A triple quadrupole and a hybrid quadrupole orbitrap mass spectrometer in comparison for polyphenol quantitation
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A triple quadrupole and a hybrid quadrupole orbitrap mass spectrometer in comparison for polyphenol quantitation. / Cavaliere, Chiara; Antonelli, Michela; Capriotti, Anna Laura; La Barbera, Giorgia; Montone, Carmela Maria; Piovesana, Susy; Laganà, Aldo.
I: Journal of Agricultural and Food Chemistry, Bind 67, Nr. 17, 01.05.2019, s. 4885-4896.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - A triple quadrupole and a hybrid quadrupole orbitrap mass spectrometer in comparison for polyphenol quantitation
AU - Cavaliere, Chiara
AU - Antonelli, Michela
AU - Capriotti, Anna Laura
AU - La Barbera, Giorgia
AU - Montone, Carmela Maria
AU - Piovesana, Susy
AU - Laganà, Aldo
N1 - (Ekstern)
PY - 2019/5/1
Y1 - 2019/5/1
N2 - Liquid chromatography coupled to low-resolution mass spectrometry (LRMS) has historically been a popular approach for compound quantitation. Recently, high-resolution mass spectrometry (HRMS) technical developments led to the introduction of new approaches for quantitative analysis. Whereas the performances of HRMS have been largely assessed for qualitative purposes, there are still questions about its suitability for quantitative analysis. Several papers on LRMS and HRMS comparison have been published; however, none of them was applied to polyphenol quantitation. In this work, a comparison between HRMS, operated in data-dependent acquisition mode, and LRMS, operated in selected-reaction-monitoring mode, was performed for polyphenol quantitation in wine. The two techniques were evaluated in terms of sensitivity, linearity range, matrix effect, and precision, showing the better performances of HRMS. The suitability of HRMS for quantitation purposes as well as qualitative screening makes HRMS the new technique of choice for both targeted and untargeted analysis.
AB - Liquid chromatography coupled to low-resolution mass spectrometry (LRMS) has historically been a popular approach for compound quantitation. Recently, high-resolution mass spectrometry (HRMS) technical developments led to the introduction of new approaches for quantitative analysis. Whereas the performances of HRMS have been largely assessed for qualitative purposes, there are still questions about its suitability for quantitative analysis. Several papers on LRMS and HRMS comparison have been published; however, none of them was applied to polyphenol quantitation. In this work, a comparison between HRMS, operated in data-dependent acquisition mode, and LRMS, operated in selected-reaction-monitoring mode, was performed for polyphenol quantitation in wine. The two techniques were evaluated in terms of sensitivity, linearity range, matrix effect, and precision, showing the better performances of HRMS. The suitability of HRMS for quantitation purposes as well as qualitative screening makes HRMS the new technique of choice for both targeted and untargeted analysis.
KW - High-resolution mass spectrometry
KW - Low-resolution mass spectrometry
KW - Polyphenols
KW - Quantitation
KW - Wine
UR - http://www.scopus.com/inward/record.url?scp=85065076894&partnerID=8YFLogxK
U2 - 10.1021/acs.jafc.8b07163
DO - 10.1021/acs.jafc.8b07163
M3 - Journal article
C2 - 30977362
AN - SCOPUS:85065076894
VL - 67
SP - 4885
EP - 4896
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
SN - 0021-8561
IS - 17
ER -
ID: 231310240