3D Analysis of Synaptic Ultrastructure in Organotypic Hippocampal Slice Culture by High-Pressure Freezing and Electron Tomography
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning
Transmission electron microscopy serves as a valuable tool for synaptic structure-function analyses aimed at identifying morphological features or modifications associated with specific developmental stages or dysfunctional synaptic states. By utilizing cryo-preparation techniques to minimize the introduction of structural artifacts during sample preparation, and electron tomography to reconstruct the 3D ultrastructural architecture of a synapse, the spatial organization and morphological properties of synaptic organelles and subcompartments can be quantified with unparalleled precision. In this chapter, we present an experimental approach combining organotypic slice culture, high-pressure freezing, automated freeze-substitution, and electron tomography to investigate spatial relationships between synaptic vesicles and active zone release sites in synapses from lethal mouse mutants.
Originalsprog | Engelsk |
---|---|
Tidsskrift | Methods in molecular biology (Clifton, N.J.) |
Vol/bind | 1538 |
Sider (fra-til) | 215-231 |
Antal sider | 17 |
ISSN | 1064-3745 |
DOI | |
Status | Udgivet - 2017 |
Eksternt udgivet | Ja |
ID: 237697866