Liquid chromatography-high resolution mass spectrometry for the analysis of phytochemicals in vegetal-derived food and beverages
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Liquid chromatography-high resolution mass spectrometry for the analysis of phytochemicals in vegetal-derived food and beverages. / La Barbera, Giorgia; Capriotti, Anna Laura; Cavaliere, Chiara; Montone, Carmela Maria; Piovesana, Susy; Samperi, Roberto; Zenezini Chiozzi, Riccardo; Laganà, Aldo.
In: Food Research International, Vol. 100, 2017, p. 28-52.Research output: Contribution to journal › Review › Research › peer-review
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TY - JOUR
T1 - Liquid chromatography-high resolution mass spectrometry for the analysis of phytochemicals in vegetal-derived food and beverages
AU - La Barbera, Giorgia
AU - Capriotti, Anna Laura
AU - Cavaliere, Chiara
AU - Montone, Carmela Maria
AU - Piovesana, Susy
AU - Samperi, Roberto
AU - Zenezini Chiozzi, Riccardo
AU - Laganà, Aldo
N1 - (Ekstern)
PY - 2017
Y1 - 2017
N2 - The recent years witnessed a change in the perception of nutrition. Diet does not only provide nutrients to meet the metabolic requirements of the body, but it also constitutes an active way for the consumption of compounds beneficial for human health. Fruit and vegetables are an excellent source of such compounds, thus the growing interest in characterizing phytochemical sources, structures and activities. Given the interest for phytochemicals in food, the development of advanced and suitable analytical techniques for their identification is fundamental for the advancement of food research. In this review, the state of the art of phytochemical research in food plants is described, starting from sample preparation, throughout extract clean-up and compound separation techniques, to the final analysis, considering both qualitative and quantitative investigations. In this regard, from an analytical point of view, fruit and vegetable extracts are complex matrices, which greatly benefit from the use of modern hyphenated techniques, in particular from the combination of high performance liquid chromatography separation and high resolution mass spectrometry, powerful tools which are being increasingly used in the recent years. Therefore, selected applications to real samples are presented and discussed, in particular for the analysis of phenols, polyphenols and phenolic acids. Finally, some hot points are discussed, such as waste characterization for high value-compounds recovery and the untargeted metabolomics approach.
AB - The recent years witnessed a change in the perception of nutrition. Diet does not only provide nutrients to meet the metabolic requirements of the body, but it also constitutes an active way for the consumption of compounds beneficial for human health. Fruit and vegetables are an excellent source of such compounds, thus the growing interest in characterizing phytochemical sources, structures and activities. Given the interest for phytochemicals in food, the development of advanced and suitable analytical techniques for their identification is fundamental for the advancement of food research. In this review, the state of the art of phytochemical research in food plants is described, starting from sample preparation, throughout extract clean-up and compound separation techniques, to the final analysis, considering both qualitative and quantitative investigations. In this regard, from an analytical point of view, fruit and vegetable extracts are complex matrices, which greatly benefit from the use of modern hyphenated techniques, in particular from the combination of high performance liquid chromatography separation and high resolution mass spectrometry, powerful tools which are being increasingly used in the recent years. Therefore, selected applications to real samples are presented and discussed, in particular for the analysis of phenols, polyphenols and phenolic acids. Finally, some hot points are discussed, such as waste characterization for high value-compounds recovery and the untargeted metabolomics approach.
KW - Food analysis
KW - High resolution liquid chromatography
KW - High resolution mass spectrometry
KW - Metabolomics
KW - Phenolics
KW - Phytochemicals
KW - Polyphenols
UR - http://www.scopus.com/inward/record.url?scp=85027100163&partnerID=8YFLogxK
U2 - 10.1016/j.foodres.2017.07.080
DO - 10.1016/j.foodres.2017.07.080
M3 - Review
C2 - 28873689
AN - SCOPUS:85027100163
VL - 100
SP - 28
EP - 52
JO - Food Research International
JF - Food Research International
SN - 0963-9969
ER -
ID: 231310494