Impaired collagen synthesis in the rectum may be a molecular target in anastomotic leakage prophylaxis
Research output: Contribution to journal › Letter › Research › peer-review
Standard
Impaired collagen synthesis in the rectum may be a molecular target in anastomotic leakage prophylaxis. / Buch, Anastasia S; Schjerling, Peter; Kjaer, Marie; Jorgensen, Lars Nannestad; Krarup, Peter-Martin; Ågren, Magnus S.
In: Wound Repair and Regeneration, Vol. 25, No. 3, 05.2017, p. 532-535.Research output: Contribution to journal › Letter › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Impaired collagen synthesis in the rectum may be a molecular target in anastomotic leakage prophylaxis
AU - Buch, Anastasia S
AU - Schjerling, Peter
AU - Kjaer, Marie
AU - Jorgensen, Lars Nannestad
AU - Krarup, Peter-Martin
AU - Ågren, Magnus S
N1 - © 2017 by the Wound Healing Society.
PY - 2017/5
Y1 - 2017/5
N2 - The underlying molecular mechanisms for anastomotic leakage (AL) after colorectal surgery are unknown and there are no therapeutics for AL prevention. Our aim was to correlate endogenous matrix metalloproteinase (MMP) activity, collagen concentration, and collagen/MMP/cytokine mRNA levels with anatomic location in human colorectal tissue. We enrolled 22 patients in this prospective study: 7 underwent elective laparoscopic sigmoid resection and 15 underwent low anterior resection for colorectal cancer. Full-thickness intestinal tissue rings from anastomoses constructed with a circular stapler were used for the determination of the MMP activity, tissue collagen concentration and mRNA levels. COL1A1 (p = 0.017) and COL3A1 (p = 0.0013) mRNA levels were lower in rectal tissue than in colonic samples. Neither MMP activities nor collagen concentrations differed significantly between the two anatomic locations. By elucidating the factors responsible for the decreased collagen production we may identify specific molecular targets in AL prophylaxis.
AB - The underlying molecular mechanisms for anastomotic leakage (AL) after colorectal surgery are unknown and there are no therapeutics for AL prevention. Our aim was to correlate endogenous matrix metalloproteinase (MMP) activity, collagen concentration, and collagen/MMP/cytokine mRNA levels with anatomic location in human colorectal tissue. We enrolled 22 patients in this prospective study: 7 underwent elective laparoscopic sigmoid resection and 15 underwent low anterior resection for colorectal cancer. Full-thickness intestinal tissue rings from anastomoses constructed with a circular stapler were used for the determination of the MMP activity, tissue collagen concentration and mRNA levels. COL1A1 (p = 0.017) and COL3A1 (p = 0.0013) mRNA levels were lower in rectal tissue than in colonic samples. Neither MMP activities nor collagen concentrations differed significantly between the two anatomic locations. By elucidating the factors responsible for the decreased collagen production we may identify specific molecular targets in AL prophylaxis.
KW - Journal Article
U2 - 10.1111/wrr.12535
DO - 10.1111/wrr.12535
M3 - Letter
C2 - 28371122
VL - 25
SP - 532
EP - 535
JO - Wound Repair and Regeneration
JF - Wound Repair and Regeneration
SN - 1067-1927
IS - 3
ER -
ID: 186908904