Increased FXYD1 and PGC-1α mRNA after blood flow-restricted running is related to fibre type-specific AMPK signalling and oxidative stress in human muscle

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Increased FXYD1 and PGC-1α mRNA after blood flow-restricted running is related to fibre type-specific AMPK signalling and oxidative stress in human muscle. / Christiansen, Danny; Murphy, Robyn M; Bangsbo, Jens; Stathis, Christos G; Bishop, David J.

I: Acta Physiologica (Print), Bind 223, Nr. 2, e13045, 2018.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Christiansen, D, Murphy, RM, Bangsbo, J, Stathis, CG & Bishop, DJ 2018, 'Increased FXYD1 and PGC-1α mRNA after blood flow-restricted running is related to fibre type-specific AMPK signalling and oxidative stress in human muscle', Acta Physiologica (Print), bind 223, nr. 2, e13045. https://doi.org/10.1111/apha.13045

APA

Christiansen, D., Murphy, R. M., Bangsbo, J., Stathis, C. G., & Bishop, D. J. (2018). Increased FXYD1 and PGC-1α mRNA after blood flow-restricted running is related to fibre type-specific AMPK signalling and oxidative stress in human muscle. Acta Physiologica (Print), 223(2), [e13045]. https://doi.org/10.1111/apha.13045

Vancouver

Christiansen D, Murphy RM, Bangsbo J, Stathis CG, Bishop DJ. Increased FXYD1 and PGC-1α mRNA after blood flow-restricted running is related to fibre type-specific AMPK signalling and oxidative stress in human muscle. Acta Physiologica (Print). 2018;223(2). e13045. https://doi.org/10.1111/apha.13045

Author

Christiansen, Danny ; Murphy, Robyn M ; Bangsbo, Jens ; Stathis, Christos G ; Bishop, David J. / Increased FXYD1 and PGC-1α mRNA after blood flow-restricted running is related to fibre type-specific AMPK signalling and oxidative stress in human muscle. I: Acta Physiologica (Print). 2018 ; Bind 223, Nr. 2.

Bibtex

@article{65bb2758db6e4948a5a07d89d98e5223,
title = "Increased FXYD1 and PGC-1α mRNA after blood flow-restricted running is related to fibre type-specific AMPK signalling and oxidative stress in human muscle",
abstract = "AIM: This study explored the effects of blood flow restriction (BFR) on mRNA responses of PGC-1α (total, 1α1, and 1α4) and Na+ ,K+ -ATPase isoforms (NKA; α1-3 , β1-3 , and FXYD1) to an interval running session, and determined if these effects were related to increased oxidative stress, hypoxia, and fibre type-specific AMPK and CaMKII signalling, in human skeletal muscle.METHODS: In a randomised, crossover fashion, eight healthy men (26 ± 5 y and 57.4 ± 6.3 mL∙kg-1 ∙min-1 ) completed three exercise sessions: without (CON) or with blood flow restriction (BFR), or in systemic hypoxia (HYP, ~3250 m). A muscle sample was collected before (Pre) and after exercise (+0h, +3h) to quantify mRNA, indicators of oxidative stress (HSP27 protein in type I and II fibres, and catalase and HSP70 mRNA), metabolites, and α-AMPK Thr172 /α-AMPK, ACC Ser221 /ACC, CaMKII Thr287 /CaMKII, and PLBSer16 /PLB ratios in type I and II fibres.RESULTS: Muscle hypoxia (assessed by near-infrared spectroscopy) was matched between BFR and HYP, which was higher than CON (~90{\%} vs. ~70{\%}; p<0.05). The mRNA levels of FXYD1 and PGC-1α isoforms (1α1 and 1α4) increased in BFR only (p<0.05) and were associated with increases in indicators of oxidative stress and type-I fibre ACC Ser221 /ACC ratio, but dissociated from muscle hypoxia, lactate, and CaMKII signalling.CONCLUSION: Blood flow restriction augmented exercise-induced increases in muscle FXYD1 and PGC-1α mRNA in men. This effect was related to increased oxidative stress and fibre type-dependent AMPK signalling, but unrelated to the severity of muscle hypoxia, lactate accumulation, and modulation of fibre type-specific CaMKII signalling. This article is protected by copyright. All rights reserved.",
keywords = "AMPK, Blood flow restriction, Na+-K+-ATPase, Oxidative stress, PGC-1α, Reactive oxygen species",
author = "Danny Christiansen and Murphy, {Robyn M} and Jens Bangsbo and Stathis, {Christos G} and Bishop, {David J}",
note = "CURIS 2018 NEXS 082",
year = "2018",
doi = "10.1111/apha.13045",
language = "English",
volume = "223",
journal = "Acta Physiologica (Print)",
issn = "1748-1708",
publisher = "Wiley-Blackwell",
number = "2",

}

RIS

TY - JOUR

T1 - Increased FXYD1 and PGC-1α mRNA after blood flow-restricted running is related to fibre type-specific AMPK signalling and oxidative stress in human muscle

AU - Christiansen, Danny

AU - Murphy, Robyn M

AU - Bangsbo, Jens

AU - Stathis, Christos G

AU - Bishop, David J

N1 - CURIS 2018 NEXS 082

PY - 2018

Y1 - 2018

N2 - AIM: This study explored the effects of blood flow restriction (BFR) on mRNA responses of PGC-1α (total, 1α1, and 1α4) and Na+ ,K+ -ATPase isoforms (NKA; α1-3 , β1-3 , and FXYD1) to an interval running session, and determined if these effects were related to increased oxidative stress, hypoxia, and fibre type-specific AMPK and CaMKII signalling, in human skeletal muscle.METHODS: In a randomised, crossover fashion, eight healthy men (26 ± 5 y and 57.4 ± 6.3 mL∙kg-1 ∙min-1 ) completed three exercise sessions: without (CON) or with blood flow restriction (BFR), or in systemic hypoxia (HYP, ~3250 m). A muscle sample was collected before (Pre) and after exercise (+0h, +3h) to quantify mRNA, indicators of oxidative stress (HSP27 protein in type I and II fibres, and catalase and HSP70 mRNA), metabolites, and α-AMPK Thr172 /α-AMPK, ACC Ser221 /ACC, CaMKII Thr287 /CaMKII, and PLBSer16 /PLB ratios in type I and II fibres.RESULTS: Muscle hypoxia (assessed by near-infrared spectroscopy) was matched between BFR and HYP, which was higher than CON (~90% vs. ~70%; p<0.05). The mRNA levels of FXYD1 and PGC-1α isoforms (1α1 and 1α4) increased in BFR only (p<0.05) and were associated with increases in indicators of oxidative stress and type-I fibre ACC Ser221 /ACC ratio, but dissociated from muscle hypoxia, lactate, and CaMKII signalling.CONCLUSION: Blood flow restriction augmented exercise-induced increases in muscle FXYD1 and PGC-1α mRNA in men. This effect was related to increased oxidative stress and fibre type-dependent AMPK signalling, but unrelated to the severity of muscle hypoxia, lactate accumulation, and modulation of fibre type-specific CaMKII signalling. This article is protected by copyright. All rights reserved.

AB - AIM: This study explored the effects of blood flow restriction (BFR) on mRNA responses of PGC-1α (total, 1α1, and 1α4) and Na+ ,K+ -ATPase isoforms (NKA; α1-3 , β1-3 , and FXYD1) to an interval running session, and determined if these effects were related to increased oxidative stress, hypoxia, and fibre type-specific AMPK and CaMKII signalling, in human skeletal muscle.METHODS: In a randomised, crossover fashion, eight healthy men (26 ± 5 y and 57.4 ± 6.3 mL∙kg-1 ∙min-1 ) completed three exercise sessions: without (CON) or with blood flow restriction (BFR), or in systemic hypoxia (HYP, ~3250 m). A muscle sample was collected before (Pre) and after exercise (+0h, +3h) to quantify mRNA, indicators of oxidative stress (HSP27 protein in type I and II fibres, and catalase and HSP70 mRNA), metabolites, and α-AMPK Thr172 /α-AMPK, ACC Ser221 /ACC, CaMKII Thr287 /CaMKII, and PLBSer16 /PLB ratios in type I and II fibres.RESULTS: Muscle hypoxia (assessed by near-infrared spectroscopy) was matched between BFR and HYP, which was higher than CON (~90% vs. ~70%; p<0.05). The mRNA levels of FXYD1 and PGC-1α isoforms (1α1 and 1α4) increased in BFR only (p<0.05) and were associated with increases in indicators of oxidative stress and type-I fibre ACC Ser221 /ACC ratio, but dissociated from muscle hypoxia, lactate, and CaMKII signalling.CONCLUSION: Blood flow restriction augmented exercise-induced increases in muscle FXYD1 and PGC-1α mRNA in men. This effect was related to increased oxidative stress and fibre type-dependent AMPK signalling, but unrelated to the severity of muscle hypoxia, lactate accumulation, and modulation of fibre type-specific CaMKII signalling. This article is protected by copyright. All rights reserved.

KW - AMPK

KW - Blood flow restriction

KW - Na+-K+-ATPase

KW - Oxidative stress

KW - PGC-1α

KW - Reactive oxygen species

U2 - 10.1111/apha.13045

DO - 10.1111/apha.13045

M3 - Journal article

C2 - 29383885

VL - 223

JO - Acta Physiologica (Print)

JF - Acta Physiologica (Print)

SN - 1748-1708

IS - 2

M1 - e13045

ER -

ID: 189152871