Resting metabolic rate and skeletal muscle SERCA and Na+/K+ ATPase activities are not affected by fish oil supplementation in healthy older adults
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Resting metabolic rate and skeletal muscle SERCA and Na+/K+ ATPase activities are not affected by fish oil supplementation in healthy older adults. / Jannas-Vela, Sebastian; Klingel, Shannon L; Cervone, Daniel T; Wickham, Kate Aiko; Heigenhauser, George J F; Mutch, David M; Holloway, Graham P; Spriet, Lawrence L.
In: Physiological Reports, Vol. 8, No. 9, e14408, 2020.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Resting metabolic rate and skeletal muscle SERCA and Na+/K+ ATPase activities are not affected by fish oil supplementation in healthy older adults
AU - Jannas-Vela, Sebastian
AU - Klingel, Shannon L
AU - Cervone, Daniel T
AU - Wickham, Kate Aiko
AU - Heigenhauser, George J F
AU - Mutch, David M
AU - Holloway, Graham P
AU - Spriet, Lawrence L
N1 - (Ekstern)
PY - 2020
Y1 - 2020
N2 - Omega-3 polyunsaturated fatty acids (PUFAs) have unique properties purported to influence several aspects of metabolism, including energy expenditure and protein function. Supplementing with n-3 PUFAs may increase whole-body resting metabolic rate (RMR), by enhancing Na+/K+ ATPase (NKA) activity and reducing the efficiency of sarcoplasmic reticulum (SR) Ca2+ ATPase (SERCA) activity by inducing a Ca2+ leak-pump cycle. The purpose of this study was to examine the effects of fish oil (FO) on RMR, substrate oxidation, and skeletal muscle SERCA and NKA pump function in healthy older individuals. Subjects (n = 16 females; n = 8 males; 65 ± 1 years) were randomly assigned into groups supplemented with either olive oil (OO) (5 g/day) or FO (5 g/day) containing 2 g/day eicosapentaenoic acid and 1 g/day docosahexaenoic acid for 12 weeks. Participants visited the laboratory for RMR and substrate oxidation measurements after an overnight fast at weeks 0 and 12. Skeletal muscle biopsies were taken during weeks 0 and 12 for analysis of NKA and SERCA function and protein content. There was a main effect of time with decrease in RMR (5%) and fat oxidation (18%) in both the supplementation groups. The kinetic parameters of SERCA and NKA maximal activity, as well as the expression of SR and NKA proteins, were not affected after OO and FO supplementation. In conclusion, these results suggest that FO supplementation is not effective in altering RMR, substrate oxidation, and skeletal muscle SERCA and NKA protein levels and activities, in healthy older men and women.
AB - Omega-3 polyunsaturated fatty acids (PUFAs) have unique properties purported to influence several aspects of metabolism, including energy expenditure and protein function. Supplementing with n-3 PUFAs may increase whole-body resting metabolic rate (RMR), by enhancing Na+/K+ ATPase (NKA) activity and reducing the efficiency of sarcoplasmic reticulum (SR) Ca2+ ATPase (SERCA) activity by inducing a Ca2+ leak-pump cycle. The purpose of this study was to examine the effects of fish oil (FO) on RMR, substrate oxidation, and skeletal muscle SERCA and NKA pump function in healthy older individuals. Subjects (n = 16 females; n = 8 males; 65 ± 1 years) were randomly assigned into groups supplemented with either olive oil (OO) (5 g/day) or FO (5 g/day) containing 2 g/day eicosapentaenoic acid and 1 g/day docosahexaenoic acid for 12 weeks. Participants visited the laboratory for RMR and substrate oxidation measurements after an overnight fast at weeks 0 and 12. Skeletal muscle biopsies were taken during weeks 0 and 12 for analysis of NKA and SERCA function and protein content. There was a main effect of time with decrease in RMR (5%) and fat oxidation (18%) in both the supplementation groups. The kinetic parameters of SERCA and NKA maximal activity, as well as the expression of SR and NKA proteins, were not affected after OO and FO supplementation. In conclusion, these results suggest that FO supplementation is not effective in altering RMR, substrate oxidation, and skeletal muscle SERCA and NKA protein levels and activities, in healthy older men and women.
KW - Na/K ATPase
KW - Omega-3
KW - Resting metabolic rate
KW - SERCA
KW - Skeletal muscle
U2 - 10.14814/phy2.14408
DO - 10.14814/phy2.14408
M3 - Journal article
C2 - 32342642
AN - SCOPUS:85084169720
VL - 8
JO - Physiological Reports
JF - Physiological Reports
SN - 2051-817X
IS - 9
M1 - e14408
ER -
ID: 254661398