TY - JOUR

T1 - Parallel determination of gut permeability in man with Mr 400, Mr 1500, Mr 4000 and Mr 10000 polyethylene glycol

AU - Parlesak, Alexandr

AU - Bode, J Christian

AU - Bode, Christiane

N1 - (Ekstern)

PY - 1994

Y1 - 1994

N2 - Polyethylene glycol has been in use for a number of years for the assessment of gut permeability. The methods so far employed are usually limited to polyethylene glycols in the low relative molecular mass range (up to Mr 1300). We developed a method for the simultaneous determination of gut permeability to Mr 400, Mr 1500, Mr 4000 and Mr 10000 polyethylene glycol, by applying a single oral dose of an appropriate mixture of these polyethylene glycols. After extraction from 24 h-urine, Mr 1500, Mr 4000 and Mr 10000 polyethylene glycol were quantified by size exclusion chromatography, while Mr 400 polyethylene glycol was determined by reversed phase chromatography. The detection limit of polyethylene glycol in the relative molecular mass range between Mr 1500 and Mr 10000 was found to be 0.2 mg/1 urine, and the detection limit of Mr 400 polyethylene glycol 5 mg/1 urine. Recovery of the polyethylene glycols (N = 6) were 86.6% (CV: 4.8%) for Mr 400, 94.1% (CV: 7.2%) for Mr 1500, 97.1% (CV: 5.5%) for Mr 4000 and 97.4% (CV: 5.6%) for Mr 10000. No significant difference was found between the excretion rates in 24 h-urine of Mr 400 and Mr 1500 polyethylene glycols in patients with Crohn's disease (Mr 400: 34.4 ± 5.5%; Mr 1500: 5.22 ± 2.27%; mean ± SEM, N = 10) and healthy controls (Mr 400: 33.6 ± 3.2%, Mr 1500: 1.09 ± 0.26%; N = 21). The excretion rate of Mr 4000 polyethylene glycol was markedly higher in patients with Crohn's disease (0.462 ± 0.177%) than in healthy controls (0.049 ± 0.012%, p < 0.05). Mr 10000 polyethylene glycol was detected in the urine of only one out of the 21 healthy controls, but in nine out of the ten patients with Crohn's disease (p < 0.001). These results suggest a change not in number but rather in selectivity in permeability of high Mr molecules.

AB - Polyethylene glycol has been in use for a number of years for the assessment of gut permeability. The methods so far employed are usually limited to polyethylene glycols in the low relative molecular mass range (up to Mr 1300). We developed a method for the simultaneous determination of gut permeability to Mr 400, Mr 1500, Mr 4000 and Mr 10000 polyethylene glycol, by applying a single oral dose of an appropriate mixture of these polyethylene glycols. After extraction from 24 h-urine, Mr 1500, Mr 4000 and Mr 10000 polyethylene glycol were quantified by size exclusion chromatography, while Mr 400 polyethylene glycol was determined by reversed phase chromatography. The detection limit of polyethylene glycol in the relative molecular mass range between Mr 1500 and Mr 10000 was found to be 0.2 mg/1 urine, and the detection limit of Mr 400 polyethylene glycol 5 mg/1 urine. Recovery of the polyethylene glycols (N = 6) were 86.6% (CV: 4.8%) for Mr 400, 94.1% (CV: 7.2%) for Mr 1500, 97.1% (CV: 5.5%) for Mr 4000 and 97.4% (CV: 5.6%) for Mr 10000. No significant difference was found between the excretion rates in 24 h-urine of Mr 400 and Mr 1500 polyethylene glycols in patients with Crohn's disease (Mr 400: 34.4 ± 5.5%; Mr 1500: 5.22 ± 2.27%; mean ± SEM, N = 10) and healthy controls (Mr 400: 33.6 ± 3.2%, Mr 1500: 1.09 ± 0.26%; N = 21). The excretion rate of Mr 4000 polyethylene glycol was markedly higher in patients with Crohn's disease (0.462 ± 0.177%) than in healthy controls (0.049 ± 0.012%, p < 0.05). Mr 10000 polyethylene glycol was detected in the urine of only one out of the 21 healthy controls, but in nine out of the ten patients with Crohn's disease (p < 0.001). These results suggest a change not in number but rather in selectivity in permeability of high Mr molecules.

U2 - 10.1515/cclm.1994.32.11.813

DO - 10.1515/cclm.1994.32.11.813

M3 - Journal article

C2 - 7888476

AN - SCOPUS:84942224097

VL - 32

SP - 813

EP - 820

JO - Clinical Chemistry and Laboratory Medicine

JF - Clinical Chemistry and Laboratory Medicine

SN - 1434-6621

IS - 11

ER -