Cytochrome P450 levels are altered in patients with esophageal squamous-cell carcinoma
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Cytochrome P450 levels are altered in patients with esophageal squamous-cell carcinoma. / Bergheim, Ina; Wolfgarten, E; Bollschweiler, E; Hölscher, A H; Bode, Cristiana; Parlesak, Alexandr.
In: World Journal of Gastroenterology, Vol. 13, No. 7, 2007, p. 997-1002.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Cytochrome P450 levels are altered in patients with esophageal squamous-cell carcinoma
AU - Bergheim, Ina
AU - Wolfgarten, E
AU - Bollschweiler, E
AU - Hölscher, A H
AU - Bode, Cristiana
AU - Parlesak, Alexandr
N1 - (Ekstern)
PY - 2007
Y1 - 2007
N2 - Aim: To investigate the role of cytochrome P450 (CYP) in the carcinogenesis of squamous-cell carcinoma (SCC) in human esophagus by determining expression patterns and protein levels of representative CYPs in esophageal tissue of patients with SCC and controls. Methods: mRNA expression of CYP2E1, CYP2C, CYP3A4, and CYP3A5 was determined using RT-PCR in both normal and malignant esophageal tissues of patients with untreated esophageal SCC (n = 21) and in controls (n = 10). Protein levels of CYP2E1, CYP2C8, CYP3A4, and CYP3A5 were measured by Western blot. Results: Within the group of SCC patients, mRNA expression of CYP 3A4 and CYP2C was significantly lower in malignant tissue (-39% and -74%, respectively, P < 0.05) than in normal tissue. Similar results were found in CYP3A4 protein levels. Between groups, CYP3A4, CYP3A5, and CYP2C8 protein concentration was significantly higher in non-malignant tissue of SCC patients (4.8-, 2.9-, and 1.9-fold elevation, P < 0.05) than in controls. In contrast, CYP2E1 protein levels were significantly higher in controls than in SCC patients (+46%, P < 0.05). Conclusion: Significant differences exist in protein levels of certain CYPs in non-malignant esophageal tissue (e.g. CYP2C8, CYP3A4, CYP3A5, and CYP2E1) between SCC patients and healthy subjects and may contribute to the development of SCC in the esophagus.
AB - Aim: To investigate the role of cytochrome P450 (CYP) in the carcinogenesis of squamous-cell carcinoma (SCC) in human esophagus by determining expression patterns and protein levels of representative CYPs in esophageal tissue of patients with SCC and controls. Methods: mRNA expression of CYP2E1, CYP2C, CYP3A4, and CYP3A5 was determined using RT-PCR in both normal and malignant esophageal tissues of patients with untreated esophageal SCC (n = 21) and in controls (n = 10). Protein levels of CYP2E1, CYP2C8, CYP3A4, and CYP3A5 were measured by Western blot. Results: Within the group of SCC patients, mRNA expression of CYP 3A4 and CYP2C was significantly lower in malignant tissue (-39% and -74%, respectively, P < 0.05) than in normal tissue. Similar results were found in CYP3A4 protein levels. Between groups, CYP3A4, CYP3A5, and CYP2C8 protein concentration was significantly higher in non-malignant tissue of SCC patients (4.8-, 2.9-, and 1.9-fold elevation, P < 0.05) than in controls. In contrast, CYP2E1 protein levels were significantly higher in controls than in SCC patients (+46%, P < 0.05). Conclusion: Significant differences exist in protein levels of certain CYPs in non-malignant esophageal tissue (e.g. CYP2C8, CYP3A4, CYP3A5, and CYP2E1) between SCC patients and healthy subjects and may contribute to the development of SCC in the esophagus.
KW - Cytochrome P450
KW - Human
KW - RT-PCR
KW - Squamos-cell carcinoma
KW - Western blot
U2 - 10.3748/wjg.v13.i7.997
DO - 10.3748/wjg.v13.i7.997
M3 - Journal article
C2 - 17373732
AN - SCOPUS:34047176473
VL - 13
SP - 997
EP - 1002
JO - World Chinese Journal of Digestology
JF - World Chinese Journal of Digestology
SN - 1009-3079
IS - 7
ER -
ID: 322184967