Spatial-proteomics reveal in-vivo phospho-signaling dynamics at subcellular resolution
Publikation: Working paper › Preprint › Forskning
Dynamic change in subcellular localization of signaling proteins is a general concept that eukaryotic cells evolved for eliciting a coordinated response to stimuli. Mass spectrometry (MS)-based proteomics in combination with subcellular fractionation can provide comprehensive maps of spatio-temporal regulation of cells, but involves laborious workflows that does not cover the phospho-proteome level. Here we present a high-throughput workflow based on sequential cell fractionation to profile the global proteome and phospho-proteome dynamics across six distinct subcellular fractions. We benchmarked the workflow by studying spatio-temporal EGFR phospho-signaling dynamics in-vitro in HeLa cells and in-vivo in mouse tissues. Finally, we investigated the spatio-temporal stress signaling, revealing cellular relocation of ribosomal proteins in response to hypertonicity and muscle contraction. Proteomics data generated in this study can be explored through https://SpatialProteoDynamics.github.io.
Originalsprog | Engelsk |
---|---|
Udgiver | bioRxiv |
Sider | 1-34 |
Antal sider | 34 |
DOI | |
Status | Udgivet - 2 feb. 2021 |
Bibliografisk note
(Preprint)
Links
- https://www.biorxiv.org/content/10.1101/2021.02.02.425898v1.full.pdf
Indsendt manuskript
ID: 328735289