Cycling with blood flow restriction improves performance and muscle K+ regulation and alters the effect of antioxidant infusion in humans

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Cycling with blood flow restriction improves performance and muscle K+ regulation and alters the effect of antioxidant infusion in humans. / Christiansen, Danny; Eibye, Kasper Hvid; Rasmussen, Villads; Voldby, Hans Møller; Thomassen, Martin; Nyberg, Michael Permin; Gunnarsson, Thomas Gunnar Petursson; Skovgaard, Casper; Lindskrog, Mads Søgaard; Bishop, David J; Hostrup, Morten; Bangsbo, Jens.

I: Journal of Physiology, Bind 597, Nr. 9, 2019, s. 2421-2444.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Christiansen, D, Eibye, KH, Rasmussen, V, Voldby, HM, Thomassen, M, Nyberg, MP, Gunnarsson, TGP, Skovgaard, C, Lindskrog, MS, Bishop, DJ, Hostrup, M & Bangsbo, J 2019, 'Cycling with blood flow restriction improves performance and muscle K+ regulation and alters the effect of antioxidant infusion in humans', Journal of Physiology, bind 597, nr. 9, s. 2421-2444. https://doi.org/10.1113/JP277657

APA

Christiansen, D., Eibye, K. H., Rasmussen, V., Voldby, H. M., Thomassen, M., Nyberg, M. P., ... Bangsbo, J. (2019). Cycling with blood flow restriction improves performance and muscle K+ regulation and alters the effect of antioxidant infusion in humans. Journal of Physiology, 597(9), 2421-2444. https://doi.org/10.1113/JP277657

Vancouver

Christiansen D, Eibye KH, Rasmussen V, Voldby HM, Thomassen M, Nyberg MP o.a. Cycling with blood flow restriction improves performance and muscle K+ regulation and alters the effect of antioxidant infusion in humans. Journal of Physiology. 2019;597(9):2421-2444. https://doi.org/10.1113/JP277657

Author

Christiansen, Danny ; Eibye, Kasper Hvid ; Rasmussen, Villads ; Voldby, Hans Møller ; Thomassen, Martin ; Nyberg, Michael Permin ; Gunnarsson, Thomas Gunnar Petursson ; Skovgaard, Casper ; Lindskrog, Mads Søgaard ; Bishop, David J ; Hostrup, Morten ; Bangsbo, Jens. / Cycling with blood flow restriction improves performance and muscle K+ regulation and alters the effect of antioxidant infusion in humans. I: Journal of Physiology. 2019 ; Bind 597, Nr. 9. s. 2421-2444.

Bibtex

@article{54fde935e91a476f8da46243ba63de79,
title = "Cycling with blood flow restriction improves performance and muscle K+ regulation and alters the effect of antioxidant infusion in humans",
abstract = "Key points: Training with blood flow restriction (BFR) is a well-recognised strategy to promote muscle hypertrophy and strength. However, its potential to enhance muscle function during sustained, intense exercise remains largely unexplored. Here, we report that interval training with BFR augments improvements in performance and reduces net K+ release from contracting muscles during high-intensity exercise in active men. A better K+ regulation after BFR-training is associated with an elevated blood flow to exercising muscles and altered muscle antioxidant function, as indicated by a higher reduced to oxidised glutathione (GSH:GSSG) ratio, compared to control, and an increased thigh net K+ release during intense exercise with concomitant antioxidant infusion. Training with BFR also invoked fibre-type specific adaptations in abundance of Na+,K+-ATPase isoforms (α1 , β1 , phospholemman/FXYD1). Thus, BFR-training enhances performance and K+ regulation during intense exercise, which may be due to adaptations in antioxidant function, blood flow, and Na+,K+-ATPase-isoform abundance at the fibre-type level.Abstract: We examined if blood flow restriction (BFR) augments training-induced improvements in K+ regulation and performance during intense exercise in men, and if these adaptations are associated with an altered muscle antioxidant function, blood flow, and/or with fibre type-dependent changes in Na+,K+-ATPase-isoform abundance. Ten recreationally-active men (25 ± 4 y, 49.7 ± 5.3 mL kg-1 min-1) performed 6 weeks of interval cycling, where one leg trained without (control; CON-leg) and the other with BFR (BFR-leg, pressure: ∼180 mmHg). Before and after training, femoral arterial and venous K+ concentrations and artery blood flow were measured during single-leg knee-extensor exercise at 25{\%} (Ex1) and 90{\%} of thigh incremental peak power (Ex2) with intravenous infusion of N-acetylcysteine (NAC) or placebo (saline), and a resting muscle biopsy was collected. After training, performance increased more in BFR-leg (23{\%}) than in CON-leg (12{\%}, p<0.05), whereas K+ release during Ex2 was attenuated only from BFR-leg (p < 0.05). Muscle GSH:GSSG ratio at rest and blood flow during exercise were higher in BFR-leg than in CON-leg after training (p < 0.05). After training, NAC increased resting muscle GSH concentration and thigh net K+ release during Ex2 only in BFR-leg (p < 0.05), whilst the abundance of Na+,K+-ATPase-isoform α1 in type-II (51{\%}), β1 in type-I (33{\%}), and FXYD1 in type-I (108{\%}) and type-II (60{\%}) fibres was higher in BFR-leg than in CON-leg (p < 0.05). Thus, training with BFR elicited greater improvements in performance and reduced thigh K+ release during intense exercise, which were associated with adaptations in muscle antioxidant function, blood flow, and Na+,K+-ATPase-isoform abundance at the fibre-type level.",
keywords = "Faculty of Science, Blood flow restriction training, Human muscle, Antioxidant, Reactive oxygen species, N-acetylcysteine, Fibre type, Single type, Na+,K+-ATPase, Ion transport",
author = "Danny Christiansen and Eibye, {Kasper Hvid} and Villads Rasmussen and Voldby, {Hans M{\o}ller} and Martin Thomassen and Nyberg, {Michael Permin} and Gunnarsson, {Thomas Gunnar Petursson} and Casper Skovgaard and Lindskrog, {Mads S{\o}gaard} and Bishop, {David J} and Morten Hostrup and Jens Bangsbo",
note = "CURIS 2019 NEXS 110 This article is protected by copyright. All rights reserved.",
year = "2019",
doi = "10.1113/JP277657",
language = "English",
volume = "597",
pages = "2421--2444",
journal = "The Journal of Physiology",
issn = "0022-3751",
publisher = "Wiley-Blackwell",
number = "9",

}

RIS

TY - JOUR

T1 - Cycling with blood flow restriction improves performance and muscle K+ regulation and alters the effect of antioxidant infusion in humans

AU - Christiansen, Danny

AU - Eibye, Kasper Hvid

AU - Rasmussen, Villads

AU - Voldby, Hans Møller

AU - Thomassen, Martin

AU - Nyberg, Michael Permin

AU - Gunnarsson, Thomas Gunnar Petursson

AU - Skovgaard, Casper

AU - Lindskrog, Mads Søgaard

AU - Bishop, David J

AU - Hostrup, Morten

AU - Bangsbo, Jens

N1 - CURIS 2019 NEXS 110 This article is protected by copyright. All rights reserved.

PY - 2019

Y1 - 2019

N2 - Key points: Training with blood flow restriction (BFR) is a well-recognised strategy to promote muscle hypertrophy and strength. However, its potential to enhance muscle function during sustained, intense exercise remains largely unexplored. Here, we report that interval training with BFR augments improvements in performance and reduces net K+ release from contracting muscles during high-intensity exercise in active men. A better K+ regulation after BFR-training is associated with an elevated blood flow to exercising muscles and altered muscle antioxidant function, as indicated by a higher reduced to oxidised glutathione (GSH:GSSG) ratio, compared to control, and an increased thigh net K+ release during intense exercise with concomitant antioxidant infusion. Training with BFR also invoked fibre-type specific adaptations in abundance of Na+,K+-ATPase isoforms (α1 , β1 , phospholemman/FXYD1). Thus, BFR-training enhances performance and K+ regulation during intense exercise, which may be due to adaptations in antioxidant function, blood flow, and Na+,K+-ATPase-isoform abundance at the fibre-type level.Abstract: We examined if blood flow restriction (BFR) augments training-induced improvements in K+ regulation and performance during intense exercise in men, and if these adaptations are associated with an altered muscle antioxidant function, blood flow, and/or with fibre type-dependent changes in Na+,K+-ATPase-isoform abundance. Ten recreationally-active men (25 ± 4 y, 49.7 ± 5.3 mL kg-1 min-1) performed 6 weeks of interval cycling, where one leg trained without (control; CON-leg) and the other with BFR (BFR-leg, pressure: ∼180 mmHg). Before and after training, femoral arterial and venous K+ concentrations and artery blood flow were measured during single-leg knee-extensor exercise at 25% (Ex1) and 90% of thigh incremental peak power (Ex2) with intravenous infusion of N-acetylcysteine (NAC) or placebo (saline), and a resting muscle biopsy was collected. After training, performance increased more in BFR-leg (23%) than in CON-leg (12%, p<0.05), whereas K+ release during Ex2 was attenuated only from BFR-leg (p < 0.05). Muscle GSH:GSSG ratio at rest and blood flow during exercise were higher in BFR-leg than in CON-leg after training (p < 0.05). After training, NAC increased resting muscle GSH concentration and thigh net K+ release during Ex2 only in BFR-leg (p < 0.05), whilst the abundance of Na+,K+-ATPase-isoform α1 in type-II (51%), β1 in type-I (33%), and FXYD1 in type-I (108%) and type-II (60%) fibres was higher in BFR-leg than in CON-leg (p < 0.05). Thus, training with BFR elicited greater improvements in performance and reduced thigh K+ release during intense exercise, which were associated with adaptations in muscle antioxidant function, blood flow, and Na+,K+-ATPase-isoform abundance at the fibre-type level.

AB - Key points: Training with blood flow restriction (BFR) is a well-recognised strategy to promote muscle hypertrophy and strength. However, its potential to enhance muscle function during sustained, intense exercise remains largely unexplored. Here, we report that interval training with BFR augments improvements in performance and reduces net K+ release from contracting muscles during high-intensity exercise in active men. A better K+ regulation after BFR-training is associated with an elevated blood flow to exercising muscles and altered muscle antioxidant function, as indicated by a higher reduced to oxidised glutathione (GSH:GSSG) ratio, compared to control, and an increased thigh net K+ release during intense exercise with concomitant antioxidant infusion. Training with BFR also invoked fibre-type specific adaptations in abundance of Na+,K+-ATPase isoforms (α1 , β1 , phospholemman/FXYD1). Thus, BFR-training enhances performance and K+ regulation during intense exercise, which may be due to adaptations in antioxidant function, blood flow, and Na+,K+-ATPase-isoform abundance at the fibre-type level.Abstract: We examined if blood flow restriction (BFR) augments training-induced improvements in K+ regulation and performance during intense exercise in men, and if these adaptations are associated with an altered muscle antioxidant function, blood flow, and/or with fibre type-dependent changes in Na+,K+-ATPase-isoform abundance. Ten recreationally-active men (25 ± 4 y, 49.7 ± 5.3 mL kg-1 min-1) performed 6 weeks of interval cycling, where one leg trained without (control; CON-leg) and the other with BFR (BFR-leg, pressure: ∼180 mmHg). Before and after training, femoral arterial and venous K+ concentrations and artery blood flow were measured during single-leg knee-extensor exercise at 25% (Ex1) and 90% of thigh incremental peak power (Ex2) with intravenous infusion of N-acetylcysteine (NAC) or placebo (saline), and a resting muscle biopsy was collected. After training, performance increased more in BFR-leg (23%) than in CON-leg (12%, p<0.05), whereas K+ release during Ex2 was attenuated only from BFR-leg (p < 0.05). Muscle GSH:GSSG ratio at rest and blood flow during exercise were higher in BFR-leg than in CON-leg after training (p < 0.05). After training, NAC increased resting muscle GSH concentration and thigh net K+ release during Ex2 only in BFR-leg (p < 0.05), whilst the abundance of Na+,K+-ATPase-isoform α1 in type-II (51%), β1 in type-I (33%), and FXYD1 in type-I (108%) and type-II (60%) fibres was higher in BFR-leg than in CON-leg (p < 0.05). Thus, training with BFR elicited greater improvements in performance and reduced thigh K+ release during intense exercise, which were associated with adaptations in muscle antioxidant function, blood flow, and Na+,K+-ATPase-isoform abundance at the fibre-type level.

KW - Faculty of Science

KW - Blood flow restriction training

KW - Human muscle

KW - Antioxidant

KW - Reactive oxygen species

KW - N-acetylcysteine

KW - Fibre type

KW - Single type

KW - Na+,K+-ATPase

KW - Ion transport

U2 - 10.1113/JP277657

DO - 10.1113/JP277657

M3 - Journal article

C2 - 30843602

VL - 597

SP - 2421

EP - 2444

JO - The Journal of Physiology

JF - The Journal of Physiology

SN - 0022-3751

IS - 9

ER -

ID: 214685326