TY - JOUR

T1 - Insulin sensitivity is not associated with palmitoleate availability in obese humans

AU - Fabbrini, Elisa

AU - Magkos, Faidon

AU - Su, Xiong

AU - Abumrad, Nada A

AU - Nejedly, Nicole

AU - Coughlin, Carrie C

AU - Okunade, Adewole L

AU - Patterson, Bruce W

AU - Klein, Samuel

N1 - (Ekstern)

PY - 2011

Y1 - 2011

N2 - We evaluated whether insulin resistance in obese people is associated with decreased plasma palmitoleate availability. Palmitoleate content (percentage and absolute concentrations) in FFA and VLDL was measured in obese subjects who were either insulin resistant (IR) or insulin sensitive (IS), based on assessment of multiorgan (skeletal muscle, liver, and adipose tissue) insulin sensitivity by using the hyperinsulinemic-euglycemic clamp procedure in conjunction with infusion of stable isotopically labeled tracers. Plasma palmitoleate concentration and the relative contribution of palmitoleate to total plasma FFA concentration in the IS group (0.018 ± 0.002 mmol/l and 4.4% ± 0.2%, respectively) were not significantly different than values in the IR group (0.023 ± 0.003 mmol/l and 4.4% ± 0.4%, respectively). Plasma VLDL-triglyceride palmitoleate concentration and the proportion of VLDL fatty acids as palmitoleate in the IS group (0.09 ± 0.02 mmol/l and 5.7 ± 0.3%, respectively) were also not significantly different than those in the IR group (0.16 ± 0.04 mmol/l and 5.0% ± 0.4%, respectively). These data demonstrate that decreased palmitoleate in plasma and in VLDL is not associated with insulin resistance in skeletal muscle, liver, or adipose tissue in obese people.

AB - We evaluated whether insulin resistance in obese people is associated with decreased plasma palmitoleate availability. Palmitoleate content (percentage and absolute concentrations) in FFA and VLDL was measured in obese subjects who were either insulin resistant (IR) or insulin sensitive (IS), based on assessment of multiorgan (skeletal muscle, liver, and adipose tissue) insulin sensitivity by using the hyperinsulinemic-euglycemic clamp procedure in conjunction with infusion of stable isotopically labeled tracers. Plasma palmitoleate concentration and the relative contribution of palmitoleate to total plasma FFA concentration in the IS group (0.018 ± 0.002 mmol/l and 4.4% ± 0.2%, respectively) were not significantly different than values in the IR group (0.023 ± 0.003 mmol/l and 4.4% ± 0.4%, respectively). Plasma VLDL-triglyceride palmitoleate concentration and the proportion of VLDL fatty acids as palmitoleate in the IS group (0.09 ± 0.02 mmol/l and 5.7 ± 0.3%, respectively) were also not significantly different than those in the IR group (0.16 ± 0.04 mmol/l and 5.0% ± 0.4%, respectively). These data demonstrate that decreased palmitoleate in plasma and in VLDL is not associated with insulin resistance in skeletal muscle, liver, or adipose tissue in obese people.

KW - Adipose Tissue/metabolism

KW - Adult

KW - Fatty Acids, Monounsaturated/blood

KW - Fatty Acids, Nonesterified/blood

KW - Female

KW - Glucose Clamp Technique

KW - Humans

KW - Insulin Resistance/physiology

KW - Lipoproteins, VLDL/blood

KW - Liver/metabolism

KW - Male

KW - Muscle, Skeletal/metabolism

KW - Obesity/blood

KW - Stearoyl-CoA Desaturase/genetics

U2 - 10.1194/jlr.M013599

DO - 10.1194/jlr.M013599

M3 - Journal article

C2 - 21266364

VL - 52

SP - 808

EP - 812

JO - Journal of Lipid Research

JF - Journal of Lipid Research

SN - 0022-2275

IS - 4

ER -