3D organoid assay of the impact of infected erythrocyte adhesion on the blood-brain barrier
Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
Mass sequestration of Plasmodium falciparum parasites in the brain microvasculature can lead to cerebral malaria (CM), characterized by inflammation, vessel occlusion, and brain swelling. To date, only single-cell-type, monolayer assays have been used to investigate the effect of infected erythrocytes (IEs) on the human blood-brain barrier (BBB) and the underlying parenchyma. Here we present a human-derived 3D model of the BBB comprised of endothelial cells, pericytes, and astrocytes in direct contact with each other. The organoids readily self-assemble and can easily be grown in 96-well plates, allowing for high-throughput analysis. These organoids allow for the assessment of parasite adhesion, and analysis of barrier function, and gross morphological changes in response to parasite exposure.
Originalsprog | Engelsk |
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Titel | Malaria Immunology : Targeting the Surface of Infected Erythrocytes |
Antal sider | 13 |
Vol/bind | 2470 |
Forlag | Humana Press |
Publikationsdato | 2022 |
Sider | 587-599 |
ISBN (Elektronisk) | 978-1-0716-2189-9 |
DOI | |
Status | Udgivet - 2022 |
Navn | Methods in molecular biology (Clifton, N.J.) |
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ISSN | 1064-3745 |
Bibliografisk note
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
ID: 320648836