Supplement with whey protein hydrolysate in contrast to carbohydrate supports mitochondrial adaptations in trained runners

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Standard

Supplement with whey protein hydrolysate in contrast to carbohydrate supports mitochondrial adaptations in trained runners. / Hansen, Mette; Oxfeldt, Mikkel; Larsen, Anne E; Thomsen, Lise S; Rokkedal-Lausch, Torben; Christensen, Britt; Rittig, Nikolaj; de Paoli, Frank Vincenzo; Bangsbo, Jens; Ørtenblad, Niels; Madsen, Klavs.

I: Journal of the International Society of Sports Nutrition, Bind 17, 46, 2020.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Hansen, M, Oxfeldt, M, Larsen, AE, Thomsen, LS, Rokkedal-Lausch, T, Christensen, B, Rittig, N, de Paoli, FV, Bangsbo, J, Ørtenblad, N & Madsen, K 2020, 'Supplement with whey protein hydrolysate in contrast to carbohydrate supports mitochondrial adaptations in trained runners', Journal of the International Society of Sports Nutrition, bind 17, 46. https://doi.org/10.1186/s12970-020-00376-3

APA

Hansen, M., Oxfeldt, M., Larsen, A. E., Thomsen, L. S., Rokkedal-Lausch, T., Christensen, B., Rittig, N., de Paoli, F. V., Bangsbo, J., Ørtenblad, N., & Madsen, K. (2020). Supplement with whey protein hydrolysate in contrast to carbohydrate supports mitochondrial adaptations in trained runners. Journal of the International Society of Sports Nutrition, 17, [46]. https://doi.org/10.1186/s12970-020-00376-3

Vancouver

Hansen M, Oxfeldt M, Larsen AE, Thomsen LS, Rokkedal-Lausch T, Christensen B o.a. Supplement with whey protein hydrolysate in contrast to carbohydrate supports mitochondrial adaptations in trained runners. Journal of the International Society of Sports Nutrition. 2020;17. 46. https://doi.org/10.1186/s12970-020-00376-3

Author

Hansen, Mette ; Oxfeldt, Mikkel ; Larsen, Anne E ; Thomsen, Lise S ; Rokkedal-Lausch, Torben ; Christensen, Britt ; Rittig, Nikolaj ; de Paoli, Frank Vincenzo ; Bangsbo, Jens ; Ørtenblad, Niels ; Madsen, Klavs. / Supplement with whey protein hydrolysate in contrast to carbohydrate supports mitochondrial adaptations in trained runners. I: Journal of the International Society of Sports Nutrition. 2020 ; Bind 17.

Bibtex

@article{3c5798b71c8f422ca50bcc71961cef4b,
title = "Supplement with whey protein hydrolysate in contrast to carbohydrate supports mitochondrial adaptations in trained runners",
abstract = "Background: Protein supplementation has been suggested to augment endurance training adaptations by increasing mixed muscle and myofibrillar protein synthesis and lean body mass. However, a potential beneficial effect on mitochondrial adaptations is yet to be clarified. The aim of the present study was to investigate the effect of consuming whey protein hydrolysate before and whey protein hydrolysate plus carbohydrate (PRO-CHO) after each exercise session during a six-week training period compared to similarly timed intake of isocaloric CHO supplements on biomarkers of mitochondrial biogenesis, VO2max and performance in trained runners.Methods: Twenty-four trained runners (VO2max 60.7 ± 3.7 ml O2 kg- 1 min1) completed a six-week block randomized controlled intervention period, consisting of progressive running training. Subjects were randomly assigned to either PRO-CHO or CHO and matched in pairs for gender, age, VO2max, training and performance status. The PRO-CHO group ingested a protein beverage (0.3 g kg- 1) before and protein-carbohydrate beverage (0.3 g protein kg- 1 and 1 g carbohydrate kg- 1) after each exercise session. The CHO group ingested an energy matched carbohydrate beverage. Resting muscle biopsies obtained pre and post intervention were analyzed for mitochondrial specific enzyme activity and mitochondrial protein content. Subjects completed a 6 K time trial (6 K TT) and a VO2max test pre, midway (only 6 K TT) and post intervention.Results: Following six weeks of endurance training Cytochrome C (Cyt C) protein content was significantly higher in the PRO-CHO group compared to the CHO group (p < 0.05), with several other mitochondrial proteins (Succinate dehydrogenase (SDHA), Cytochrome C oxidase (COX-IV), Voltage-dependent anion channel (VDAC), Heat shock protein 60 (HSP60), and Prohibitin (PHB1)) following a similar, but non-significant pattern (p = 0.07-0.14). β-hydroxyacyl-CoA dehydrogenase (HAD) activity was significantly lower after training in the CHO group (p < 0.01), but not in the PRO-CHO group (p = 0.24). VO2max and 6 K TT was significantly improved after training with no significant difference between groups.Conclusion: Intake of whey PRO hydrolysate before and whey PRO hydrolysate plus CHO after each exercise session during a six-week endurance training period may augment training effects on specific mitochondrial proteins compared to intake of iso-caloric CHO but does not alter VO2max or 6 K TT performance.Trial registration: clinicaltrials.gov , NCT03561337 . Registered 6 June 2018 - Retrospectively registered.",
keywords = "Faculty of Science, Sports nutrition, Protein hydrolysate, Performance, Enzyme activity, Skeletal muscle, Mitochondria, Endurance sport",
author = "Mette Hansen and Mikkel Oxfeldt and Larsen, {Anne E} and Thomsen, {Lise S} and Torben Rokkedal-Lausch and Britt Christensen and Nikolaj Rittig and {de Paoli}, {Frank Vincenzo} and Jens Bangsbo and Niels {\O}rtenblad and Klavs Madsen",
note = "CURIS 2020 NEXS 289",
year = "2020",
doi = "10.1186/s12970-020-00376-3",
language = "English",
volume = "17",
journal = "Journal of the International Society of Sports Nutrition",
issn = "1550-2783",
publisher = "BioMed Central (BMC)",

}

RIS

TY - JOUR

T1 - Supplement with whey protein hydrolysate in contrast to carbohydrate supports mitochondrial adaptations in trained runners

AU - Hansen, Mette

AU - Oxfeldt, Mikkel

AU - Larsen, Anne E

AU - Thomsen, Lise S

AU - Rokkedal-Lausch, Torben

AU - Christensen, Britt

AU - Rittig, Nikolaj

AU - de Paoli, Frank Vincenzo

AU - Bangsbo, Jens

AU - Ørtenblad, Niels

AU - Madsen, Klavs

N1 - CURIS 2020 NEXS 289

PY - 2020

Y1 - 2020

N2 - Background: Protein supplementation has been suggested to augment endurance training adaptations by increasing mixed muscle and myofibrillar protein synthesis and lean body mass. However, a potential beneficial effect on mitochondrial adaptations is yet to be clarified. The aim of the present study was to investigate the effect of consuming whey protein hydrolysate before and whey protein hydrolysate plus carbohydrate (PRO-CHO) after each exercise session during a six-week training period compared to similarly timed intake of isocaloric CHO supplements on biomarkers of mitochondrial biogenesis, VO2max and performance in trained runners.Methods: Twenty-four trained runners (VO2max 60.7 ± 3.7 ml O2 kg- 1 min1) completed a six-week block randomized controlled intervention period, consisting of progressive running training. Subjects were randomly assigned to either PRO-CHO or CHO and matched in pairs for gender, age, VO2max, training and performance status. The PRO-CHO group ingested a protein beverage (0.3 g kg- 1) before and protein-carbohydrate beverage (0.3 g protein kg- 1 and 1 g carbohydrate kg- 1) after each exercise session. The CHO group ingested an energy matched carbohydrate beverage. Resting muscle biopsies obtained pre and post intervention were analyzed for mitochondrial specific enzyme activity and mitochondrial protein content. Subjects completed a 6 K time trial (6 K TT) and a VO2max test pre, midway (only 6 K TT) and post intervention.Results: Following six weeks of endurance training Cytochrome C (Cyt C) protein content was significantly higher in the PRO-CHO group compared to the CHO group (p < 0.05), with several other mitochondrial proteins (Succinate dehydrogenase (SDHA), Cytochrome C oxidase (COX-IV), Voltage-dependent anion channel (VDAC), Heat shock protein 60 (HSP60), and Prohibitin (PHB1)) following a similar, but non-significant pattern (p = 0.07-0.14). β-hydroxyacyl-CoA dehydrogenase (HAD) activity was significantly lower after training in the CHO group (p < 0.01), but not in the PRO-CHO group (p = 0.24). VO2max and 6 K TT was significantly improved after training with no significant difference between groups.Conclusion: Intake of whey PRO hydrolysate before and whey PRO hydrolysate plus CHO after each exercise session during a six-week endurance training period may augment training effects on specific mitochondrial proteins compared to intake of iso-caloric CHO but does not alter VO2max or 6 K TT performance.Trial registration: clinicaltrials.gov , NCT03561337 . Registered 6 June 2018 - Retrospectively registered.

AB - Background: Protein supplementation has been suggested to augment endurance training adaptations by increasing mixed muscle and myofibrillar protein synthesis and lean body mass. However, a potential beneficial effect on mitochondrial adaptations is yet to be clarified. The aim of the present study was to investigate the effect of consuming whey protein hydrolysate before and whey protein hydrolysate plus carbohydrate (PRO-CHO) after each exercise session during a six-week training period compared to similarly timed intake of isocaloric CHO supplements on biomarkers of mitochondrial biogenesis, VO2max and performance in trained runners.Methods: Twenty-four trained runners (VO2max 60.7 ± 3.7 ml O2 kg- 1 min1) completed a six-week block randomized controlled intervention period, consisting of progressive running training. Subjects were randomly assigned to either PRO-CHO or CHO and matched in pairs for gender, age, VO2max, training and performance status. The PRO-CHO group ingested a protein beverage (0.3 g kg- 1) before and protein-carbohydrate beverage (0.3 g protein kg- 1 and 1 g carbohydrate kg- 1) after each exercise session. The CHO group ingested an energy matched carbohydrate beverage. Resting muscle biopsies obtained pre and post intervention were analyzed for mitochondrial specific enzyme activity and mitochondrial protein content. Subjects completed a 6 K time trial (6 K TT) and a VO2max test pre, midway (only 6 K TT) and post intervention.Results: Following six weeks of endurance training Cytochrome C (Cyt C) protein content was significantly higher in the PRO-CHO group compared to the CHO group (p < 0.05), with several other mitochondrial proteins (Succinate dehydrogenase (SDHA), Cytochrome C oxidase (COX-IV), Voltage-dependent anion channel (VDAC), Heat shock protein 60 (HSP60), and Prohibitin (PHB1)) following a similar, but non-significant pattern (p = 0.07-0.14). β-hydroxyacyl-CoA dehydrogenase (HAD) activity was significantly lower after training in the CHO group (p < 0.01), but not in the PRO-CHO group (p = 0.24). VO2max and 6 K TT was significantly improved after training with no significant difference between groups.Conclusion: Intake of whey PRO hydrolysate before and whey PRO hydrolysate plus CHO after each exercise session during a six-week endurance training period may augment training effects on specific mitochondrial proteins compared to intake of iso-caloric CHO but does not alter VO2max or 6 K TT performance.Trial registration: clinicaltrials.gov , NCT03561337 . Registered 6 June 2018 - Retrospectively registered.

KW - Faculty of Science

KW - Sports nutrition

KW - Protein hydrolysate

KW - Performance

KW - Enzyme activity

KW - Skeletal muscle

KW - Mitochondria

KW - Endurance sport

U2 - 10.1186/s12970-020-00376-3

DO - 10.1186/s12970-020-00376-3

M3 - Journal article

C2 - 32894140

VL - 17

JO - Journal of the International Society of Sports Nutrition

JF - Journal of the International Society of Sports Nutrition

SN - 1550-2783

M1 - 46

ER -

ID: 248192816