Training state and fasting-induced PDH regulation in human skeletal muscle
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Training state and fasting-induced PDH regulation in human skeletal muscle. / Gudiksen, Anders; Bertholdt, Lærke; Stankiewicz, Tomasz; Villesen, Ida; Bangsbo, Jens; Plomgaard, Peter; Pilegaard, Henriette.
I: Pflügers Archiv - European Journal of Physiology, Bind 470, Nr. 11, 2018, s. 1633-1645.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Training state and fasting-induced PDH regulation in human skeletal muscle
AU - Gudiksen, Anders
AU - Bertholdt, Lærke
AU - Stankiewicz, Tomasz
AU - Villesen, Ida
AU - Bangsbo, Jens
AU - Plomgaard, Peter
AU - Pilegaard, Henriette
N1 - CURIS 2018 NEXS 238
PY - 2018
Y1 - 2018
N2 - The aim of the present study was to examine the influence of training state on fasting-induced skeletal muscle pyruvate dehydrogenase (PDH) regulation, including PDH phosphorylation. Trained and untrained subjects, matched for skeletal muscle CS activity and OXPHOS protein, fasted for 36 h after receiving a standardized meal. Respiratory exchange ratio (RER) was measured and blood as well as vastus lateralis muscle biopsies were obtained 2, 12, 24, and 36 h after the meal. RER decreased with fasting only in untrained individuals, while PDHa activity decreased from 12 h after the meal in untrained, but only tended to decrease at 36 h in trained. PDH-E1α, PDP1 protein, PDH phosphorylation, and PDH acetylation in skeletal muscle was higher in trained than untrained subjects, but did not change with fasting, while PDK4 protein was higher at 36 h than at 2 h after the meal in both groups. In conclusion, the present results suggest that endurance exercise training modifies the fasting-induced regulation of PDHa activity in skeletal muscle and the substrate switch towards fat oxidation. PDH phosphorylation could not explain the fasting-induced regulation of PDHa activity suggesting other post translational modifications.
AB - The aim of the present study was to examine the influence of training state on fasting-induced skeletal muscle pyruvate dehydrogenase (PDH) regulation, including PDH phosphorylation. Trained and untrained subjects, matched for skeletal muscle CS activity and OXPHOS protein, fasted for 36 h after receiving a standardized meal. Respiratory exchange ratio (RER) was measured and blood as well as vastus lateralis muscle biopsies were obtained 2, 12, 24, and 36 h after the meal. RER decreased with fasting only in untrained individuals, while PDHa activity decreased from 12 h after the meal in untrained, but only tended to decrease at 36 h in trained. PDH-E1α, PDP1 protein, PDH phosphorylation, and PDH acetylation in skeletal muscle was higher in trained than untrained subjects, but did not change with fasting, while PDK4 protein was higher at 36 h than at 2 h after the meal in both groups. In conclusion, the present results suggest that endurance exercise training modifies the fasting-induced regulation of PDHa activity in skeletal muscle and the substrate switch towards fat oxidation. PDH phosphorylation could not explain the fasting-induced regulation of PDHa activity suggesting other post translational modifications.
KW - Acetylation
KW - Exercise training
KW - Fasting
KW - Phosphorylation
KW - Pyruvate dehydrogenase
KW - Skeletal muscle
U2 - 10.1007/s00424-018-2164-6
DO - 10.1007/s00424-018-2164-6
M3 - Journal article
C2 - 29946822
AN - SCOPUS:85049106987
VL - 470
SP - 1633
EP - 1645
JO - Pflügers Archiv - European Journal of Physiology
JF - Pflügers Archiv - European Journal of Physiology
SN - 0031-6768
IS - 11
ER -
ID: 200286271