Heat shock factor activation in human muscles following a demanding intermittent exercise protocol is attenuated with hyperthermia
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Heat shock factor activation in human muscles following a demanding intermittent exercise protocol is attenuated with hyperthermia. / Palomero, J; Broome, C S; Rasmussen, P; Mohr, Magni; Nielsen, B; Nybo, Lars; McArdle, A; Drust, B.
I: Acta Physiologica (Print Edition), Bind 193, Nr. 1, 2008, s. 79-88.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Heat shock factor activation in human muscles following a demanding intermittent exercise protocol is attenuated with hyperthermia
AU - Palomero, J
AU - Broome, C S
AU - Rasmussen, P
AU - Mohr, Magni
AU - Nielsen, B
AU - Nybo, Lars
AU - McArdle, A
AU - Drust, B
N1 - CURIS 2008 5200 031
PY - 2008
Y1 - 2008
N2 - AIM: The present study investigated whether increased activation of heat shock factors (HSF) following exercise relates primarily to the increased muscle temperature or to exercise in general. METHODS: Six subjects completed 40 min of intermittent cycling (15s:15s exercise:recovery at 300 +/- 22 W) at an ambient temperature of either 20.0 +/- 1.3 or 40.3 +/- 0.7 degrees C. Muscle biopsies were taken prior to and immediately following the exercise protocol with samples analysed for HSF DNA binding by electrophoretic mobility shift assay. RESULTS: Exercise at 40 degrees C resulted in significantly increased oesophageal (39.3 +/- 0.2 degrees C) and muscle temperature (40.0 +/- 0.2 degrees C) at the end of the exercise protocol compared with 20 degrees C (oesophageal, 38.1 +/- 0.1 degrees C; muscle, 38.9 +/- 0.2 degrees C). However, an increased DNA binding of HSF was not evident following exercise at 40 degrees C (reduced by 21 +/- 22%) whereas it increased by 29 +/- 51% following exercise at 20 degrees C. CONCLUSION: It appears that increased temperature is not the major factor responsible for activation of HSF DNA binding.
AB - AIM: The present study investigated whether increased activation of heat shock factors (HSF) following exercise relates primarily to the increased muscle temperature or to exercise in general. METHODS: Six subjects completed 40 min of intermittent cycling (15s:15s exercise:recovery at 300 +/- 22 W) at an ambient temperature of either 20.0 +/- 1.3 or 40.3 +/- 0.7 degrees C. Muscle biopsies were taken prior to and immediately following the exercise protocol with samples analysed for HSF DNA binding by electrophoretic mobility shift assay. RESULTS: Exercise at 40 degrees C resulted in significantly increased oesophageal (39.3 +/- 0.2 degrees C) and muscle temperature (40.0 +/- 0.2 degrees C) at the end of the exercise protocol compared with 20 degrees C (oesophageal, 38.1 +/- 0.1 degrees C; muscle, 38.9 +/- 0.2 degrees C). However, an increased DNA binding of HSF was not evident following exercise at 40 degrees C (reduced by 21 +/- 22%) whereas it increased by 29 +/- 51% following exercise at 20 degrees C. CONCLUSION: It appears that increased temperature is not the major factor responsible for activation of HSF DNA binding.
U2 - 10.1111/j.1748-1716.2007.01774.x
DO - 10.1111/j.1748-1716.2007.01774.x
M3 - Journal article
C2 - 18081888
VL - 193
SP - 79
EP - 88
JO - Acta Physiologica
JF - Acta Physiologica
SN - 1748-1708
IS - 1
ER -
ID: 3592277