TY - JOUR

T1 - Column-switching high-performance liquid chromatographic assay for determination of apigenin and acacetin in human urine with ultraviolet absorbance detection

AU - Nielsen, Salka E

AU - Dragsted, Lars Ove

N1 - (Ekstern)

PY - 1998

Y1 - 1998

N2 - A high-performance liquid chromatographic (HPLC) method is described for the determination of apigenin and the 4'-methylated derivative acacetin in human urine using column-switching and ultraviolet (UV) absorbance detection. Urine samples were enzymatically hydrolysed and solid-phase extracted prior to injection onto the HPLC system. Prior to elution of apigenin and the internal standard, 5,7,8-trihydroxyflavone, from the first column used for sample clean-up, the six-port valve was switched to the second column for analysis with UV detection. Detection of apigenin was precise and reproducible, with a limit of quantification of 10 ngml-1 urine. Detection and quantification of acacetin was linear down to 70 ngml-1 urine. The method has been successfully applied to determine the level of apigenin in 100 human urine samples from an intervention study with parsley. Copyright (C) 1998 Elsevier Science B.V.

AB - A high-performance liquid chromatographic (HPLC) method is described for the determination of apigenin and the 4'-methylated derivative acacetin in human urine using column-switching and ultraviolet (UV) absorbance detection. Urine samples were enzymatically hydrolysed and solid-phase extracted prior to injection onto the HPLC system. Prior to elution of apigenin and the internal standard, 5,7,8-trihydroxyflavone, from the first column used for sample clean-up, the six-port valve was switched to the second column for analysis with UV detection. Detection of apigenin was precise and reproducible, with a limit of quantification of 10 ngml-1 urine. Detection and quantification of acacetin was linear down to 70 ngml-1 urine. The method has been successfully applied to determine the level of apigenin in 100 human urine samples from an intervention study with parsley. Copyright (C) 1998 Elsevier Science B.V.

KW - Acacetin

KW - Apigenin

UR - http://www.scopus.com/inward/record.url?scp=0032424649&partnerID=8YFLogxK

U2 - 10.1016/S0378-4347(98)00187-X

DO - 10.1016/S0378-4347(98)00187-X

M3 - Journal article

C2 - 9746253

AN - SCOPUS:0032424649

VL - 713

SP - 379

EP - 386

JO - Journal of Chromatography - Biomedical Applications

JF - Journal of Chromatography - Biomedical Applications

SN - 0378-4347

IS - 2

ER -