Dietary walnuts inhibit colorectal cancer growth in mice by suppressing angiogenesis
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Dietary walnuts inhibit colorectal cancer growth in mice by suppressing angiogenesis. / Nagel, Jutta M; Brinkoetter, Mary; Magkos, Faidon; Liu, Xiaowen; Chamberland, John P; Shah, Sunali; Zhou, Jinrong; Blackburn, George; Mantzoros, Christos S.
I: Nutrition, Bind 28, Nr. 1, 2012, s. 67-75.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Dietary walnuts inhibit colorectal cancer growth in mice by suppressing angiogenesis
AU - Nagel, Jutta M
AU - Brinkoetter, Mary
AU - Magkos, Faidon
AU - Liu, Xiaowen
AU - Chamberland, John P
AU - Shah, Sunali
AU - Zhou, Jinrong
AU - Blackburn, George
AU - Mantzoros, Christos S
N1 - Published by Elsevier Inc.
PY - 2012
Y1 - 2012
N2 - Objective: Animal studies have demonstrated that dietary supplementation with flaxseed oil inhibits colorectal cancer growth. Recent data indicate that walnuts have strong antiproliferative properties against colon cancer cells in vitro but no previous study has assessed the effects of walnuts in vivo or performed a joint evaluation of flaxseed oil and walnuts. The aim of the present study was to examine the effect of dietary walnuts on colorectal cancer in vivo and to comparatively evaluate their efficacy in relation to flaxseed oil.Methods: HT-29 human colon cancer cells were injected in 6-wk-old female nude mice. After a 1-wk acclimation period, mice (n = 48) were randomized to diets containing ∼19% of total energy from walnuts, flaxseed oil, or corn oil (control) and were subsequently studied for 25 d.Results: Tumor growth rate was significantly slower in walnut-fed and flaxseed-fed mice compared with corn oil-fed animals (P < 0.05) by 27% and 43%, respectively. Accordingly, final tumor weight was reduced by 33% and 44%, respectively (P < 0.05 versus control); the differences between walnut and flaxseed diets did not reach significance. We found no differences among groups in metabolic and hormonal profile, serum antioxidant capacity, or inflammation (P > 0.05). However, walnuts and flaxseed oil significantly reduced serum expression levels of angiogenesis factors, including vascular endothelial growth factor (by 30% and 80%, respectively), and approximately doubled total necrotic areas despite smaller tumor sizes (P < 0.05 versus control). Dietary walnuts significantly decreased angiogenesis (CD34 staining; P = 0.017 versus control), whereas this effect did not reach significance in the flaxseed oil group (P = 0.454 versus control).Conclusion: We conclude that walnuts in the diet inhibit colorectal cancer growth by suppressing angiogenesis. Further studies are needed to confirm our findings in humans and explore underlying mechanisms.
AB - Objective: Animal studies have demonstrated that dietary supplementation with flaxseed oil inhibits colorectal cancer growth. Recent data indicate that walnuts have strong antiproliferative properties against colon cancer cells in vitro but no previous study has assessed the effects of walnuts in vivo or performed a joint evaluation of flaxseed oil and walnuts. The aim of the present study was to examine the effect of dietary walnuts on colorectal cancer in vivo and to comparatively evaluate their efficacy in relation to flaxseed oil.Methods: HT-29 human colon cancer cells were injected in 6-wk-old female nude mice. After a 1-wk acclimation period, mice (n = 48) were randomized to diets containing ∼19% of total energy from walnuts, flaxseed oil, or corn oil (control) and were subsequently studied for 25 d.Results: Tumor growth rate was significantly slower in walnut-fed and flaxseed-fed mice compared with corn oil-fed animals (P < 0.05) by 27% and 43%, respectively. Accordingly, final tumor weight was reduced by 33% and 44%, respectively (P < 0.05 versus control); the differences between walnut and flaxseed diets did not reach significance. We found no differences among groups in metabolic and hormonal profile, serum antioxidant capacity, or inflammation (P > 0.05). However, walnuts and flaxseed oil significantly reduced serum expression levels of angiogenesis factors, including vascular endothelial growth factor (by 30% and 80%, respectively), and approximately doubled total necrotic areas despite smaller tumor sizes (P < 0.05 versus control). Dietary walnuts significantly decreased angiogenesis (CD34 staining; P = 0.017 versus control), whereas this effect did not reach significance in the flaxseed oil group (P = 0.454 versus control).Conclusion: We conclude that walnuts in the diet inhibit colorectal cancer growth by suppressing angiogenesis. Further studies are needed to confirm our findings in humans and explore underlying mechanisms.
KW - Angiogenesis Inhibitors/therapeutic use
KW - Animals
KW - Antigens, CD34/metabolism
KW - Antineoplastic Agents, Phytogenic/therapeutic use
KW - Colorectal Neoplasms/blood
KW - Dietary Supplements
KW - Female
KW - HT29 Cells
KW - Humans
KW - Juglans
KW - Linseed Oil/therapeutic use
KW - Mice
KW - Mice, Nude
KW - Necrosis
KW - Neovascularization, Pathologic/prevention & control
KW - Nuts
KW - Random Allocation
KW - Tumor Burden
KW - Vascular Endothelial Growth Factors/blood
KW - Xenograft Model Antitumor Assays
U2 - 10.1016/j.nut.2011.03.004
DO - 10.1016/j.nut.2011.03.004
M3 - Journal article
C2 - 21795022
VL - 28
SP - 67
EP - 75
JO - Nutrition
JF - Nutrition
SN - 0899-9007
IS - 1
ER -
ID: 290037367