2.4-Å structure of the double-ring Gemmatimonas phototrophica photosystem

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  • Pu Qian
  • Alastair T. Gardiner
  • Ivana Šímová
  • Katerina Naydenova
  • Tristan I. Croll
  • Philip J. Jackson
  • Nupur
  • Miroslav Kloz
  • Petra Čubáková
  • Marek Kuzma
  • Yonghui Zeng
  • Pablo Castro-Hartmann
  • Bart van Knippenberg
  • Kenneth N. Goldie
  • David Kaftan
  • Pavel Hrouzek
  • Jan Hájek
  • Jon Agirre
  • C. Alistair Siebert
  • David Bína
  • Og 7 flere
  • Kasim Sader
  • Henning Stahlberg
  • Roman Sobotka
  • Christopher J. Russo
  • Tomáš Polívka
  • C. Neil Hunter
  • Michal Koblížek

Phototrophic Gemmatimonadetes evolved the ability to use solar energy following horizontal transfer of photosynthesis-related genes from an ancient phototrophic proteobacterium. The electron cryo-microscopy structure of the Gemmatimonas phototrophica photosystem at 2.4 Å reveals a unique, double-ring complex. Two unique membrane-extrinsic polypeptides, RC-S and RC-U, hold the central type 2 reaction center (RC) within an inner 16-subunit light-harvesting 1 (LH1) ring, which is encircled by an outer 24-subunit antenna ring (LHh) that adds light-gathering capacity. Femtosecond kinetics reveal the flow of energy within the RC-dLH complex, from the outer LHh ring to LH1 and then to the RC. This structural and functional study shows that G. phototrophica has independently evolved its own compact, robust, and highly effective architecture for harvesting and trapping solar energy.

OriginalsprogEngelsk
Artikelnummereabk3139
TidsskriftScience Advances
Vol/bind8
Udgave nummer7
Antal sider12
ISSN2375-2548
DOI
StatusUdgivet - 2022

Bibliografisk note

Funding Information:
This study was mainly financed by the Czech Science Foundation, EXPRO project 19-28778X PhotoGemm+ (M.Ko.); Czech Science Foundation, EXPRO project 19-28323X (T.P.); Biotechnology and Biological Sciences Research Council (BBSRC) UK, award number BB/M000265/1 (P.Q. and C.N.H.); European Research Council Synergy Award 854126 (C.N.H.); European Regional Development Fund Project ?Mechanisms and dynamics of macromolecular complexes: From single molecules to cells? CZ.02.1.01/0.0/0.0/15_003/0000441 (D.K.); Medical Research Council Grant MC_UP_120117 (C.J.R.); Sir John Bradfield Scholarship from the Cambridge Trust (K.N.); Royal Society Olga Kennard Research Fellow, UF160039 (J.A.); and Wellcome Trust grant 209407/Z/17/Z (T.I.C.).

Publisher Copyright:
Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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