Protein intake at twice the RDA in older men increases circulatory concentrations of the microbiome metabolite Trimethylamine-N-Oxide (TMAO)
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Protein intake at twice the RDA in older men increases circulatory concentrations of the microbiome metabolite Trimethylamine-N-Oxide (TMAO). / Mitchell, Sarah M; Milan, Amber M; Mitchell, Cameron J; Gillies, Nicola A; D'Souza, Randall F; Zeng, Nina; Ramzan, Farha; Sharma, Pankaja; Knowles, Scott O; Roy, Nicole C; Sjödin, Anders Mikael; Wagner, Karl-Heinz; Zeisel, Steven H; Cameron-Smith, David.
In: Nutrients, Vol. 11, No. 9, 2207, 2019.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Protein intake at twice the RDA in older men increases circulatory concentrations of the microbiome metabolite Trimethylamine-N-Oxide (TMAO)
AU - Mitchell, Sarah M
AU - Milan, Amber M
AU - Mitchell, Cameron J
AU - Gillies, Nicola A
AU - D'Souza, Randall F
AU - Zeng, Nina
AU - Ramzan, Farha
AU - Sharma, Pankaja
AU - Knowles, Scott O
AU - Roy, Nicole C
AU - Sjödin, Anders Mikael
AU - Wagner, Karl-Heinz
AU - Zeisel, Steven H
AU - Cameron-Smith, David
N1 - CURIS 2019 NEXS 329
PY - 2019
Y1 - 2019
N2 - Higher dietary protein intake is increasingly recommended for the elderly; however, high protein diets have also been linked to increased cardiovascular disease (CVD) risk. Trimethylamine-N-oxide (TMAO) is a bacterial metabolite derived from choline and carnitine abundant from animal protein-rich foods. TMAO may be a novel biomarker for heightened CVD risk. The purpose of this study was to assess the impact of a high protein diet on TMAO. Healthy men (74.2 ± 3.6 years, n = 29) were randomised to consume the recommended dietary allowance of protein (RDA: 0.8 g protein/kg bodyweight/day) or twice the RDA (2RDA) as part of a supplied diet for 10 weeks. Fasting blood samples were collected pre- and post-intervention for measurement of TMAO, blood lipids, glucose tolerance, insulin sensitivity, and inflammatory biomarkers. An oral glucose tolerance test was also performed. In comparison with RDA, the 2RDA diet increased circulatory TMAO (p = 0.002) but unexpectedly decreased renal excretion of TMAO (p = 0.003). LDL cholesterol was increased in 2RDA compared to RDA (p = 0.049), but no differences in other biomarkers of CVD risk and insulin sensitivity were evident between groups. In conclusion, circulatory TMAO is responsive to changes in dietary protein intake in older healthy males.
AB - Higher dietary protein intake is increasingly recommended for the elderly; however, high protein diets have also been linked to increased cardiovascular disease (CVD) risk. Trimethylamine-N-oxide (TMAO) is a bacterial metabolite derived from choline and carnitine abundant from animal protein-rich foods. TMAO may be a novel biomarker for heightened CVD risk. The purpose of this study was to assess the impact of a high protein diet on TMAO. Healthy men (74.2 ± 3.6 years, n = 29) were randomised to consume the recommended dietary allowance of protein (RDA: 0.8 g protein/kg bodyweight/day) or twice the RDA (2RDA) as part of a supplied diet for 10 weeks. Fasting blood samples were collected pre- and post-intervention for measurement of TMAO, blood lipids, glucose tolerance, insulin sensitivity, and inflammatory biomarkers. An oral glucose tolerance test was also performed. In comparison with RDA, the 2RDA diet increased circulatory TMAO (p = 0.002) but unexpectedly decreased renal excretion of TMAO (p = 0.003). LDL cholesterol was increased in 2RDA compared to RDA (p = 0.049), but no differences in other biomarkers of CVD risk and insulin sensitivity were evident between groups. In conclusion, circulatory TMAO is responsive to changes in dietary protein intake in older healthy males.
KW - CVD
KW - CVD biomarkers
KW - Elderly
KW - High protein diet
KW - TMAO
UR - http://www.scopus.com/inward/record.url?scp=85072567090&partnerID=8YFLogxK
U2 - 10.3390/nu11092207
DO - 10.3390/nu11092207
M3 - Journal article
C2 - 31547446
AN - SCOPUS:85072567090
VL - 11
JO - Nutrients
JF - Nutrients
SN - 2072-6643
IS - 9
M1 - 2207
ER -
ID: 228533670