TY - JOUR

T1 - Effect of marked weight loss on adiponectin gene expression and plasma concentrations

AU - Coughlin, Carrie C

AU - Finck, Brian N

AU - Eagon, J Christopher

AU - Halpin, Valerie J

AU - Magkos, Faidon

AU - Mohammed, B Selma

AU - Klein, Samuel

N1 - (Ekstern)

PY - 2007

Y1 - 2007

N2 - Objective: Adiponectin is the most abundant protein secreted by adipose tissue and is inversely associated with adiposity and insulin resistance. The aim of this study was to evaluate the hypothesis that marked weight loss, induced by gastric bypass surgery (GBS), would increase adiponectin gene expression in both upper and lower subcutaneous body fat and increase plasma adiponectin concentration.Research methods and procedures: Adiponectin subcutaneous abdominal and femoral adipose tissue gene expression, determined by using quantitative reverse transcriptase-polymerase chain reaction, and adiponectin plasma concentrations, determined by using enzyme-linked immunosorbent assay, were evaluated in six extremely obese women (BMI = 57.1 +/- 4.1 kg/m2) before and 1 year after GBS.Results: After GBS, subjects lost 36 +/- 5% of their initial body weight and showed increased insulin sensitivity, manifested by a marked decrease in homeostasis model assessment of insulin resistance from 10.7 +/- 4.1 to 1.4 +/- 0.3 (p < 0.05). Adiponectin gene expression increased 8- to 15-fold in subcutaneous abdominal and femoral adipose tissues (p < 0.05). Plasma adiponectin concentrations increased from 5.2 +/- 0.9 to 8.4 +/- 1.2 mug/mL (p < 0.05).Discussion: These data show that marked weight loss induced by GBS increases adiponectin gene expression in both upper- and lower-body subcutaneous fat. The increase in adipose tissue adiponectin production resulted in an increase in plasma adiponectin concentrations, which likely contributed to the decrease in insulin resistance observed after weight loss.

AB - Objective: Adiponectin is the most abundant protein secreted by adipose tissue and is inversely associated with adiposity and insulin resistance. The aim of this study was to evaluate the hypothesis that marked weight loss, induced by gastric bypass surgery (GBS), would increase adiponectin gene expression in both upper and lower subcutaneous body fat and increase plasma adiponectin concentration.Research methods and procedures: Adiponectin subcutaneous abdominal and femoral adipose tissue gene expression, determined by using quantitative reverse transcriptase-polymerase chain reaction, and adiponectin plasma concentrations, determined by using enzyme-linked immunosorbent assay, were evaluated in six extremely obese women (BMI = 57.1 +/- 4.1 kg/m2) before and 1 year after GBS.Results: After GBS, subjects lost 36 +/- 5% of their initial body weight and showed increased insulin sensitivity, manifested by a marked decrease in homeostasis model assessment of insulin resistance from 10.7 +/- 4.1 to 1.4 +/- 0.3 (p < 0.05). Adiponectin gene expression increased 8- to 15-fold in subcutaneous abdominal and femoral adipose tissues (p < 0.05). Plasma adiponectin concentrations increased from 5.2 +/- 0.9 to 8.4 +/- 1.2 mug/mL (p < 0.05).Discussion: These data show that marked weight loss induced by GBS increases adiponectin gene expression in both upper- and lower-body subcutaneous fat. The increase in adipose tissue adiponectin production resulted in an increase in plasma adiponectin concentrations, which likely contributed to the decrease in insulin resistance observed after weight loss.

KW - Adiponectin/blood

KW - Adult

KW - Body Weight

KW - Female

KW - Gastric Bypass

KW - Gene Expression

KW - Humans

KW - Insulin/blood

KW - Obesity, Morbid/surgery

KW - Weight Loss/physiology

U2 - 10.1038/oby.2007.556

DO - 10.1038/oby.2007.556

M3 - Journal article

C2 - 17372314

VL - 15

SP - 640

EP - 645

JO - Obesity

JF - Obesity

SN - 1930-7381

IS - 3

ER -