Saliva as a source of new phosphopeptide biomarkers: Development of a comprehensive analytical method based on shotgun peptidomics
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Saliva as a source of new phosphopeptide biomarkers : Development of a comprehensive analytical method based on shotgun peptidomics. / La Barbera, Giorgia; Capriotti, Anna Laura; Cavaliere, Chiara; Ferraris, Francesca; Montone, Carmela Maria; Piovesana, Susy; Zenezini Chiozzi, Riccardo; Laganà, Aldo.
In: Talanta, Vol. 183, 2018, p. 245-249.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Saliva as a source of new phosphopeptide biomarkers
T2 - Development of a comprehensive analytical method based on shotgun peptidomics
AU - La Barbera, Giorgia
AU - Capriotti, Anna Laura
AU - Cavaliere, Chiara
AU - Ferraris, Francesca
AU - Montone, Carmela Maria
AU - Piovesana, Susy
AU - Zenezini Chiozzi, Riccardo
AU - Laganà, Aldo
N1 - (Ekstern)
PY - 2018
Y1 - 2018
N2 - The paper describes the development of an enrichment method for the analysis of the endogenous phosphopeptides in saliva. The method was based on magnetic solid phase extraction by a magnetic graphitized carbon black-TiO2 composite material and was developed considering different saliva pre-treatments, namely C18 solid phase extraction for purification, direct dilution in loading buffer or acetonitrile precipitation. The method was based on a shotgun proteomics workflow and the enriched peptide mixture was analysed by nanoHPLC and high resolution tandem mass spectrometry. Acetonitrile precipitation provided the best results, with up to 165 endogenous phosphopeptides identified in saliva samples from healthy individuals. The physico-chemical features of the identified endogenous phosphopeptides indicated that such peptides were large, hydrophilic and basic.
AB - The paper describes the development of an enrichment method for the analysis of the endogenous phosphopeptides in saliva. The method was based on magnetic solid phase extraction by a magnetic graphitized carbon black-TiO2 composite material and was developed considering different saliva pre-treatments, namely C18 solid phase extraction for purification, direct dilution in loading buffer or acetonitrile precipitation. The method was based on a shotgun proteomics workflow and the enriched peptide mixture was analysed by nanoHPLC and high resolution tandem mass spectrometry. Acetonitrile precipitation provided the best results, with up to 165 endogenous phosphopeptides identified in saliva samples from healthy individuals. The physico-chemical features of the identified endogenous phosphopeptides indicated that such peptides were large, hydrophilic and basic.
KW - Endogenous saliva phosphopeptides
KW - Magnetic solid phase extraction
KW - Metal oxide affinity chromatography
KW - NanoHPLC-M/MS
KW - Phosphopeptide enrichment
KW - Phosphoproteomics
UR - http://www.scopus.com/inward/record.url?scp=85042518590&partnerID=8YFLogxK
U2 - 10.1016/j.talanta.2018.02.085
DO - 10.1016/j.talanta.2018.02.085
M3 - Journal article
C2 - 29567172
AN - SCOPUS:85042518590
VL - 183
SP - 245
EP - 249
JO - Talanta
JF - Talanta
SN - 0039-9140
ER -
ID: 231312595