TY - JOUR

T1 - Optimized, fast through-put UHPLC-DAD based method for carotenoid quantification in spinach, serum, chylomicrons and faeces

AU - Eriksen, Jane Nygaard

AU - Madsen, Pia Lisbeth

AU - Dragsted, Lars Ove

AU - Arrigoni, Eva

N1 - CURIS 2017 NEXS 030

PY - 2017

Y1 - 2017

N2 - An improved UHPLC-DAD based method was developed and validated for quantification of major carotenoids present in spinach, serum, chylomicrons and faeces. Separation was achieved with gradient elution within 12.5 min for 6 dietary carotenoids and the internal standard, echinenone. The proposed method provides, for all standard components, resolution >1.1, linearity covering the target range (R>0.99), LOQ <0.035 mg/L, intra-day and inter-day RSD <2% and 10%, respectively. Suitability of the method was tested on biological matrices. Method precision (RSD%) for carotenoid quantification in serum, chylomicrons and faeces was below 10% for intra- and inter-day analysis, except for lycopene. Method accuracy was consistent with mean recoveries ranging from 78.8-96.9 and 57.2-96.9% for all carotenoids, except for lycopene, in serum and faeces, respectively. Additionally, an inter-laboratory validation study on spinach at two institutions showed no significant differences in lutein or β-carotene content, when evaluated at 4 occasions.

AB - An improved UHPLC-DAD based method was developed and validated for quantification of major carotenoids present in spinach, serum, chylomicrons and faeces. Separation was achieved with gradient elution within 12.5 min for 6 dietary carotenoids and the internal standard, echinenone. The proposed method provides, for all standard components, resolution >1.1, linearity covering the target range (R>0.99), LOQ <0.035 mg/L, intra-day and inter-day RSD <2% and 10%, respectively. Suitability of the method was tested on biological matrices. Method precision (RSD%) for carotenoid quantification in serum, chylomicrons and faeces was below 10% for intra- and inter-day analysis, except for lycopene. Method accuracy was consistent with mean recoveries ranging from 78.8-96.9 and 57.2-96.9% for all carotenoids, except for lycopene, in serum and faeces, respectively. Additionally, an inter-laboratory validation study on spinach at two institutions showed no significant differences in lutein or β-carotene content, when evaluated at 4 occasions.

KW - Faculty of Science

KW - UHPLC-DAD

KW - Lutein

KW - Beta-carotene

KW - Green leafy vegetables

KW - Human samples

U2 - 10.1021/acs.jafc.6b04925

DO - 10.1021/acs.jafc.6b04925

M3 - Journal article

C2 - 28004571

VL - 65

SP - 973

EP - 980

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 4

ER -