TY - JOUR

T1 - Short chain fatty acids and bile acids in human faeces are associated with the intestinal cholesterol conversion status

AU - Matysik, Silke

AU - Krautbauer, Sabrina

AU - Liebisch, Gerhard

AU - Schött, Hans-Frieder

AU - Kjølbæk, Louise

AU - Astrup, Arne

AU - Blachier, Francois

AU - Beaumont, Martin

AU - Nieuwdorp, Max

AU - Hartstra, Annick

AU - Rampelli, Simone

AU - Pagotto, Uberto

AU - Iozzo, Patricia

N1 - This article is protected by copyright. All rights reserved.

PY - 2021

Y1 - 2021

N2 - Background and purpose: The analysis of human faecal metabolites can provide an insight into metabolic interactions between gut microbiota and host organism. The creation of metabolic profiles in faeces has received little attention until now and reference values, especially in the context of dietary and therapeutic interventions, are missing. Exposure to xenobiotics significantly affects the physiology of the microbiome and microbiota manipulation as well as short chain fatty acids administration have been proposed as treatment targets for several diseases. The aim of the present study is to give concomitant concentration ranges of faecal sterol species, bile acids and short chain fatty acids based on a large cohort.Experimental approach: Sterol species, bile acids and short chain fatty acids in human faeces from 165 study participants were quantified by LC-MS/MS. For standardization, we refer all values to dry weight of faeces. Based on the individual intestinal sterol conversion we classified participants into low and high converters according to their coprostanol/cholesterol ratio.Key results: Low converters excrete more straight chain fatty acids and bile acids than high converters. 5th , 95th percentile and median of bile acids and short chain fatty acids were calculated for both groups.Conclusion and implications: We give concentration ranges for 16 faecal metabolites that can serve as reference values. Patient stratification into high or low sterol converter groups is associated with significant differences in faecal metabolites with biological activities. Such stratification should then allow assessing faecal metabolites better before therapeutic interventions.

AB - Background and purpose: The analysis of human faecal metabolites can provide an insight into metabolic interactions between gut microbiota and host organism. The creation of metabolic profiles in faeces has received little attention until now and reference values, especially in the context of dietary and therapeutic interventions, are missing. Exposure to xenobiotics significantly affects the physiology of the microbiome and microbiota manipulation as well as short chain fatty acids administration have been proposed as treatment targets for several diseases. The aim of the present study is to give concomitant concentration ranges of faecal sterol species, bile acids and short chain fatty acids based on a large cohort.Experimental approach: Sterol species, bile acids and short chain fatty acids in human faeces from 165 study participants were quantified by LC-MS/MS. For standardization, we refer all values to dry weight of faeces. Based on the individual intestinal sterol conversion we classified participants into low and high converters according to their coprostanol/cholesterol ratio.Key results: Low converters excrete more straight chain fatty acids and bile acids than high converters. 5th , 95th percentile and median of bile acids and short chain fatty acids were calculated for both groups.Conclusion and implications: We give concentration ranges for 16 faecal metabolites that can serve as reference values. Patient stratification into high or low sterol converter groups is associated with significant differences in faecal metabolites with biological activities. Such stratification should then allow assessing faecal metabolites better before therapeutic interventions.

KW - Faculty of Science

KW - Bile acids

KW - Faecal short chain fatty acids

KW - Sterols

U2 - 10.1111/bph.15440

DO - 10.1111/bph.15440

M3 - Journal article

C2 - 33751575

VL - 178

SP - 3342

EP - 3353

JO - British Journal of Pharmacology

JF - British Journal of Pharmacology

SN - 0007-1188

IS - 16

ER -