Antibiotic treatment preventing necrotising enterocolitis alters urinary and plasma metabolomes in preterm pigs
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Antibiotic treatment preventing necrotising enterocolitis alters urinary and plasma metabolomes in preterm pigs. / Jiang, Pingping; Trimigno, Alessia; Stanstrup, Jan; Khakimov, Bekzod; Viereck, Nanna; Engelsen, Søren Balling; Sangild, Per Torp; Dragsted, Lars Ove.
In: Journal of Proteome Research, Vol. 16, 2017, p. 3547-3557.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Antibiotic treatment preventing necrotising enterocolitis alters urinary and plasma metabolomes in preterm pigs
AU - Jiang, Pingping
AU - Trimigno, Alessia
AU - Stanstrup, Jan
AU - Khakimov, Bekzod
AU - Viereck, Nanna
AU - Engelsen, Søren Balling
AU - Sangild, Per Torp
AU - Dragsted, Lars Ove
N1 - CURIS 2017 NEXS 245
PY - 2017
Y1 - 2017
N2 - Necrotising enterocolitis (NEC) is a serious gut inflammatory condition in premature neonates, onset and development of which depends on the gut microbiome. Attenuation of the gut microbiome by antibiotics can reduce NEC incidence and severity. However, how the antibiotics-suppressed gut microbiome affects the whole-body metabolism in NEC-sensitive premature neonates is unknown. In formula-fed preterm pigs, used as a model for preterm infants, plasma and urinary metabolomes were investigated by LC-MS and (1)H-NMR, with and without antibiotic treatment immediately after birth. While reducing the gut microbiome density and NEC lesions as previously reported, the antibiotic treatment employed in the current study affected the abundance of 44 metabolites in different metabolic pathways. In antibiotics-treated pigs, tryptophan metabolism favoured the kynurenine pathway, relative to the serotonin pathway, as shown by specific metabolites. Metabolites associated with the gut microbiome, including 3-phenyllactic acid, 4-hydroxyphenylacetic acid and phenylacetylglycine, all from phenylalanine, and three bile acids showed lower levels in the antibiotics-treated pigs where the gut microbiome was extensively attenuated. Findings in the current study warrant further investigation of metabolic and developmental consequences of antibiotic treatment in preterm neonates.
AB - Necrotising enterocolitis (NEC) is a serious gut inflammatory condition in premature neonates, onset and development of which depends on the gut microbiome. Attenuation of the gut microbiome by antibiotics can reduce NEC incidence and severity. However, how the antibiotics-suppressed gut microbiome affects the whole-body metabolism in NEC-sensitive premature neonates is unknown. In formula-fed preterm pigs, used as a model for preterm infants, plasma and urinary metabolomes were investigated by LC-MS and (1)H-NMR, with and without antibiotic treatment immediately after birth. While reducing the gut microbiome density and NEC lesions as previously reported, the antibiotic treatment employed in the current study affected the abundance of 44 metabolites in different metabolic pathways. In antibiotics-treated pigs, tryptophan metabolism favoured the kynurenine pathway, relative to the serotonin pathway, as shown by specific metabolites. Metabolites associated with the gut microbiome, including 3-phenyllactic acid, 4-hydroxyphenylacetic acid and phenylacetylglycine, all from phenylalanine, and three bile acids showed lower levels in the antibiotics-treated pigs where the gut microbiome was extensively attenuated. Findings in the current study warrant further investigation of metabolic and developmental consequences of antibiotic treatment in preterm neonates.
KW - Antibiotics
KW - Metabolomics
KW - Necrotising enterocolitis
KW - UPLC-MS
KW - NMR
KW - Preterm pigs
U2 - 10.1021/acs.jproteome.7b00263
DO - 10.1021/acs.jproteome.7b00263
M3 - Journal article
C2 - 28871782
VL - 16
SP - 3547
EP - 3557
JO - Journal of Proteome Research
JF - Journal of Proteome Research
SN - 1535-3893
ER -
ID: 183010145