Abundance of ClC-1 chloride channel in human skeletal muscle: Fibre type specific differences and effect of training

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Standard

Abundance of ClC-1 chloride channel in human skeletal muscle : Fibre type specific differences and effect of training. / Thomassen, Martin; Hostrup, Morten; Murphy, Robyn M; Cromer, Brett A; Skovgaard, Casper; Gunnarsson, Thomas Petursson; Christensen, Peter Møller; Bangsbo, Jens.

I: Journal of Applied Physiology, Bind 125, Nr. 2, 2018, s. 470-478.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Thomassen, M, Hostrup, M, Murphy, RM, Cromer, BA, Skovgaard, C, Gunnarsson, TP, Christensen, PM & Bangsbo, J 2018, 'Abundance of ClC-1 chloride channel in human skeletal muscle: Fibre type specific differences and effect of training', Journal of Applied Physiology, bind 125, nr. 2, s. 470-478. https://doi.org/10.1152/japplphysiol.01042.2017

APA

Thomassen, M., Hostrup, M., Murphy, R. M., Cromer, B. A., Skovgaard, C., Gunnarsson, T. P., Christensen, P. M., & Bangsbo, J. (2018). Abundance of ClC-1 chloride channel in human skeletal muscle: Fibre type specific differences and effect of training. Journal of Applied Physiology, 125(2), 470-478. https://doi.org/10.1152/japplphysiol.01042.2017

Vancouver

Thomassen M, Hostrup M, Murphy RM, Cromer BA, Skovgaard C, Gunnarsson TP o.a. Abundance of ClC-1 chloride channel in human skeletal muscle: Fibre type specific differences and effect of training. Journal of Applied Physiology. 2018;125(2):470-478. https://doi.org/10.1152/japplphysiol.01042.2017

Author

Thomassen, Martin ; Hostrup, Morten ; Murphy, Robyn M ; Cromer, Brett A ; Skovgaard, Casper ; Gunnarsson, Thomas Petursson ; Christensen, Peter Møller ; Bangsbo, Jens. / Abundance of ClC-1 chloride channel in human skeletal muscle : Fibre type specific differences and effect of training. I: Journal of Applied Physiology. 2018 ; Bind 125, Nr. 2. s. 470-478.

Bibtex

@article{1e6565d016394868bc8e60f31962d189,
title = "Abundance of ClC-1 chloride channel in human skeletal muscle: Fibre type specific differences and effect of training",
abstract = "Cl- channel protein 1 (ClC-1) may be important for excitability and contractility in skeletal muscle, but ClC-1 abundance has not been examined in human muscle. The aim of the present study was to examine ClC-1 abundance in human skeletal muscle, including fibre type specific differences and the effect of exercise training. A commercially available antibody was tested with positive and negative control tissue, and it recognised specifically ClC-1 in the range from 100-150 kDa. Abundance of ClC-1 was 38% higher (P < 0.01) in fast twitch Type IIa muscle fibres than in slow twitch Type I. Muscle ClC-1 abundance did not change with four weeks of training consisting of 30 min cycling at 85% of maximal heart rate (HRmax) and 3×30-s all out sprints or during a 7-week training period with 10-12×30 s uphill cycling and 4-5×~4 min cycling at 90-95% of HRmax. ClC-1 abundance correlated negatively (P < 0.01) with maximal oxygen consumption (r = -0.552) and incremental exercise performance (r = -0.546). In addition, trained cyclists had lower (P < 0.01) ClC-1 abundance than lesser trained individuals. The present observations indicate that a low abundance of muscle ClC-1 may be beneficial for exercise performance, but the role of abundance and regulation of ClC-1 in skeletal muscle of humans with respect to exercise performance and trainability need to be elucidated.",
keywords = "Faculty of Science, Single fibre, Excitability, Performance, Antibody specificity, ADR, Arrested development of righting response, Proteine expression, Single fiber",
author = "Martin Thomassen and Morten Hostrup and Murphy, {Robyn M} and Cromer, {Brett A} and Casper Skovgaard and Gunnarsson, {Thomas Petursson} and Christensen, {Peter M{\o}ller} and Jens Bangsbo",
note = "CURIS 2018 NEXS 299",
year = "2018",
doi = "10.1152/japplphysiol.01042.2017",
language = "English",
volume = "125",
pages = "470--478",
journal = "Journal of Applied Physiology",
issn = "8750-7587",
publisher = "American Physiological Society",
number = "2",

}

RIS

TY - JOUR

T1 - Abundance of ClC-1 chloride channel in human skeletal muscle

T2 - Fibre type specific differences and effect of training

AU - Thomassen, Martin

AU - Hostrup, Morten

AU - Murphy, Robyn M

AU - Cromer, Brett A

AU - Skovgaard, Casper

AU - Gunnarsson, Thomas Petursson

AU - Christensen, Peter Møller

AU - Bangsbo, Jens

N1 - CURIS 2018 NEXS 299

PY - 2018

Y1 - 2018

N2 - Cl- channel protein 1 (ClC-1) may be important for excitability and contractility in skeletal muscle, but ClC-1 abundance has not been examined in human muscle. The aim of the present study was to examine ClC-1 abundance in human skeletal muscle, including fibre type specific differences and the effect of exercise training. A commercially available antibody was tested with positive and negative control tissue, and it recognised specifically ClC-1 in the range from 100-150 kDa. Abundance of ClC-1 was 38% higher (P < 0.01) in fast twitch Type IIa muscle fibres than in slow twitch Type I. Muscle ClC-1 abundance did not change with four weeks of training consisting of 30 min cycling at 85% of maximal heart rate (HRmax) and 3×30-s all out sprints or during a 7-week training period with 10-12×30 s uphill cycling and 4-5×~4 min cycling at 90-95% of HRmax. ClC-1 abundance correlated negatively (P < 0.01) with maximal oxygen consumption (r = -0.552) and incremental exercise performance (r = -0.546). In addition, trained cyclists had lower (P < 0.01) ClC-1 abundance than lesser trained individuals. The present observations indicate that a low abundance of muscle ClC-1 may be beneficial for exercise performance, but the role of abundance and regulation of ClC-1 in skeletal muscle of humans with respect to exercise performance and trainability need to be elucidated.

AB - Cl- channel protein 1 (ClC-1) may be important for excitability and contractility in skeletal muscle, but ClC-1 abundance has not been examined in human muscle. The aim of the present study was to examine ClC-1 abundance in human skeletal muscle, including fibre type specific differences and the effect of exercise training. A commercially available antibody was tested with positive and negative control tissue, and it recognised specifically ClC-1 in the range from 100-150 kDa. Abundance of ClC-1 was 38% higher (P < 0.01) in fast twitch Type IIa muscle fibres than in slow twitch Type I. Muscle ClC-1 abundance did not change with four weeks of training consisting of 30 min cycling at 85% of maximal heart rate (HRmax) and 3×30-s all out sprints or during a 7-week training period with 10-12×30 s uphill cycling and 4-5×~4 min cycling at 90-95% of HRmax. ClC-1 abundance correlated negatively (P < 0.01) with maximal oxygen consumption (r = -0.552) and incremental exercise performance (r = -0.546). In addition, trained cyclists had lower (P < 0.01) ClC-1 abundance than lesser trained individuals. The present observations indicate that a low abundance of muscle ClC-1 may be beneficial for exercise performance, but the role of abundance and regulation of ClC-1 in skeletal muscle of humans with respect to exercise performance and trainability need to be elucidated.

KW - Faculty of Science

KW - Single fibre

KW - Excitability

KW - Performance

KW - Antibody specificity

KW - ADR

KW - Arrested development of righting response

KW - Proteine expression

KW - Single fiber

U2 - 10.1152/japplphysiol.01042.2017

DO - 10.1152/japplphysiol.01042.2017

M3 - Journal article

C2 - 29722626

VL - 125

SP - 470

EP - 478

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

SN - 8750-7587

IS - 2

ER -

ID: 196202784