Abundance of ClC-1 chloride channel in human skeletal muscle: Fibre type specific differences and effect of training

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Cl- channel protein 1 (ClC-1) may be important for excitability and contractility in skeletal muscle, but ClC-1 abundance has not been examined in human muscle. The aim of the present study was to examine ClC-1 abundance in human skeletal muscle, including fibre type specific differences and the effect of exercise training. A commercially available antibody was tested with positive and negative control tissue, and it recognised specifically ClC-1 in the range from 100-150 kDa. Abundance of ClC-1 was 38% higher (P < 0.01) in fast twitch Type IIa muscle fibres than in slow twitch Type I. Muscle ClC-1 abundance did not change with four weeks of training consisting of 30 min cycling at 85% of maximal heart rate (HRmax) and 3×30-s all out sprints or during a 7-week training period with 10-12×30 s uphill cycling and 4-5×~4 min cycling at 90-95% of HRmax. ClC-1 abundance correlated negatively (P < 0.01) with maximal oxygen consumption (r = -0.552) and incremental exercise performance (r = -0.546). In addition, trained cyclists had lower (P < 0.01) ClC-1 abundance than lesser trained individuals. The present observations indicate that a low abundance of muscle ClC-1 may be beneficial for exercise performance, but the role of abundance and regulation of ClC-1 in skeletal muscle of humans with respect to exercise performance and trainability need to be elucidated.

TidsskriftJournal of Applied Physiology
Udgave nummer2
Sider (fra-til)470-478
Antal sider9
StatusUdgivet - 2018

Bibliografisk note

CURIS 2018 NEXS 299

ID: 196202784