In the present study, a novel intermittent badminton endurance test (B-ENDURANCE) was developed and tested in elite (n=17) and skilled (n=9) badminton players as well as in age-matched physically active men (non-badminton players; n=8). In addition, B-ENDURANCE test-retest reproducibility was evaluated in nine badminton players.B-ENDURANCE is an incremental test where each level consists of repeated sequences of badminton specific actions towards the four corners on the court. The subject starts in the center of the court in front of a computer screen and within each sequence he must in a randomized order complete eight actions as dictated by the computer providing the audiovisual input and verifying that the appropriate sensor is activated within the allocated time. Recovery time between each sequence is 10 seconds throughout the test, but the time to complete each sequence is gradually decreases until the subjects cannot follow the dictated tempo.B-ENDURANCE performance for elite players was better (P<0.05) compared to the skilled players and non-badminton players. In addition, B-ENDURANCE performance correlated (r=0.8; P<0.0001) with elite players' national single ranking. Test-retest coefficient of variation was 7.9 % between the first two trials (i.e. without a familiarization trial), but reduced to 2.5 % when comparing the 2 and 3 trial. In conclusion, the B-ENDURANCE test is relevant for evaluation of badminton specific endurance, but at least one familiarization trial is recommended if the test is used for evaluation of longitudinal changes, e.g. tracking training effects.