TY - JOUR

T1 - Interaction between prostaglandins of the E-type with a urinary component from halothane anesthetized rats.

AU - Christensen, P

AU - Holstein-Rathlou, N H

N1 - Keywords: Anesthetics; Animals; Chromatography, Gel; Chromatography, Thin Layer; Dinoprost; Dinoprostone; Halothane; Hexobarbital; Male; Nitrous Oxide; Prostaglandins A; Prostaglandins E; Prostaglandins F; Radioimmunoassay; Rats; Rats, Inbred Strains

PY - 1981

Y1 - 1981

N2 - Prostaglandins of the E-type (PGE's) were found to react or combine with a urinary metabolite of Halothane yielding products which were left unrecovered during the purification procedure preceding specific radioimmunoassay of PGE2. The products were retained on sephadex LH-20 columns, and showed on thin layer silica gel plates (TLC) Rf values lower than those of the parent PGE-compounds. The product formation is supposed to involve the beta-hydroxyketone system of PGE, since PG's of the F and A type were unaffected. The product formation could be avoided by inducing anaesthesia with Hexobarbitone and maintaining the anaesthesia with Halothane-nitrous oxide or it could be reversed by adding barbiturates to urine samples obtained from animals anaesthetized with Halothane-nitrous oxide alone. The barbiturates effectively competed with PGE for the metabolite leaving PGE to behave normally on sephadex LH-20 and TLC, thus enabling us to evaluate correctly the PGE2 content by RIA.

AB - Prostaglandins of the E-type (PGE's) were found to react or combine with a urinary metabolite of Halothane yielding products which were left unrecovered during the purification procedure preceding specific radioimmunoassay of PGE2. The products were retained on sephadex LH-20 columns, and showed on thin layer silica gel plates (TLC) Rf values lower than those of the parent PGE-compounds. The product formation is supposed to involve the beta-hydroxyketone system of PGE, since PG's of the F and A type were unaffected. The product formation could be avoided by inducing anaesthesia with Hexobarbitone and maintaining the anaesthesia with Halothane-nitrous oxide or it could be reversed by adding barbiturates to urine samples obtained from animals anaesthetized with Halothane-nitrous oxide alone. The barbiturates effectively competed with PGE for the metabolite leaving PGE to behave normally on sephadex LH-20 and TLC, thus enabling us to evaluate correctly the PGE2 content by RIA.

M3 - Journal article

C2 - 6950460

VL - 22

SP - 893

EP - 902

JO - Prostaglandins and Other Lipid Mediators

JF - Prostaglandins and Other Lipid Mediators

SN - 1098-8823

IS - 6

ER -