Deoxyribonucleoside kinases in two aquatic bacteria with high specificity for thymidine and deoxyadenosine

Publikation: Bidrag til tidsskrift › Letter › Forskning › fagfællebedømt

Standard

Deoxyribonucleoside kinases in two aquatic bacteria with high specificity for thymidine and deoxyadenosine. / Tinta, Tinkara; Christiansen, Louise Slot; Konrad, Anke; Liberles, David A; Turk, Valentina; Munch-Petersen, Birgitte; Piškur, Jure; Clausen, Anders R.

I: F E M S Microbiology Letters, Bind 331, Nr. 2, 2012, s. 120-127.

Publikation: Bidrag til tidsskrift › Letter › Forskning › fagfællebedømt

Harvard

Tinta, T, Christiansen, LS, Konrad, A, Liberles, DA, Turk, V, Munch-Petersen, B, Piškur, J & Clausen, AR 2012, 'Deoxyribonucleoside kinases in two aquatic bacteria with high specificity for thymidine and deoxyadenosine', F E M S Microbiology Letters, bind 331, nr. 2, s. 120-127. https://doi.org/10.1111/j.1574-6968.2012.02565.x

APA

Tinta, T., Christiansen, L. S., Konrad, A., Liberles, D. A., Turk, V., Munch-Petersen, B., Piškur, J., & Clausen, A. R. (2012). Deoxyribonucleoside kinases in two aquatic bacteria with high specificity for thymidine and deoxyadenosine. F E M S Microbiology Letters, 331(2), 120-127. https://doi.org/10.1111/j.1574-6968.2012.02565.x

Vancouver

Tinta T, Christiansen LS, Konrad A, Liberles DA, Turk V, Munch-Petersen B o.a. Deoxyribonucleoside kinases in two aquatic bacteria with high specificity for thymidine and deoxyadenosine. F E M S Microbiology Letters. 2012;331(2):120-127. https://doi.org/10.1111/j.1574-6968.2012.02565.x

Author

Tinta, Tinkara ; Christiansen, Louise Slot ; Konrad, Anke ; Liberles, David A ; Turk, Valentina ; Munch-Petersen, Birgitte ; Piškur, Jure ; Clausen, Anders R. / Deoxyribonucleoside kinases in two aquatic bacteria with high specificity for thymidine and deoxyadenosine. I: F E M S Microbiology Letters. 2012 ; Bind 331, Nr. 2. s. 120-127.

Bibtex

@article{8710cbaed3ce4c37ae05a83078c16b0c,
title = "Deoxyribonucleoside kinases in two aquatic bacteria with high specificity for thymidine and deoxyadenosine",
abstract = "Deoxyribonucleoside kinases (dNKs) are essential in the mammalian cell but their 'importance' in bacteria, especially aquatic ones, is less clear. We studied two aquatic bacteria, Gram-negative Flavobacterium psychrophilum JIP02/86 and Polaribacter sp. MED152, for their ability to salvage deoxyribonucleosides (dNs). Both had a Gram-positive-type thymidine kinase (TK1), which could phosphorylate thymidine, and one non-TK1 dNK, which could efficiently phosphorylate deoxyadenosine and slightly also deoxycytosine. Surprisingly, the four tested dNKs could not phosphorylate deoxyguanosine, and apparently, these two bacteria are missing this activity. When tens of available aquatic bacteria genomes were examined for the presence of dNKs, a majority had at least a TK1-like gene, but several lacked any dNKs. Apparently, among aquatic bacteria, the role of the dN salvage varies.",
keywords = "H-thymidine incorporation, Aquatic bacteria, Deoxyribonucleoside kinase, Nucleoside salvage",
author = "Tinkara Tinta and Christiansen, {Louise Slot} and Anke Konrad and Liberles, {David A} and Valentina Turk and Birgitte Munch-Petersen and Jure Pi{\v s}kur and Clausen, {Anders R}",
note = "(Ekstern)",
year = "2012",
doi = "10.1111/j.1574-6968.2012.02565.x",
language = "English",
volume = "331",
pages = "120--127",
journal = "F E M S Microbiology Letters",
issn = "0378-1097",
publisher = "Oxford University Press",
number = "2",

}

RIS

TY - JOUR

T1 - Deoxyribonucleoside kinases in two aquatic bacteria with high specificity for thymidine and deoxyadenosine

AU - Tinta, Tinkara

AU - Christiansen, Louise Slot

AU - Konrad, Anke

AU - Liberles, David A

AU - Turk, Valentina

AU - Munch-Petersen, Birgitte

AU - Piškur, Jure

AU - Clausen, Anders R

N1 - (Ekstern)

PY - 2012

Y1 - 2012

N2 - Deoxyribonucleoside kinases (dNKs) are essential in the mammalian cell but their 'importance' in bacteria, especially aquatic ones, is less clear. We studied two aquatic bacteria, Gram-negative Flavobacterium psychrophilum JIP02/86 and Polaribacter sp. MED152, for their ability to salvage deoxyribonucleosides (dNs). Both had a Gram-positive-type thymidine kinase (TK1), which could phosphorylate thymidine, and one non-TK1 dNK, which could efficiently phosphorylate deoxyadenosine and slightly also deoxycytosine. Surprisingly, the four tested dNKs could not phosphorylate deoxyguanosine, and apparently, these two bacteria are missing this activity. When tens of available aquatic bacteria genomes were examined for the presence of dNKs, a majority had at least a TK1-like gene, but several lacked any dNKs. Apparently, among aquatic bacteria, the role of the dN salvage varies.

AB - Deoxyribonucleoside kinases (dNKs) are essential in the mammalian cell but their 'importance' in bacteria, especially aquatic ones, is less clear. We studied two aquatic bacteria, Gram-negative Flavobacterium psychrophilum JIP02/86 and Polaribacter sp. MED152, for their ability to salvage deoxyribonucleosides (dNs). Both had a Gram-positive-type thymidine kinase (TK1), which could phosphorylate thymidine, and one non-TK1 dNK, which could efficiently phosphorylate deoxyadenosine and slightly also deoxycytosine. Surprisingly, the four tested dNKs could not phosphorylate deoxyguanosine, and apparently, these two bacteria are missing this activity. When tens of available aquatic bacteria genomes were examined for the presence of dNKs, a majority had at least a TK1-like gene, but several lacked any dNKs. Apparently, among aquatic bacteria, the role of the dN salvage varies.

KW - H-thymidine incorporation

KW - Aquatic bacteria

KW - Deoxyribonucleoside kinase

KW - Nucleoside salvage

UR - http://www.scopus.com/inward/record.url?scp=84861233766&partnerID=8YFLogxK

U2 - 10.1111/j.1574-6968.2012.02565.x

DO - 10.1111/j.1574-6968.2012.02565.x

M3 - Letter

C2 - 22462611

AN - SCOPUS:84861233766

VL - 331

SP - 120

EP - 127

JO - F E M S Microbiology Letters

JF - F E M S Microbiology Letters

SN - 0378-1097

IS - 2

ER -

ID: 255883926