Validation of a novel index to assess insulin resistance of adipose tissue lipolytic activity in obese subjects
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Validation of a novel index to assess insulin resistance of adipose tissue lipolytic activity in obese subjects. / Fabbrini, Elisa; Magkos, Faidon; Conte, Caterina; Mittendorfer, Bettina; Patterson, Bruce W; Okunade, Adewole L; Klein, Samuel.
I: Journal of Lipid Research, Bind 53, Nr. 2, 2012, s. 321-324.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Validation of a novel index to assess insulin resistance of adipose tissue lipolytic activity in obese subjects
AU - Fabbrini, Elisa
AU - Magkos, Faidon
AU - Conte, Caterina
AU - Mittendorfer, Bettina
AU - Patterson, Bruce W
AU - Okunade, Adewole L
AU - Klein, Samuel
N1 - (Ekstern)
PY - 2012
Y1 - 2012
N2 - Insulin resistance in adipose tissue increases the release of free fatty acids into the circulation, which likely contributes to impaired insulin action in liver and skeletal muscle associated with obesity. However, reliable assessment of adipose tissue insulin resistance requires performing a hyperinsulinemic-euglycemic clamp procedure in conjunction with a fatty acid tracer infusion to determine insulin-mediated suppression of lipolytic rate. We developed a simpler method for evaluating adipose tissue insulin resistance in vivo, determined as the product of palmitate rate of appearance into the bloodstream and plasma insulin concentration during basal conditions. We validated our Adipose Tissue Insulin Resistance Index (ATIRI) by comparison with an assessment of adipose tissue insulin resistance determined by using the hyperinsulinemic-euglycemic clamp procedure in conjunction with a palmitate tracer infusion in 47 obese nondiabetic subjects (body mass index: 40.1 ± 9.3 kg/m(2)). We found the ATIRI correlated closely with adipose tissue insulin resistance assessed during the clamp procedure (r =-0.854, P < 0.001). These results demonstrate that the ATIRI provides a reliable index of adipose tissue insulin resistance in obese subjects.
AB - Insulin resistance in adipose tissue increases the release of free fatty acids into the circulation, which likely contributes to impaired insulin action in liver and skeletal muscle associated with obesity. However, reliable assessment of adipose tissue insulin resistance requires performing a hyperinsulinemic-euglycemic clamp procedure in conjunction with a fatty acid tracer infusion to determine insulin-mediated suppression of lipolytic rate. We developed a simpler method for evaluating adipose tissue insulin resistance in vivo, determined as the product of palmitate rate of appearance into the bloodstream and plasma insulin concentration during basal conditions. We validated our Adipose Tissue Insulin Resistance Index (ATIRI) by comparison with an assessment of adipose tissue insulin resistance determined by using the hyperinsulinemic-euglycemic clamp procedure in conjunction with a palmitate tracer infusion in 47 obese nondiabetic subjects (body mass index: 40.1 ± 9.3 kg/m(2)). We found the ATIRI correlated closely with adipose tissue insulin resistance assessed during the clamp procedure (r =-0.854, P < 0.001). These results demonstrate that the ATIRI provides a reliable index of adipose tissue insulin resistance in obese subjects.
KW - Adipose Tissue/metabolism
KW - Adult
KW - Body Mass Index
KW - Female
KW - Glucose Clamp Technique/methods
KW - Humans
KW - Insulin Resistance/physiology
KW - Male
KW - Middle Aged
KW - Obesity/metabolism
KW - Palmitates/blood
U2 - 10.1194/jlr.D020321
DO - 10.1194/jlr.D020321
M3 - Journal article
C2 - 22147838
VL - 53
SP - 321
EP - 324
JO - Journal of Lipid Research
JF - Journal of Lipid Research
SN - 0022-2275
IS - 2
ER -
ID: 290036001