase chain reaction analysis of extracted DNA using standard techniques. These IHC and ISH procedures are generally applicable and will be useful for detecting a wide range of antigens and nucleic acids in paraffin sections in conjunction with LMM.

AB - Laser microbeam microdissection (LMM) is an increasingly important method for obtaining pure cell samples for genetic and proteomic analysis. Immuno-histochemistry (IHC) and in situ hybridization (ISH) are useful techniques for targeting specific cell populations for microdissection but are difficult to apply with the tissue support membranes often used during LMM. Using detection of cytokeratins and Epstein-Barr virus gene products in head and neck carcinoma as a model, we describe optimized protocols for membrane and section preparation and for low temperature antigen retrieval that allow IHC and ISH to be used reliably on membrane mounted paraffin tissue sections. Visualization of cellular targets was markedly improved by staining and this could be further improved using a variety of optical media before microdissection. Tissue fragments thus stained were suitable for subsequent polymerase chain reaction analysis of extracted DNA using standard techniques. These IHC and ISH procedures are generally applicable and will be useful for detecting a wide range of antigens and nucleic acids in paraffin sections in conjunction with LMM.

UR - http://www.scopus.com/inward/record.url?scp=0034893965&partnerID=8YFLogxK

U2 - 10.1016/S1525-1578(10)60659-9

DO - 10.1016/S1525-1578(10)60659-9

M3 - Journal article

C2 - 11486049

AN - SCOPUS:0034893965

VL - 3

SP - 105

EP - 110

JO - Journal of Molecular Diagnostics

JF - Journal of Molecular Diagnostics

SN - 1525-1578

IS - 3

M1 - 60659

ER -

ID: 375144970