Glucose turnover during insulin-induced hypoglycemia in liver-denervated rats
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Glucose turnover during insulin-induced hypoglycemia in liver-denervated rats. / Mikines, K J; Sonne, B; Richter, Erik A.; Christensen, N J; Galbo, H.
In: American Journal of Physiology (Consolidated), Vol. 248, No. 3 Pt 1, 1985, p. E327-E332.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Glucose turnover during insulin-induced hypoglycemia in liver-denervated rats
AU - Mikines, K J
AU - Sonne, B
AU - Richter, Erik A.
AU - Christensen, N J
AU - Galbo, H
PY - 1985
Y1 - 1985
N2 - The role of hepatic autonomic nerves in glucose production during hypoglycemia was studied. Selective, surgical denervation of the liver was performed in rats, which reduced hepatic norepinephrine concentrations by 96%. Hypoglycemia was induced by 250 mU of insulin intra-arterially in anesthetized as well as in chronically catheterized, awake rats. Half of the anesthetized denervated or sham-operated rats had previously been adrenodemedullated. Glucose turnover was measured by primed, constant intravenous infusion of [3-3H]glucose. Before as well as during hypoglycemia the arterial glucose concentration and rates of production and utilization of glucose were similar in denervated rats and control rats. Also hepatic glycogen depletion was similar in the groups. The lack of effect of denervation could not be ascribed to compensating changes in hormone or substrate levels. In adrenodemedullated rats lack of glucose recovery from hypoglycemia was accompanied by delayed normalization of glucose clearance. In fed rats, activity in hepatic autonomic nerves is not a primary mechanism increasing glucose production during acute hypoglycemia. Epinephrine enhances glucose recovery by decreasing glucose clearance rather than by increasing glucose production, at least when glucagon is present.
AB - The role of hepatic autonomic nerves in glucose production during hypoglycemia was studied. Selective, surgical denervation of the liver was performed in rats, which reduced hepatic norepinephrine concentrations by 96%. Hypoglycemia was induced by 250 mU of insulin intra-arterially in anesthetized as well as in chronically catheterized, awake rats. Half of the anesthetized denervated or sham-operated rats had previously been adrenodemedullated. Glucose turnover was measured by primed, constant intravenous infusion of [3-3H]glucose. Before as well as during hypoglycemia the arterial glucose concentration and rates of production and utilization of glucose were similar in denervated rats and control rats. Also hepatic glycogen depletion was similar in the groups. The lack of effect of denervation could not be ascribed to compensating changes in hormone or substrate levels. In adrenodemedullated rats lack of glucose recovery from hypoglycemia was accompanied by delayed normalization of glucose clearance. In fed rats, activity in hepatic autonomic nerves is not a primary mechanism increasing glucose production during acute hypoglycemia. Epinephrine enhances glucose recovery by decreasing glucose clearance rather than by increasing glucose production, at least when glucagon is present.
KW - Adrenal Cortex Hormones
KW - Adrenal Medulla
KW - Animals
KW - Autonomic Nervous System
KW - Blood Glucose
KW - Denervation
KW - Epinephrine
KW - Gluconeogenesis
KW - Glucose
KW - Hypoglycemia
KW - Insulin
KW - Liver
KW - Male
KW - Rats
KW - Rats, Inbred Strains
M3 - Journal article
C2 - 3883803
VL - 248
SP - E327-E332
JO - American Journal of Physiology - Cell Physiology
JF - American Journal of Physiology - Cell Physiology
SN - 0363-6143
IS - 3 Pt 1
ER -
ID: 154758878